(Circular) (Six-base) MAPSORT of: pFN21AE2471.seq Check: 2241 from: 1 to: 6496 pFN21AE2471 6496 bp With 182 enzymes: SgfI * May 21, 2013 17:11 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3738 4306 278 Size: 53 83 186 936 2202 568 2468 Fragments arranged by size: 2468 2202 936 568 186 83 53 AccI GT'mk_AC Cuts at: 1937 3839 1937 Size: 1902 4594 Acc65I G'GTAC_C Cuts at: 3817 3817 Size: 6496 AclI AA'CG_TT Cuts at: 1563 4623 4996 1563 Size: 3060 373 3063 Fragments arranged by size: 3063 3060 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 2444 2793 2874 3002 3401 3746 4559 Size: 488 324 349 81 128 399 345 813 Cuts at: 4559 5943 6211 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 813 488 399 349 345 324 268 128 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6299 AflIII A'CryG_T Cuts at: 1203 5395 6372 1203 Size: 4192 977 1327 Fragments arranged by size: 4192 1327 977 AgeI A'CCGG_T Cuts at: 2949 5327 2949 Size: 2378 4118 AhdI GACnn_n'nnGTC Cuts at: 3208 5225 3208 Size: 2017 4479 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6496 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6496 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2433 2465 2433 Size: 32 6464 AlwNI CAG_nnn'CTG Cuts at: 3604 3776 4239 5811 3604 Size: 172 463 1572 4289 Fragments arranged by size: 4289 1572 463 172 ApaLI G'TGCA_C Cuts at: 4553 5709 4553 Size: 1156 5340 ApoI r'AATT_y Cuts at: 1493 2603 3805 4031 4183 6292 1493 Size: 1110 1202 226 152 2109 1697 Fragments arranged by size: 2109 1697 1202 1110 226 152 AseI AT'TA_AT Cuts at: 160 1032 5050 160 Size: 872 4018 1606 Fragments arranged by size: 4018 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 AvaI C'yCGr_G Cuts at: 1870 1943 2934 3821 1870 Size: 73 991 887 4545 Fragments arranged by size: 4545 991 887 73 AvrII C'CTAG_G Cuts at: 3439 3439 Size: 6496 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2739 2772 2739 Size: 33 6463 BamHI G'GATC_C Cuts at: 2135 3826 4193 2135 Size: 1691 367 4438 Fragments arranged by size: 4438 1691 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2555 3817 5272 618 Size: 325 217 627 768 1262 1455 1842 Fragments arranged by size: 1842 1455 1262 768 627 325 217 BanII G_rGCy'C Cuts at: 1391 3077 3815 1391 Size: 1686 738 4072 Fragments arranged by size: 4072 1686 738 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5525 BbvCI CC'TCA_GC Cuts at: 3653 3653 Size: 6496 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2042 2076 3577 3611 4687 4721 1903 Size: 34 105 34 1501 34 1076 34 3678 Fragments arranged by size: 3678 1501 1076 105 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4388 5598 4388 Size: 1210 5286 BclI T'GATC_A Cuts at: 1548 1548 Size: 6496 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5107 136 Size: 107 122 71 631 605 3435 1525 Fragments arranged by size: 3435 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6491 6491 Size: 6496 BlpI GC'TnA_GC Cuts at: 3272 3518 3913 3272 Size: 246 395 5855 Fragments arranged by size: 5855 395 246 Bme1580I G_kGCm'C Cuts at: 1790 4557 5713 1790 Size: 2767 1156 2573 Fragments arranged by size: 2767 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6496 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4227 5185 442 Size: 416 53 83 399 24 2810 958 1753 Fragments arranged by size: 2810 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6496 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2059 2059 Size: 6496 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2134 3067 3139 3396 3485 3753 5156 Size: 857 38 933 72 257 89 268 1403 Cuts at: 5156 1239 Size: 2579 Fragments arranged by size: 2579 1403 933 857 268 257 89 72 38 Bpu10I CC'TnA_GC Cuts at: 3653 3653 Size: 6496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3091 4621 5486 5784 3091 Size: 1530 865 298 3803 Fragments arranged by size: 3803 1530 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3047 3348 5159 882 Size: 617 1548 301 1811 2219 Fragments arranged by size: 2219 1811 1548 617 301 BsaAI yAC'GTr Cuts at: 493 1204 2899 4287 493 Size: 711 1695 1388 2702 Fragments arranged by size: 2702 1695 1388 711 BsaBI GATnn'nnATC Cuts at: 6490 6490 Size: 6496 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3735 4303 4685 5320 Size: 53 83 186 936 2202 568 382 635 Cuts at: 5320 275 Size: 1451 Fragments arranged by size: 2202 1451 936 635 568 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2949 4929 5327 5601 5748 6159 2949 Size: 1980 398 274 147 411 3286 Fragments arranged by size: 3286 1980 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2433 2463 3458 3488 3641 Size: 30 165 30 1030 30 995 30 153 Cuts at: 3641 3671 1178 Size: 30 4003 Fragments arranged by size: 4003 1030 995 165 153 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2906 3062 3065 1186 Size: 1720 156 3 4617 Fragments arranged by size: 4617 1720 156 3 BseYI C'CCAG_C Cuts at: 3112 3299 5699 6195 3112 Size: 187 2400 496 3413 Fragments arranged by size: 3413 2400 496 187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3281 3590 1163 Size: 573 1545 309 4069 Fragments arranged by size: 4069 1545 573 309 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3030 3641 3946 4707 5735 1540 Size: 275 186 1029 611 305 761 1028 2301 Fragments arranged by size: 2301 1029 1028 761 611 305 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 2420 2898 2919 3077 4557 4642 5713 2304 Size: 116 478 21 158 1480 85 1071 3087 Fragments arranged by size: 3087 1480 1071 478 158 116 85 21 BsmI GAATG_Cn' Cuts at: 2866 4007 4100 2866 Size: 1141 93 5262 Fragments arranged by size: 5262 1141 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3575 3575 Size: 6496 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2420 2898 2919 3077 3815 4557 Size: 399 514 116 478 21 158 738 742 Cuts at: 4557 4642 5713 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 738 514 478 399 158 116 85 21 BspEI T'CCGG_A Cuts at: 6159 6159 Size: 6496 BspHI T'CATG_A Cuts at: 4385 6115 4385 Size: 1730 4766 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3612 3851 844 Size: 2768 239 3489 Fragments arranged by size: 3489 2768 239 BsrBI CCG'CTC Cuts at: 1394 1823 4383 1394 Size: 429 2560 3507 Fragments arranged by size: 3507 2560 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2646 2746 4991 5165 65 Size: 1183 1398 100 2245 174 1396 Fragments arranged by size: 2245 1398 1396 1183 174 100 BsrFI r'CCGG_y Cuts at: 1700 1810 2949 5140 5327 1700 Size: 110 1139 2191 187 2869 Fragments arranged by size: 2869 2191 1139 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6496 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6496 BssSI C'ACGA_G Cuts at: 4556 5568 4556 Size: 1012 5484 BstAPI GCAn_nnn'nTGC Cuts at: 794 3604 794 Size: 2810 3686 BstBI TT'CG_AA Cuts at: 5300 5300 Size: 6496 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6496 BstYI r'GATC_y Cuts at: 1972 2135 2470 2688 3826 4193 4578 4595 6047 Size: 163 335 218 1138 367 385 17 1452 Cuts at: 6047 6133 6145 6491 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1138 385 367 346 335 218 163 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2476 2544 513 Size: 552 939 472 68 4465 Fragments arranged by size: 4465 939 552 472 68 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5757 Fragments arranged by size: 5757 683 56 BtsI GCAGTG_nn' Cuts at: 3269 4012 4806 4826 3269 Size: 743 794 20 4939 Fragments arranged by size: 4939 794 743 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6496 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6461 DraI TTT'AAA Cuts at: 3800 3800 Size: 6496 DraIII CAC_nnn'GTG Cuts at: 3064 3064 Size: 6496 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5503 809 Size: 507 4187 1802 Fragments arranged by size: 4187 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2140 2375 3027 Size: 54 1425 50 275 18 310 235 652 Cuts at: 3027 3638 3943 4832 6461 8 Size: 611 305 889 1629 43 Fragments arranged by size: 1629 1425 889 652 611 310 305 275 235 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3027 3638 3943 1537 Size: 275 1215 611 305 4090 Fragments arranged by size: 4090 1215 611 305 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2268 2978 3377 4426 1353 Size: 915 710 399 1049 3423 Fragments arranged by size: 3423 1049 915 710 399 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2441 3366 3635 5083 5457 5603 Size: 1064 138 852 925 269 1448 374 146 Cuts at: 5603 387 Size: 1280 Fragments arranged by size: 1448 1280 1064 925 852 374 269 146 138 EcoICRI GAG'CTC Cuts at: 3075 3075 Size: 6496 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2120 2134 2444 2793 2874 3002 Size: 393 464 24 14 310 349 81 128 Cuts at: 3002 3067 3139 3396 3401 3485 3746 3753 4559 Size: 65 72 257 5 84 261 7 806 Cuts at: 4559 5156 5943 6211 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 806 787 597 464 393 349 310 268 261 257 128 84 81 72 65 24 14 7 5 EcoNI CCTnn'n_nnAGG Cuts at: 3676 3676 Size: 6496 EcoO109I rG'GnC_Cy Cuts at: 3601 3601 Size: 6496 EcoRI G'AATT_C Cuts at: 3805 4183 3805 Size: 378 6118 EcoRV GAT'ATC Cuts at: 3508 3508 Size: 6496 FspI TGC'GCA Cuts at: 5002 5002 Size: 6496 HaeII r_GCGC'y Cuts at: 1678 2044 2909 5643 1678 Size: 366 865 2734 2531 Fragments arranged by size: 2734 2531 865 366 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2185 2455 2487 2817 Size: 32 135 32 581 32 270 32 330 Cuts at: 2817 2849 2962 2994 3059 3091 3194 3226 3458 Size: 32 113 32 65 32 103 32 232 Cuts at: 3458 3490 5137 5169 5211 5243 1373 Size: 32 1647 32 42 32 2626 Fragments arranged by size: 2626 1647 581 330 270 232 135 113 103 65 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3022 3840 4086 669 Size: 988 281 1084 818 246 3079 Fragments arranged by size: 3079 1084 988 818 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6496 HpaI GTT'AAC Cuts at: 4086 4086 Size: 6496 KpnI G_GTAC'C Cuts at: 3821 3821 Size: 6496 MfeI C'AATT_G Cuts at: 2508 4095 2508 Size: 1587 4909 MluI A'CGCG_T Cuts at: 6372 6372 Size: 6496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2086 3192 3672 3690 5610 5794 1305 Size: 781 1106 480 18 1920 184 2007 Fragments arranged by size: 2007 1920 1106 781 480 184 18 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4674 Fragments arranged by size: 4674 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3476 4454 4813 4972 518 Size: 648 2310 978 359 159 2042 Fragments arranged by size: 2310 2042 978 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2753 2838 2924 3379 3912 4589 5737 5982 Size: 1147 85 86 455 533 677 1148 245 Cuts at: 5982 1606 Size: 2120 Fragments arranged by size: 2120 1148 1147 677 533 455 245 86 85 NcoI C'CATG_G Cuts at: 513 1065 2004 2476 2544 513 Size: 552 939 472 68 4465 Fragments arranged by size: 4465 939 552 472 68 NdeI CA'TA_TG Cuts at: 387 387 Size: 6496 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6496 NotI GC'GGCC_GC Cuts at: 3943 3943 Size: 6496 NspI r_CATG'y Cuts at: 1132 1229 2845 3854 5399 1132 Size: 97 1616 1009 1545 2229 Fragments arranged by size: 2229 1616 1545 1009 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6496 PciI A'CATG_T Cuts at: 5395 5395 Size: 6496 PflMI CCAn_nnn'nTGG Cuts at: 1108 3064 1108 Size: 1956 4540 PfoI T'CCnGG_A Cuts at: 2436 2515 3333 2436 Size: 79 818 5599 Fragments arranged by size: 5599 818 79 PmeI GTTT'AAAC Cuts at: 3800 3800 Size: 6496 PmlI CAC'GTG Cuts at: 2899 2899 Size: 6496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2433 2465 4565 4597 6078 6110 2433 Size: 32 2100 32 1481 32 2819 Fragments arranged by size: 2819 2100 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3601 3601 Size: 6496 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6496 PsiI TTA'TAA Cuts at: 4066 4066 Size: 6496 PstI C_TGCA'G Cuts at: 830 2870 3282 3600 3848 830 Size: 2040 412 318 248 3478 Fragments arranged by size: 3478 2040 412 318 248 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6496 PvuII CAG'CTG Cuts at: 2753 2838 2924 3379 2753 Size: 85 86 455 5870 Fragments arranged by size: 5870 455 86 85 SacI G_AGCT'C Cuts at: 3077 3077 Size: 6496 SalI G'TCGA_C Cuts at: 1936 3838 1936 Size: 1902 4594 SapI GCTCTTCn'nnn_ Cuts at: 3377 3377 Size: 6496 SbfI CC_TGCA'GG Cuts at: 3282 3600 3848 3282 Size: 318 248 5930 Fragments arranged by size: 5930 318 248 ScaI AGT'ACT Cuts at: 4744 6456 4744 Size: 1712 4784 SfcI C'TryA_G Cuts at: 826 1045 2866 3010 3278 3307 3596 3844 4979 Size: 219 1821 144 268 29 289 248 1135 Cuts at: 4979 5660 5851 826 Size: 681 191 1471 Fragments arranged by size: 1821 1471 1135 681 289 268 248 219 191 144 29 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6496 SmaI CCC'GGG Cuts at: 1872 3823 1872 Size: 1951 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3070 4600 5501 5763 820 Size: 197 926 1127 1530 901 262 1553 Fragments arranged by size: 1553 1530 1127 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4287 493 Size: 3794 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6496 SphI G_CATG'C Cuts at: 1132 3854 1132 Size: 2722 3774 SspI AAT'ATT Cuts at: 5 52 4420 5 Size: 47 4368 2081 Fragments arranged by size: 4368 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6496 StyI C'CwwG_G Cuts at: 513 1065 2004 2476 2544 3423 3439 3935 513 Size: 552 939 472 68 879 16 496 3074 Fragments arranged by size: 3074 939 879 552 496 472 68 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3755 4523 4540 4693 4878 5323 325 Size: 1255 2175 768 17 153 185 445 1498 Fragments arranged by size: 2175 1498 1255 768 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2667 2785 4742 Size: 274 80 33 51 1443 690 118 1957 Cuts at: 4742 6454 96 Size: 1712 138 Fragments arranged by size: 1957 1712 1443 690 274 138 118 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2103 2135 6456 6488 2103 Size: 32 4321 32 2111 Fragments arranged by size: 4321 2111 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6496 XbaI T'CTAG_A Cuts at: 3832 3832 Size: 6496 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6496 XmaI C'CCGG_G Cuts at: 1870 3821 1870 Size: 1951 4545 XmnI GAAnn'nnTTC Cuts at: 3703 4625 3703 Size: 922 5574 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3736 4304 276 Size: 53 83 186 936 2202 568 2468 Fragments arranged by size: 2468 2202 936 568 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI