(Circular) (Six-base) MAPSORT of: pFN21AE0898.seq Check: 3670 from: 1 to: 8812 pFN21AE0898 8812 bp With 182 enzymes: SgfI * September 16, 2010 15:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3776 3776 Size: 8812 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6622 278 Size: 53 83 186 936 5086 2468 Fragments arranged by size: 5086 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6155 1937 Size: 4218 4594 Acc65I G'GTAC_C Cuts at: 3817 6133 3817 Size: 2316 6496 AclI AA'CG_TT Cuts at: 1563 4177 6111 6939 7312 1563 Size: 2614 1934 828 373 3063 Fragments arranged by size: 3063 2614 1934 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2541 3078 3276 3576 5115 6875 8259 8527 Size: 909 537 198 300 1539 1760 1384 268 Cuts at: 8527 1632 Size: 1917 Fragments arranged by size: 1917 1760 1539 1384 909 537 300 268 198 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8615 AflIII A'CryG_T Cuts at: 1203 5038 7711 8688 1203 Size: 3835 2673 977 1327 Fragments arranged by size: 3835 2673 1327 977 AgeI A'CCGG_T Cuts at: 7643 7643 Size: 8812 AhdI GACnn_n'nnGTC Cuts at: 7541 7541 Size: 8812 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8812 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8812 AlwNI CAG_nnn'CTG Cuts at: 3319 6555 8127 3319 Size: 3236 1572 4004 Fragments arranged by size: 4004 3236 1572 ApaLI G'TGCA_C Cuts at: 6869 8025 6869 Size: 1156 7656 ApoI r'AATT_y Cuts at: 1493 2199 2537 2570 2746 2774 2900 3022 3100 Size: 706 338 33 176 28 126 122 78 Cuts at: 3100 3580 3970 4164 4226 4263 4361 5155 6121 Size: 480 390 194 62 37 98 794 966 Cuts at: 6121 6347 6499 8608 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 966 794 706 480 390 338 226 194 176 152 126 122 98 78 62 37 33 28 AseI AT'TA_AT Cuts at: 160 1032 2274 4392 7366 160 Size: 872 1242 2118 2974 1606 Fragments arranged by size: 2974 2118 1606 1242 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8812 AvaI C'yCGr_G Cuts at: 1870 1943 3563 4005 6137 1870 Size: 73 1620 442 2132 4545 Fragments arranged by size: 4545 2132 1620 442 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3671 3704 3801 3834 3671 Size: 33 97 33 8649 Fragments arranged by size: 8649 97 33 33 BamHI G'GATC_C Cuts at: 6142 6509 6142 Size: 367 8445 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3817 5270 6133 7588 618 Size: 325 217 627 2030 1453 863 1455 1842 Fragments arranged by size: 2030 1842 1455 1453 863 627 325 217 BanII G_rGCy'C Cuts at: 1391 2178 6131 1391 Size: 787 3953 4072 Fragments arranged by size: 4072 3953 787 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2362 2934 4070 5924 928 Size: 971 463 572 1136 1854 3816 Fragments arranged by size: 3816 1854 1136 971 572 463 BbvCI CC'TCA_GC Cuts at: 5127 5127 Size: 8812 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7003 7037 1903 Size: 34 5066 34 3678 Fragments arranged by size: 5066 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6704 7914 6704 Size: 1210 7602 BclI T'GATC_A Cuts at: 1548 2307 5139 1548 Size: 759 2832 5221 Fragments arranged by size: 5221 2832 759 BfrBI ATG'CAT Cuts at: 5426 5426 Size: 8812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2108 7423 136 Size: 107 122 71 631 605 436 5315 1525 Fragments arranged by size: 5315 1525 631 605 436 122 107 71 BglII A'GATC_T Cuts at: 5368 8807 5368 Size: 3439 5373 BlpI GC'TnA_GC Cuts at: 3312 6229 3312 Size: 2917 5895 Bme1580I G_kGCm'C Cuts at: 1790 6873 8029 1790 Size: 5083 1156 2573 Fragments arranged by size: 5083 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4436 5288 6543 Size: 416 53 83 399 24 3019 852 1255 Cuts at: 6543 7501 442 Size: 958 1753 Fragments arranged by size: 3019 1753 1255 958 852 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2019 2152 3425 3981 4473 4749 7472 1239 Size: 780 133 1273 556 492 276 2723 2579 Fragments arranged by size: 2723 2579 1273 780 556 492 276 133 Bpu10I CC'TnA_GC Cuts at: 2883 4055 5127 2883 Size: 1172 1072 6568 Fragments arranged by size: 6568 1172 1072 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6937 7802 8100 6937 Size: 865 298 7649 Fragments arranged by size: 7649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7475 882 Size: 617 5976 2219 Fragments arranged by size: 5976 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3467 5039 6603 493 Size: 711 2263 1572 1564 2702 Fragments arranged by size: 2702 2263 1572 1564 711 BsaBI GATnn'nnATC Cuts at: 4470 5575 8806 4470 Size: 1105 3231 4476 Fragments arranged by size: 4476 3231 1105 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6619 7001 7636 275 Size: 53 83 186 936 5086 382 635 1451 Fragments arranged by size: 5086 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3993 7245 7643 7917 8064 8475 3993 Size: 3252 398 274 147 411 4330 Fragments arranged by size: 4330 3252 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8587 Fragments arranged by size: 8587 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2571 2780 5115 1186 Size: 1385 209 2335 4883 Fragments arranged by size: 4883 2335 1385 209 BseYI C'CCAG_C Cuts at: 2947 4949 5598 8015 8511 2947 Size: 2002 649 2417 496 3248 Fragments arranged by size: 3248 2417 2002 649 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3591 1163 Size: 573 1855 6384 Fragments arranged by size: 6384 1855 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2043 6262 7023 8051 1540 Size: 275 186 42 4219 761 1028 2301 Fragments arranged by size: 4219 2301 1028 761 275 186 42 BsiHKAI G_wGCw'C Cuts at: 4459 6873 6958 8029 4459 Size: 2414 85 1071 5242 Fragments arranged by size: 5242 2414 1071 85 BsmI GAATG_Cn' Cuts at: 3529 4714 4748 6323 6416 3529 Size: 1185 34 1575 93 5925 Fragments arranged by size: 5925 1575 1185 93 34 BsmBI CGTCTCn'nnnn_ Cuts at: 4799 4898 5741 4799 Size: 99 843 7870 Fragments arranged by size: 7870 843 99 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2178 4459 6131 6873 6958 8029 1391 Size: 399 388 2281 1672 742 85 1071 2174 Fragments arranged by size: 2281 2174 1672 1071 742 399 388 85 BspEI T'CCGG_A Cuts at: 3993 8475 3993 Size: 4482 4330 BspHI T'CATG_A Cuts at: 3088 4117 6701 8431 3088 Size: 1029 2584 1730 3469 Fragments arranged by size: 3469 2584 1730 1029 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3776 6167 844 Size: 2932 2391 3489 Fragments arranged by size: 3489 2932 2391 BsrBI CCG'CTC Cuts at: 1394 1823 6699 1394 Size: 429 4876 3507 Fragments arranged by size: 4876 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2609 5152 5157 7307 7481 65 Size: 1183 1361 2543 5 2150 174 1396 Fragments arranged by size: 2543 2150 1396 1361 1183 174 5 BsrFI r'CCGG_y Cuts at: 1700 1810 2137 2162 7456 7643 1700 Size: 110 327 25 5294 187 2869 Fragments arranged by size: 5294 2869 327 187 110 25 BsrGI T'GTAC_A Cuts at: 96 4037 4983 96 Size: 3941 946 3925 Fragments arranged by size: 3941 3925 946 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8812 BssSI C'ACGA_G Cuts at: 6872 7884 6872 Size: 1012 7800 BstAPI GCAn_nnn'nTGC Cuts at: 794 5372 794 Size: 4578 4234 BstBI TT'CG_AA Cuts at: 7616 7616 Size: 8812 BstEII G'GTnAC_C Cuts at: 1184 2462 1184 Size: 1278 7534 BstYI r'GATC_y Cuts at: 1972 3559 3990 4546 5119 5368 5952 6142 6509 Size: 1587 431 556 573 249 584 190 367 Cuts at: 6509 6894 6911 8363 8449 8461 8807 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1587 1452 584 573 556 431 385 367 346 249 190 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 5391 513 Size: 552 939 39 3348 3934 Fragments arranged by size: 3934 3348 939 552 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8073 Fragments arranged by size: 8073 683 56 BtsI GCAGTG_nn' Cuts at: 4246 6328 7122 7142 4246 Size: 2082 794 20 5916 Fragments arranged by size: 5916 2082 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5329 5364 5694 5729 529 Size: 35 4765 35 330 35 3612 Fragments arranged by size: 4765 3612 330 35 35 35 DraI TTT'AAA Cuts at: 2848 3730 4470 6116 2848 Size: 882 740 1646 5544 Fragments arranged by size: 5544 1646 882 740 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 7819 809 Size: 507 4010 2493 1802 Fragments arranged by size: 4010 2493 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2139 5388 Size: 54 1425 50 275 18 210 99 3249 Cuts at: 5388 6259 7148 8777 8 Size: 871 889 1629 43 Fragments arranged by size: 3249 1629 1425 889 871 275 210 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2040 6259 1537 Size: 275 228 4219 4090 Fragments arranged by size: 4219 4090 275 228 EarI CTCTTCn'nnn_ Cuts at: 1353 2538 2631 2757 3057 4365 4939 6742 1353 Size: 1185 93 126 300 1308 574 1803 3423 Fragments arranged by size: 3423 1803 1308 1185 574 300 126 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5411 7399 7773 7919 387 Size: 1064 138 3822 1988 374 146 1280 Fragments arranged by size: 3822 1988 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2019 2152 2541 3078 3276 3425 3576 Size: 393 387 133 389 537 198 149 151 Cuts at: 3576 3981 4473 4749 5115 6875 7472 8259 8527 Size: 405 492 276 366 1760 597 787 268 Cuts at: 8527 1239 Size: 1524 Fragments arranged by size: 1760 1524 787 597 537 492 405 393 389 387 366 276 268 198 151 149 133 EcoO109I rG'GnC_Cy Cuts at: 2641 5965 2641 Size: 3324 5488 EcoRI G'AATT_C Cuts at: 2746 3970 6121 6499 2746 Size: 1224 2151 378 5059 Fragments arranged by size: 5059 2151 1224 378 EcoRV GAT'ATC Cuts at: 3986 3986 Size: 8812 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3860 4094 5387 3860 Size: 234 1293 7285 Fragments arranged by size: 7285 1293 234 FseI GG_CCGG'CC Cuts at: 2141 2141 Size: 8812 FspI TGC'GCA Cuts at: 7318 7318 Size: 8812 HaeII r_GCGC'y Cuts at: 1678 4939 7959 1678 Size: 3261 3020 2531 Fragments arranged by size: 3261 3020 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2950 2982 3175 3207 3975 Size: 32 135 32 1378 32 193 32 768 Cuts at: 3975 3988 4007 4020 4649 4681 5090 5122 7453 Size: 13 19 13 629 32 409 32 2331 Cuts at: 7453 7485 7527 7559 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2331 1378 768 629 409 193 135 42 32 32 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 5520 6156 6402 669 Size: 988 281 3582 636 246 3079 Fragments arranged by size: 3582 3079 988 636 281 246 HindIII A'AGCT_T Cuts at: 748 6032 748 Size: 5284 3528 HpaI GTT'AAC Cuts at: 5520 6402 5520 Size: 882 7930 KpnI G_GTAC'C Cuts at: 3821 6137 3821 Size: 2316 6496 MfeI C'AATT_G Cuts at: 3744 5346 6411 3744 Size: 1602 1065 6145 Fragments arranged by size: 6145 1602 1065 MluI A'CGCG_T Cuts at: 8688 8688 Size: 8812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3104 7926 8110 1305 Size: 1799 4822 184 2007 Fragments arranged by size: 4822 2007 1799 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5390 10 Size: 54 1425 343 3558 3432 Fragments arranged by size: 3558 3432 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 6770 7129 7288 518 Size: 648 5604 359 159 2042 Fragments arranged by size: 5604 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2045 2147 2153 2159 3050 5598 5998 Size: 433 6 102 6 6 891 2548 400 Cuts at: 5998 6228 6905 8053 8298 1606 Size: 230 677 1148 245 2120 Fragments arranged by size: 2548 2120 1148 891 677 433 400 245 230 102 6 6 6 NaeI GCC'GGC Cuts at: 2139 2164 2139 Size: 25 8787 NcoI C'CATG_G Cuts at: 513 1065 2004 5391 513 Size: 552 939 3387 3934 Fragments arranged by size: 3934 3387 939 552 NdeI CA'TA_TG Cuts at: 387 2632 387 Size: 2245 6567 NgoMIV G'CCGG_C Cuts at: 2137 2162 2137 Size: 25 8787 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8812 NotI GC'GGCC_GC Cuts at: 2040 6259 2040 Size: 4219 4593 NsiI A_TGCA'T Cuts at: 5428 5428 Size: 8812 NspI r_CATG'y Cuts at: 1132 1229 3307 4463 4511 6170 7715 1132 Size: 97 2078 1156 48 1659 1545 2229 Fragments arranged by size: 2229 2078 1659 1545 1156 97 48 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8812 PciI A'CATG_T Cuts at: 7711 7711 Size: 8812 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8812 PfoI T'CCnGG_A Cuts at: 4765 4765 Size: 8812 PmeI GTTT'AAAC Cuts at: 6116 6116 Size: 8812 PmlI CAC'GTG Cuts at: 3467 3467 Size: 8812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6881 6913 8394 8426 6881 Size: 32 1481 32 7267 Fragments arranged by size: 7267 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2641 2641 Size: 8812 PshAI GACnn'nnGTC Cuts at: 1613 4910 1613 Size: 3297 5515 PsiI TTA'TAA Cuts at: 6382 6382 Size: 8812 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4098 4130 5071 5103 4098 Size: 32 941 32 7807 Fragments arranged by size: 7807 941 32 32 PstI C_TGCA'G Cuts at: 830 3049 4747 5314 6078 6164 830 Size: 2219 1698 567 764 86 3478 Fragments arranged by size: 3478 2219 1698 764 567 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8812 PvuII CAG'CTG Cuts at: 3050 5598 5998 3050 Size: 2548 400 5864 Fragments arranged by size: 5864 2548 400 SacII CC_GC'GG Cuts at: 2046 2046 Size: 8812 SalI G'TCGA_C Cuts at: 1936 6154 1936 Size: 4218 4594 SapI GCTCTTCn'nnn_ Cuts at: 2538 4939 2538 Size: 2401 6411 SbfI CC_TGCA'GG Cuts at: 6164 6164 Size: 8812 ScaI AGT'ACT Cuts at: 3811 7060 8772 3811 Size: 3249 1712 3851 Fragments arranged by size: 3851 3249 1712 SexAI A'CCwGG_T Cuts at: 4022 4022 Size: 8812 SfcI C'TryA_G Cuts at: 826 1045 2994 3045 4743 5310 6074 6160 7295 Size: 219 1949 51 1698 567 764 86 1135 Cuts at: 7295 7976 8167 826 Size: 681 191 1471 Fragments arranged by size: 1949 1698 1471 1135 764 681 567 219 191 86 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8812 SmaI CCC'GGG Cuts at: 1872 6139 1872 Size: 4267 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4005 6916 7817 8079 820 Size: 197 926 2062 2911 901 262 1553 Fragments arranged by size: 2911 2062 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6603 493 Size: 6110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8812 SphI G_CATG'C Cuts at: 1132 3307 4511 6170 1132 Size: 2175 1204 1659 3774 Fragments arranged by size: 3774 2175 1659 1204 SspI AAT'ATT Cuts at: 5 52 4352 4600 4798 6736 5 Size: 47 4300 248 198 1938 2081 Fragments arranged by size: 4300 2081 1938 248 198 47 StuI AGG'CCT Cuts at: 1517 2566 1517 Size: 1049 7763 StyI C'CwwG_G Cuts at: 513 1065 2004 2478 3615 5391 5755 6251 513 Size: 552 939 474 1137 1776 364 496 3074 Fragments arranged by size: 3074 1776 1137 939 552 496 474 364 SwaI ATTT'AAAT Cuts at: 4470 4470 Size: 8812 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5790 6839 6856 7009 7194 7639 325 Size: 1255 4210 1049 17 153 185 445 1498 Fragments arranged by size: 4210 1498 1255 1049 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3809 4037 4330 Size: 274 80 33 51 1443 1832 228 293 Cuts at: 4330 4488 4983 5659 5770 7058 8770 96 Size: 158 495 676 111 1288 1712 138 Fragments arranged by size: 1832 1712 1443 1288 676 495 293 274 228 158 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8772 8804 8772 Size: 32 8780 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8812 XbaI T'CTAG_A Cuts at: 6148 6148 Size: 8812 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4946 4946 Size: 8812 XhoI C'TCGA_G Cuts at: 1943 4005 1943 Size: 2062 6750 XmaI C'CCGG_G Cuts at: 1870 6137 1870 Size: 4267 4545 XmnI GAAnn'nnTTC Cuts at: 2347 3207 3970 5931 6941 2347 Size: 860 763 1961 1010 4218 Fragments arranged by size: 4218 1961 1010 860 763 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6620 276 Size: 53 83 186 936 5086 2468 Fragments arranged by size: 5086 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FspAI KasI NarI NruI PacI PspOMI PspXI RsrII SacI SanDI SfiI SfoI SrfI