(Circular) (Six-base) MAPSORT of: pFN21AB9932.seq Check: 1820 from: 1 to: 6619 pFN21AB9932 6619 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3208 3220 3208 Size: 12 6607 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4429 278 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3834 3962 1937 Size: 1897 128 4594 Fragments arranged by size: 4594 1897 128 Acc65I G'GTAC_C Cuts at: 3940 3940 Size: 6619 AclI AA'CG_TT Cuts at: 1563 4746 5119 1563 Size: 3183 373 3063 Fragments arranged by size: 3183 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2080 2597 4682 6066 6334 1632 Size: 448 517 2085 1384 268 1917 Fragments arranged by size: 2085 1917 1384 517 448 268 AflII C'TTAA_G Cuts at: 820 1017 3049 820 Size: 197 2032 4390 Fragments arranged by size: 4390 2032 197 AflIII A'CryG_T Cuts at: 1203 5518 6495 1203 Size: 4315 977 1327 Fragments arranged by size: 4315 1327 977 AgeI A'CCGG_T Cuts at: 5450 5450 Size: 6619 AhdI GACnn_n'nnGTC Cuts at: 5348 5348 Size: 6619 AleI CACnn'nnGTG Cuts at: 1166 3258 1166 Size: 2092 4527 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6619 AlwNI CAG_nnn'CTG Cuts at: 2294 4362 5934 2294 Size: 2068 1572 2979 Fragments arranged by size: 2979 2068 1572 ApaLI G'TGCA_C Cuts at: 2155 2425 4676 5832 2155 Size: 270 2251 1156 2942 Fragments arranged by size: 2942 2251 1156 270 ApoI r'AATT_y Cuts at: 1493 2889 3494 3928 4154 4306 6415 1493 Size: 1396 605 434 226 152 2109 1697 Fragments arranged by size: 2109 1697 1396 605 434 226 152 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 2896 3944 1870 Size: 73 953 1048 4545 Fragments arranged by size: 4545 1048 953 73 BamHI G'GATC_C Cuts at: 3949 4316 3949 Size: 367 6252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3252 3940 5395 618 Size: 325 217 627 1465 688 1455 1842 Fragments arranged by size: 1842 1465 1455 688 627 325 217 BanII G_rGCy'C Cuts at: 1391 3107 3938 1391 Size: 1716 831 4072 Fragments arranged by size: 4072 1716 831 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2765 3024 928 Size: 971 866 259 4523 Fragments arranged by size: 4523 971 866 259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4810 4844 1903 Size: 34 2873 34 3678 Fragments arranged by size: 3678 2873 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4511 5721 4511 Size: 1210 5409 BclI T'GATC_A Cuts at: 1548 1548 Size: 6619 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5230 136 Size: 107 122 71 631 605 3558 1525 Fragments arranged by size: 3558 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6614 6614 Size: 6619 BlpI GC'TnA_GC Cuts at: 4036 4036 Size: 6619 Bme1580I G_kGCm'C Cuts at: 1790 2159 2429 2590 3814 4680 5836 1790 Size: 369 270 161 1224 866 1156 2573 Fragments arranged by size: 2573 1224 1156 866 369 270 161 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6619 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3144 4350 5308 Size: 416 53 83 399 24 1727 1206 958 Cuts at: 5308 442 Size: 1753 Fragments arranged by size: 1753 1727 1206 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6619 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3287 3287 Size: 6619 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2652 3455 5279 1239 Size: 1413 803 1824 2579 Fragments arranged by size: 2579 1824 1413 803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2802 4744 5609 5907 2802 Size: 1942 865 298 3514 Fragments arranged by size: 3514 1942 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5282 882 Size: 617 3783 2219 Fragments arranged by size: 3783 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4410 493 Size: 711 3206 2702 Fragments arranged by size: 3206 2702 711 BsaBI GATnn'nnATC Cuts at: 6613 6613 Size: 6619 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4426 4808 5443 275 Size: 53 83 186 936 2893 382 635 1451 Fragments arranged by size: 2893 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2490 3563 5052 5450 5724 5871 6282 2490 Size: 1073 1489 398 274 147 411 2827 Fragments arranged by size: 2827 1489 1073 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2210 2240 2937 2967 3094 Size: 30 165 30 807 30 697 30 127 Cuts at: 3094 3124 1178 Size: 30 4673 Fragments arranged by size: 4673 807 697 165 127 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2385 2805 2928 1186 Size: 1199 420 123 4877 Fragments arranged by size: 4877 1199 420 123 BseYI C'CCAG_C Cuts at: 3795 5822 6318 3795 Size: 2027 496 4096 Fragments arranged by size: 4096 2027 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2307 2680 3136 1163 Size: 573 571 373 456 4646 Fragments arranged by size: 4646 573 571 456 373 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4069 4830 5858 1540 Size: 275 186 2068 761 1028 2301 Fragments arranged by size: 2301 2068 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2159 2429 3107 4680 4765 5836 2159 Size: 270 678 1573 85 1071 2942 Fragments arranged by size: 2942 1573 1071 678 270 85 BsmI GAATG_Cn' Cuts at: 2417 3650 4130 4223 2417 Size: 1233 480 93 4813 Fragments arranged by size: 4813 1233 480 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2429 2590 3107 3814 3938 4680 Size: 399 369 270 161 517 707 124 742 Cuts at: 4680 4765 5836 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 707 517 399 369 270 161 124 85 BspEI T'CCGG_A Cuts at: 6282 6282 Size: 6619 BspHI T'CATG_A Cuts at: 3448 4508 6238 3448 Size: 1060 1730 3829 Fragments arranged by size: 3829 1730 1060 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2861 3208 3220 3974 844 Size: 2017 347 12 754 3489 Fragments arranged by size: 3489 2017 754 347 12 BsrBI CCG'CTC Cuts at: 1394 1823 4506 1394 Size: 429 2683 3507 Fragments arranged by size: 3507 2683 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2318 5114 5288 65 Size: 1183 1070 2796 174 1396 Fragments arranged by size: 2796 1396 1183 1070 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5263 5450 1700 Size: 110 3453 187 2869 Fragments arranged by size: 3453 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2121 96 Size: 2025 4594 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6619 BssSI C'ACGA_G Cuts at: 4679 5691 4679 Size: 1012 5607 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6619 BstBI TT'CG_AA Cuts at: 5423 5423 Size: 6619 BstEII G'GTnAC_C Cuts at: 1184 3559 1184 Size: 2375 4244 BstYI r'GATC_y Cuts at: 1972 3949 4316 4701 4718 6170 6256 6268 6614 Size: 1977 367 385 17 1452 86 12 346 Cuts at: 6614 1972 Size: 1977 Fragments arranged by size: 1977 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6067 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5880 Fragments arranged by size: 5880 683 56 BtsI GCAGTG_nn' Cuts at: 2407 3294 3304 3892 4135 4929 4949 2407 Size: 887 10 588 243 794 20 4077 Fragments arranged by size: 4077 887 794 588 243 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6584 DraI TTT'AAA Cuts at: 3360 3923 3360 Size: 563 6056 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3146 5626 809 Size: 507 1830 2480 1802 Fragments arranged by size: 2480 1830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4066 4955 6584 Size: 54 1425 50 275 18 2236 889 1629 Cuts at: 6584 8 Size: 43 Fragments arranged by size: 2236 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4066 1537 Size: 275 2254 4090 Fragments arranged by size: 4090 2254 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2723 3024 3199 4549 1353 Size: 1370 301 175 1350 3423 Fragments arranged by size: 3423 1370 1350 301 175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5206 5580 5726 387 Size: 1064 138 3617 374 146 1280 Fragments arranged by size: 3617 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3105 3105 Size: 6619 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2597 2652 3455 4682 5279 6066 Size: 393 448 517 55 803 1227 597 787 Cuts at: 6066 6334 1239 Size: 268 1524 Fragments arranged by size: 1524 1227 803 787 597 517 448 393 268 55 EcoNI CCTnn'n_nnAGG Cuts at: 3522 3522 Size: 6619 EcoO109I rG'GnC_Cy Cuts at: 3289 3289 Size: 6619 EcoRI G'AATT_C Cuts at: 3494 3928 4306 3494 Size: 434 378 5807 Fragments arranged by size: 5807 434 378 EcoRV GAT'ATC Cuts at: 3454 3659 3454 Size: 205 6414 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3064 3064 Size: 6619 FspI TGC'GCA Cuts at: 5125 5125 Size: 6619 HaeII r_GCGC'y Cuts at: 1678 5766 1678 Size: 4088 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2971 3003 5260 5292 5334 Size: 32 135 32 1399 32 2257 32 42 Cuts at: 5334 5366 1373 Size: 32 2626 Fragments arranged by size: 2626 2257 1399 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2905 3963 4209 669 Size: 988 281 967 1058 246 3079 Fragments arranged by size: 3079 1058 988 967 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6619 HpaI GTT'AAC Cuts at: 4209 4209 Size: 6619 KpnI G_GTAC'C Cuts at: 3944 3944 Size: 6619 MfeI C'AATT_G Cuts at: 2497 4218 2497 Size: 1721 4898 MluI A'CGCG_T Cuts at: 6495 6495 Size: 6619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2666 3098 3160 5733 5917 1305 Size: 1361 432 62 2573 184 2007 Fragments arranged by size: 2573 2007 1361 432 184 62 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4797 Fragments arranged by size: 4797 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2975 3258 4577 4936 5095 518 Size: 648 1809 283 1319 359 159 2042 Fragments arranged by size: 2042 1809 1319 648 359 283 159 MspA1I CmG'CkG Cuts at: 1606 3795 4035 4712 5860 6105 1606 Size: 2189 240 677 1148 245 2120 Fragments arranged by size: 2189 2120 1148 677 245 240 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6067 NdeI CA'TA_TG Cuts at: 387 2458 387 Size: 2071 4548 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6619 NotI GC'GGCC_GC Cuts at: 4066 4066 Size: 6619 NspI r_CATG'y Cuts at: 1132 1229 3977 5522 1132 Size: 97 2748 1545 2229 Fragments arranged by size: 2748 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6619 PciI A'CATG_T Cuts at: 5518 5518 Size: 6619 PflMI CCAn_nnn'nTGG Cuts at: 1108 3555 3685 1108 Size: 2447 130 4042 Fragments arranged by size: 4042 2447 130 PfoI T'CCnGG_A Cuts at: 2518 2518 Size: 6619 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4688 4720 6201 6233 4688 Size: 32 1481 32 5074 Fragments arranged by size: 5074 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3289 3289 Size: 6619 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6619 PsiI TTA'TAA Cuts at: 2352 3669 4189 2352 Size: 1317 520 4782 Fragments arranged by size: 4782 1317 520 PstI C_TGCA'G Cuts at: 830 3231 3330 3971 830 Size: 2401 99 641 3478 Fragments arranged by size: 3478 2401 641 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6619 PvuII CAG'CTG Cuts at: 3795 3795 Size: 6619 SacI G_AGCT'C Cuts at: 3107 3107 Size: 6619 SalI G'TCGA_C Cuts at: 1936 3961 1936 Size: 2025 4594 SbfI CC_TGCA'GG Cuts at: 3231 3330 3971 3231 Size: 99 641 5879 Fragments arranged by size: 5879 641 99 ScaI AGT'ACT Cuts at: 4867 6579 4867 Size: 1712 4907 SexAI A'CCwGG_T Cuts at: 3826 3826 Size: 6619 SfcI C'TryA_G Cuts at: 826 1045 2049 3227 3326 3346 3967 5102 5783 Size: 219 1004 1178 99 20 621 1135 681 Cuts at: 5783 5974 826 Size: 191 1471 Fragments arranged by size: 1471 1178 1135 1004 681 621 219 191 99 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6619 SmaI CCC'GGG Cuts at: 1872 2898 3946 1872 Size: 1026 1048 4545 Fragments arranged by size: 4545 1048 1026 SmlI C'TyrA_G Cuts at: 820 1017 1943 2817 3049 4723 5624 5886 820 Size: 197 926 874 232 1674 901 262 1553 Fragments arranged by size: 1674 1553 926 901 874 262 232 197 SnaBI TAC'GTA Cuts at: 493 4410 493 Size: 3917 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 3977 1132 Size: 2845 3774 SspI AAT'ATT Cuts at: 5 52 3604 3622 4543 5 Size: 47 3552 18 921 2081 Fragments arranged by size: 3552 2081 921 47 18 StuI AGG'CCT Cuts at: 1517 1517 Size: 6619 StyI C'CwwG_G Cuts at: 513 1065 2168 4058 513 Size: 552 1103 1890 3074 Fragments arranged by size: 3074 1890 1103 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2181 4646 4663 4816 5001 5446 325 Size: 1255 601 2465 17 153 185 445 1498 Fragments arranged by size: 2465 1498 1255 601 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2121 2272 2511 Size: 274 80 33 51 1443 144 151 239 Cuts at: 2511 3852 4865 6577 96 Size: 1341 1013 1712 138 Fragments arranged by size: 1712 1443 1341 1013 274 239 151 144 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3020 3052 3676 3708 6579 6611 3020 Size: 32 624 32 2871 32 3028 Fragments arranged by size: 3028 2871 624 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6619 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3311 3311 Size: 6619 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6619 XmaI C'CCGG_G Cuts at: 1870 2896 3944 1870 Size: 1026 1048 4545 Fragments arranged by size: 4545 1048 1026 XmnI GAAnn'nnTTC Cuts at: 2618 4748 2618 Size: 2130 4489 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4427 276 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI