################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AspEI AspI AviII AvrII BamHI BbeI BcgI BciVI BfrI BlnI BsaHI BsaI BsiWI BspDI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I ClaI CpoI DraIII Eam1105I Eco47III Eco81I EcoNI FspI HgaI Hin1I KasI MluI MslI NarI NheI NruI NspV PacI PflFI PflMI PinAI PmeI PvuI RsrII SacI SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 70 enzymes) # ################################### Acc65I 1812 GGTACC G^GTACC AccII 39 CGCG CG^CG AccIII 1988 TCCGGA T^CCGGA AclI 2171 AACGTT AA^CGTT AflIII 3033 ACGTGT A^C[GA][CT]GT AlwNI 1309 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 1812 GGTACC G^GTACC AspHI 2450 GAGCAC G[AT]GC[AT]^C BalI 3383 TGGCCA TGG^CCA BanII 169 GAGCCC G[GA]GC[CT]^C BbrPI 1460 CACGTG CAC^GTG BbvCI 3121 CCTCAGC CCTCAGC(-5/-2) BglI 97 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3363 AGATCT A^GATCT BlpI 1306 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1306 GCTCAGC GC^T[ACGT]AGC BsaWI 1988 TCCGGA [AT]^CCGG[AT] BseAI 1988 TCCGGA T^CCGGA BsgI 1601 CTGCAC GTGCAG(16/14) BsiEI 35 CGGCCG CG[GA][CT]^CG BsiHKAI 2450 GAGCAC G[AT]GC[AT]^C BspEI 1988 TCCGGA T^CCGGA BspMI 1780 GCAGGT ACCTGC(4/8) BstAPI 3361 GCAGATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 457 GGTGACC G^GT[ACGT]ACC BstPI 457 GGTGACC G^GT[ACGT]ACC BstUI 39 CGCG CG^CG CelII 1306 GCTCAGC GC^T[ACGT]AGC CfoI 2931 GCGC GCG^C DrdI 3315 GACAGAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 35 CGGCCG C^GGCCG EclXI 35 CGGCCG C^GGCCG Eco52I 35 CGGCCG C^GGCCG EcoO65I 457 GGTGACC G^GT[ACGT]ACC EcoRV 1979 GATATC GAT^ATC EcoT22I 3419 ATGCAT ATGCA^T FauI 2949 GCGGG CCCGC(4/6) FseI 131 GGCCGGCC GGCCGG^CC HaeII 2930 AGCGCC [GA]GCGC^[CT] HhaI 2931 GCGC GCG^C HinP1I 2931 GCGC G^CGC HincII 3513 GTTAAC GT[CT]^[GA]AC HindII 3513 GTTAAC GT[CT]^[GA]AC HindIII 4027 AAGCTT A^AGCTT HpaI 3513 GTTAAC GTT^AAC KpnI 1812 GGTACC GGTAC^C KspI 38 CCGCGG CCGC^GG MluNI 3383 TGGCCA TGG^CCA MroI 1988 TCCGGA T^CCGGA MscI 3383 TGGCCA TGG^CCA MvnI 39 CGCG CG^CG NcoI 3386 CCATGG C^CATGG NdeI 626 CATATG CA^TATG NotI 34 GCGGCCGC GC^GGCCGC NsiI 3419 ATGCAT ATGCA^T PaeR7I 2000 CTCGAG C^TCGAG PmaCI 1460 CACGTG CAC^GTG PmlI 1460 CACGTG CAC^GTG PpuMI 635 AGGACCC [GA]G^G[AT]CC[CT] PshAI 2901 GACGTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2171 AACGTT AA^CGTT SacII 38 CCGCGG CCGC^GG ScaI 1804 AGTACT AGT^ACT SexAI 2017 ACCAGGT A^CC[AT]GGT SmlI 2000 CTCGAG C^T[CT][GA]AG StuI 559 AGGCCT AGG^CCT SwaI 2462 ATTTAAAT ATTT^AAAT Tsp45I 458 GTGAC ^GT[GC]AC XcmI 2934 CCAGGGAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2000 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AseI 268 ATTAAT AT^TAAT 2386 ATTAAT AsnI 268 ATTAAT AT^TAAT 2386 ATTAAT AvaI 1558 CCCGAG C^[CT]CG[GA]G 2000 CTCGAG BanI 1812 GGTACC G^G[CT][GA]CC 3265 GGTGCC BclI 302 TGATCA T^GATCA 3134 TGATCA BcnI 48 CCGGG CC^[GC]GG 98 CCGGG BmyI 169 GAGCCC G[AGT]GC[ACT]^C 2450 GAGCAC BsaAI 1460 CACGTG [CT]AC^GT[GA] 3032 TACGTG BsaBI 2461 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3566 GATTTTCATC BsoBI 1558 CCCGAG C^[CT]CG[GA]G 2000 CTCGAG Bsp1286I 169 GAGCCC G[AGT]GC[ACT]^C 2450 GAGCAC Bsp1407I 2032 TGTACA T^GTACA 2978 TGTACA BspHI 1083 TCATGA T^CATGA 2112 TCATGA BsrFI 132 GCCGGC [GA]^CCGG[CT] 157 GCCGGC BsrGI 2032 TGTACA T^GTACA 2978 TGTACA Cfr10I 132 GCCGGC [GA]^CCGG[CT] 157 GCCGGC DraII 635 AGGACCC [GA]G^G[ACGT]CC[CT] 3959 AGGCCCC DsaI 38 CCGCGG C^C[GA][CT]GG 3386 CCATGG EcoO109I 635 AGGACCC [GA]G^G[ACGT]CC[CT] 3959 AGGCCCC EcoRI 741 GAATTC G^AATTC 1965 GAATTC FbaI 302 TGATCA T^GATCA 3134 TGATCA MamI 2461 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3566 GATTTTCATC MfeI 1739 CAATTG C^AATTG 3341 CAATTG MunI 1739 CAATTG C^AATTG 3341 CAATTG NaeI 132 GCCGGC GCC^GGC 157 GCCGGC NciI 48 CCGGG CC^[GC]GG 98 CCGGG NgoMIV 132 GCCGGC G^CCGGC 157 GCCGGC PshBI 268 ATTAAT AT^TAAT 2386 ATTAAT RcaI 1083 TCATGA T^CATGA 2112 TCATGA SapI 538 GAAGAGC GCTCTTC(1/4) 2939 GAAGAGC SphI 1298 GCATGC GCATG^C 2502 GCATGC SspBI 2032 TGTACA T^GTACA 2978 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BbsI BbvI BfaI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI