Restriction map for
pFN21AE2471
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII BalI BbeI BbsI
BciVI BclI BfrI BglI BglII
BpuAI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eco47III
Eco81I EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HindIII HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 1729 GACGTC GACGT^C
AccII 1621 CGCG CG^CG
AcsI 598 GAATTT [GA]^AATT[CT]
AcyI 1729 GACGTC G[GA]^CG[CT]C
AgeI 944 ACCGGT A^CCGGT
AhdI 1198 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 598 GAATTT [GA]^AATT[CT]
Asp700I 1694 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1198 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 929 CTCGGG C^[CT]CG[GA]G
AvrII 1434 CCTAGG C^CTAGG
BamHI 130 GGATCC G^GATCC
BanI 550 GGCACC G^G[CT][GA]CC
BanII 1068 GAGCTC G[GA]GC[CT]^C
BbrPI 892 CACGTG CAC^GTG
BbvCI 1647 CCTCAGC CCTCAGC(-5/-2)
BcnI 1056 CCCGG CC^[GC]GG
BlnI 1434 CCTAGG C^CTAGG
BsaAI 892 CACGTG [CT]AC^GT[GA]
BsaHI 1729 GACGTC G[GA]^CG[CT]C
BsaWI 944 ACCGGT [AT]^CCGG[AT]
BsmBI 1576 GAGACG CGTCTC(1/5)
BsmI 855 GAATGC GAATGC(1/-1)
BsoBI 929 CTCGGG C^[CT]CG[GA]G
BspMI 1598 ACCTGC ACCTGC(4/8)
BsrFI 944 ACCGGT [GA]^CCGG[CT]
BstAPI 1593 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1621 CGCG CG^CG
Cfr10I 944 ACCGGT [GA]^CCGG[CT]
DraII 1595 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1054 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1198 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1667 CCTCCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1595 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1501 GATATC GAT^ATC
Hin1I 1729 GACGTC G[GA]^CG[CT]C
HincII 1015 GTCAAC GT[CT]^[GA]AC
HindII 1015 GTCAAC GT[CT]^[GA]AC
HinfI 142 GACTC G^A[ACGT]TC
MfeI 503 CAATTG C^AATTG
MslI 1467 CATGTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 503 CAATTG C^AATTG
MvnI 1621 CGCG CG^CG
NciI 1056 CCCGG CC^[GC]GG
NspI 836 GCATGT [GA]CATG^[CT]
PflMI 1053 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 944 ACCGGT A^CCGGT
PleI 142 GACTC GAGTC(4/5)
PmaCI 892 CACGTG CAC^GTG
PmlI 892 CACGTG CAC^GTG
PpuMI 1595 AGGACCT [GA]G^G[AT]CC[CT]
SacI 1068 GAGCTC GAGCT^C
SapI 1365 GCTCTTC GCTCTTC(1/4)
SmlI 1065 CTTGAG C^T[CT][GA]AG
Van91I 1053 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1694 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 1594 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1766 CAGAGCCTG
BcgI 48 CGAGGATTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1583 CGAAGCTCCTGC
BlpI 1266 GCTGAGC GC^T[ACGT]AGC
1512 GCTCAGC
Bpu1102I 1266 GCTGAGC GC^T[ACGT]AGC
1512 GCTCAGC
BsaI 1048 GAGACC GGTCTC(1/5)
1349 GAGACC
BsgI 1600 CTGCAC GTGCAG(16/14)
1255 GTGCAG
BsiEI 1022 CGGCCG CG[GA][CT]^CG
1633 CGGCCG
BsrDI 634 GCAATG GCAATG(2/0)
734 GCAATG
CelII 1266 GCTGAGC GC^T[ACGT]AGC
1512 GCTCAGC
DsaI 471 CCATGG C^C[GA][CT]GG
539 CCATGG
EagI 1022 CGGCCG C^GGCCG
1633 CGGCCG
EclXI 1022 CGGCCG C^GGCCG
1633 CGGCCG
Eco52I 1022 CGGCCG C^GGCCG
1633 CGGCCG
HaeII 35 GGCGCT [GA]GCGC^[CT]
900 GGCGCT
HgaI 1033 GACGC GACGC(5/10)
1362 GACGC
MseI 602 TTAA T^TAA
701 TTAA
NcoI 471 CCATGG C^CATGG
539 CCATGG
SbfI 1272 CCTGCAGG CCTGCA^GG
1590 CCTGCAGG
Sse8387I 1272 CCTGCAGG CCTGCA^GG
1590 CCTGCAGG
TaqI 1171 TCGA T^CGA
1471 TCGA
Tru9I 602 TTAA T^TAA
701 TTAA
TthHB8I 1171 TCGA T^CGA
1471 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I Tsp509I TspRI XhoII