(Circular) (Six-base) MAPSORT of: pFN21AE3112.seq Check: 7576 from: 1 to: 5557 pFN21AE3112 5557 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3367 278 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2900 1937 Size: 963 4594 Acc65I G'GTAC_C Cuts at: 2878 2878 Size: 5557 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3620 5004 5272 1632 Size: 1988 1384 268 1917 Fragments arranged by size: 1988 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5360 AflIII A'CryG_T Cuts at: 1203 4456 5433 1203 Size: 3253 977 1327 Fragments arranged by size: 3253 1327 977 AgeI A'CCGG_T Cuts at: 4388 4388 Size: 5557 AhdI GACnn_n'nnGTC Cuts at: 4286 4286 Size: 5557 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5557 AlwNI CAG_nnn'CTG Cuts at: 2347 3300 4872 2347 Size: 953 1572 3032 Fragments arranged by size: 3032 1572 953 ApaLI G'TGCA_C Cuts at: 3614 4770 3614 Size: 1156 4401 ApoI r'AATT_y Cuts at: 1493 2263 2866 3092 3244 5353 1493 Size: 770 603 226 152 2109 1697 Fragments arranged by size: 2109 1697 770 603 226 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2652 2882 1870 Size: 73 709 230 4545 Fragments arranged by size: 4545 709 230 73 BamHI G'GATC_C Cuts at: 2887 3254 2887 Size: 367 5190 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2878 4333 618 Size: 325 217 627 1091 1455 1842 Fragments arranged by size: 1842 1455 1091 627 325 217 BanII G_rGCy'C Cuts at: 1391 2713 2876 1391 Size: 1322 163 4072 Fragments arranged by size: 4072 1322 163 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2163 928 Size: 971 264 4322 Fragments arranged by size: 4322 971 264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3748 3782 1903 Size: 34 1811 34 3678 Fragments arranged by size: 3678 1811 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2641 3449 4659 2641 Size: 808 1210 3539 Fragments arranged by size: 3539 1210 808 BclI T'GATC_A Cuts at: 1548 1548 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4168 136 Size: 107 122 71 631 605 2496 1525 Fragments arranged by size: 2496 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5552 5552 Size: 5557 BlpI GC'TnA_GC Cuts at: 2974 2974 Size: 5557 Bme1580I G_kGCm'C Cuts at: 1790 3618 4774 1790 Size: 1828 1156 2573 Fragments arranged by size: 2573 1828 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5557 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2593 2835 3288 Size: 416 53 83 399 24 1176 242 453 Cuts at: 3288 4246 442 Size: 958 1753 Fragments arranged by size: 1753 1176 958 453 416 399 242 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5557 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2809 2809 Size: 5557 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2698 4217 1239 Size: 1459 1519 2579 Fragments arranged by size: 2579 1519 1459 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3682 4547 4845 3682 Size: 865 298 4394 Fragments arranged by size: 4394 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4220 882 Size: 617 2721 2219 Fragments arranged by size: 2721 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2032 3348 493 Size: 711 828 1316 2702 Fragments arranged by size: 2702 1316 828 711 BsaBI GATnn'nnATC Cuts at: 5551 5551 Size: 5557 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3364 3746 4381 275 Size: 53 83 186 936 1831 382 635 1451 Fragments arranged by size: 1831 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3990 4388 4662 4809 5220 3990 Size: 398 274 147 411 4327 Fragments arranged by size: 4327 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2700 2730 1178 Size: 30 165 30 1297 30 4005 Fragments arranged by size: 4005 1297 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2127 2801 1186 Size: 941 674 3942 Fragments arranged by size: 3942 941 674 BseYI C'CCAG_C Cuts at: 4760 5256 4760 Size: 496 5061 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2349 1163 Size: 573 613 4371 Fragments arranged by size: 4371 613 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3007 3768 4796 1540 Size: 275 186 1006 761 1028 2301 Fragments arranged by size: 2301 1028 1006 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2049 2713 3618 3703 4774 2049 Size: 664 905 85 1071 2832 Fragments arranged by size: 2832 1071 905 664 85 BsiWI C'GTAC_G Cuts at: 2028 2028 Size: 5557 BsmI GAATG_Cn' Cuts at: 2476 3068 3161 2476 Size: 592 93 4872 Fragments arranged by size: 4872 592 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2713 2876 3618 3703 4774 1391 Size: 399 259 664 163 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 664 399 259 163 85 BspEI T'CCGG_A Cuts at: 5220 5220 Size: 5557 BspHI T'CATG_A Cuts at: 3446 5176 3446 Size: 1730 3827 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2484 2912 844 Size: 1640 428 3489 Fragments arranged by size: 3489 1640 428 BsrBI CCG'CTC Cuts at: 1394 1823 3444 1394 Size: 429 1621 3507 Fragments arranged by size: 3507 1621 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2355 4052 4226 65 Size: 1183 1107 1697 174 1396 Fragments arranged by size: 1697 1396 1183 1107 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4201 4388 1700 Size: 110 2391 187 2869 Fragments arranged by size: 2869 2391 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5557 BssSI C'ACGA_G Cuts at: 3617 4629 3617 Size: 1012 4545 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5557 BstBI TT'CG_AA Cuts at: 4361 4361 Size: 5557 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5557 BstXI CCAn_nnnn'nTGG Cuts at: 2641 2641 Size: 5557 BstYI r'GATC_y Cuts at: 1972 2887 3254 3639 3656 5108 5194 5206 5552 Size: 915 367 385 17 1452 86 12 346 Cuts at: 5552 1972 Size: 1977 Fragments arranged by size: 1977 1452 915 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5005 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2451 2697 487 Size: 683 56 1225 246 3347 Fragments arranged by size: 3347 1225 683 246 56 BtsI GCAGTG_nn' Cuts at: 2606 3073 3867 3887 2606 Size: 467 794 20 4276 Fragments arranged by size: 4276 794 467 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5557 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5522 DraI TTT'AAA Cuts at: 2773 2861 2773 Size: 88 5469 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4564 809 Size: 507 3248 1802 Fragments arranged by size: 3248 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3004 3893 5522 Size: 54 1425 50 275 18 1174 889 1629 Cuts at: 5522 8 Size: 43 Fragments arranged by size: 1629 1425 1174 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3004 1537 Size: 275 1192 4090 Fragments arranged by size: 4090 1192 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2524 3487 1353 Size: 1171 963 3423 Fragments arranged by size: 3423 1171 963 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4144 4518 4664 387 Size: 1064 138 2555 374 146 1280 Fragments arranged by size: 2555 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2711 2711 Size: 5557 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2698 3620 4217 5004 5272 1239 Size: 393 1066 922 597 787 268 1524 Fragments arranged by size: 1524 1066 922 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2272 2272 Size: 5557 EcoRI G'AATT_C Cuts at: 2866 3244 2866 Size: 378 5179 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2784 2784 Size: 5557 FspI TGC'GCA Cuts at: 4063 4063 Size: 5557 HaeII r_GCGC'y Cuts at: 1678 4704 1678 Size: 3026 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373 Size: 32 135 32 2626 32 42 32 2626 Fragments arranged by size: 2626 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2691 2901 3147 669 Size: 988 281 753 210 246 3079 Fragments arranged by size: 3079 988 753 281 246 210 HindIII A'AGCT_T Cuts at: 748 748 Size: 5557 HpaI GTT'AAC Cuts at: 3147 3147 Size: 5557 KpnI G_GTAC'C Cuts at: 2882 2882 Size: 5557 MfeI C'AATT_G Cuts at: 3156 3156 Size: 5557 MluI A'CGCG_T Cuts at: 5433 5433 Size: 5557 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2131 4671 4855 1305 Size: 826 2540 184 2007 Fragments arranged by size: 2540 2007 826 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3735 Fragments arranged by size: 3735 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2639 3515 3874 4033 518 Size: 648 1473 876 359 159 2042 Fragments arranged by size: 2042 1473 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2333 2973 3650 4798 5043 1606 Size: 727 640 677 1148 245 2120 Fragments arranged by size: 2120 1148 727 677 640 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5005 NdeI CA'TA_TG Cuts at: 387 2459 387 Size: 2072 3485 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5557 NotI GC'GGCC_GC Cuts at: 3004 3004 Size: 5557 NspI r_CATG'y Cuts at: 1132 1229 2542 2915 4460 1132 Size: 97 1313 373 1545 2229 Fragments arranged by size: 2229 1545 1313 373 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5557 PciI A'CATG_T Cuts at: 4456 4456 Size: 5557 PflMI CCAn_nnn'nTGG Cuts at: 1108 2825 1108 Size: 1717 3840 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3626 3658 5139 5171 3626 Size: 32 1481 32 4012 Fragments arranged by size: 4012 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5557 PsiI TTA'TAA Cuts at: 2667 3127 2667 Size: 460 5097 PstI C_TGCA'G Cuts at: 830 2435 2909 830 Size: 1605 474 3478 Fragments arranged by size: 3478 1605 474 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5557 PvuII CAG'CTG Cuts at: 2333 2333 Size: 5557 SacI G_AGCT'C Cuts at: 2713 2713 Size: 5557 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SbfI CC_TGCA'GG Cuts at: 2909 2909 Size: 5557 ScaI AGT'ACT Cuts at: 3805 5517 3805 Size: 1712 3845 SfcI C'TryA_G Cuts at: 826 1045 2431 2905 4040 4721 4912 826 Size: 219 1386 474 1135 681 191 1471 Fragments arranged by size: 1471 1386 1135 681 474 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2884 1872 Size: 1012 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2652 3661 4562 4824 820 Size: 197 926 709 1009 901 262 1553 Fragments arranged by size: 1553 1009 926 901 709 262 197 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2542 2915 1132 Size: 1410 373 3774 Fragments arranged by size: 3774 1410 373 SspI AAT'ATT Cuts at: 5 52 2724 3481 5 Size: 47 2672 757 2081 Fragments arranged by size: 2672 2081 757 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5557 StyI C'CwwG_G Cuts at: 513 1065 2996 513 Size: 552 1931 3074 Fragments arranged by size: 3074 1931 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3584 3601 3754 3939 4384 325 Size: 1255 2004 17 153 185 445 1498 Fragments arranged by size: 2004 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 3803 5515 Size: 274 80 33 51 1443 555 1271 1712 Cuts at: 5515 96 Size: 138 Fragments arranged by size: 1712 1443 1271 555 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2249 2281 5517 5549 2249 Size: 32 3236 32 2257 Fragments arranged by size: 3236 2257 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2893 2893 Size: 5557 XhoI C'TCGA_G Cuts at: 1943 2652 1943 Size: 709 4848 XmaI C'CCGG_G Cuts at: 1870 2882 1870 Size: 1012 4545 XmnI GAAnn'nnTTC Cuts at: 3686 3686 Size: 5557 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3365 276 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI