################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsiEI BsiWI BsmFI BspDI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV FbaI FseI FspI HaeII HhaI HinP1I HpaI KasI KpnI KspI MaeI MamI MflI MluI MluNI MscI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 1045 GTCTAC GT^[AC][GT]AC AlwI 1549 GATCC GGATC(4/5) AlwNI 392 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 318 CCTGAGG CC^T[ACGT]AGG AseI 870 ATTAAT AT^TAAT AsnI 870 ATTAAT AT^TAAT AvaI 752 CTCGGG C^[CT]CG[GA]G BanI 998 GGCACC G^G[CT][GA]CC BbvCI 894 GCTGAGG CCTCAGC(-5/-2) BseRI 1308 GAGGAG GAGGAG(10/8) BsmBI 4 GAGACG CGTCTC(1/5) BsoBI 752 CTCGGG C^[CT]CG[GA]G Bsp1407I 375 TGTACA T^GTACA BspMI 1465 GCAGGT ACCTGC(4/8) BsrDI 832 GCAATG GCAATG(2/0) BsrGI 375 TGTACA T^GTACA BstAPI 803 GCAGTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 737 GGTTACC G^GT[ACGT]ACC BstNI 1451 CCAGG CC^[AT]GG BstPI 737 GGTTACC G^GT[ACGT]ACC BstXI 917 CCAAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 318 CCTGAGG CC^T[ACGT]AGG DpnII 1549 GATC ^GATC DraI 982 TTTAAA TTT^AAA DrdI 1710 GACAGTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 221 TGGCCG [CT]^GGCC[GA] Eco81I 318 CCTGAGG CC^T[ACGT]AGG EcoO65I 737 GGTTACC G^GT[ACGT]ACC EcoRII 1451 CCAGG ^CC[AT]GG EcoT14I 106 CCTTGG C^C[AT][AT]GG FauI 22 CCCGC CCCGC(4/6) HincII 466 GTCAAC GT[CT]^[GA]AC HindII 466 GTCAAC GT[CT]^[GA]AC HindIII 122 AAGCTT A^AGCTT MaeII 1377 ACGT A^CGT MboI 1549 GATC ^GATC MfeI 176 CAATTG C^AATTG MunI 176 CAATTG C^AATTG MvaI 1451 CCAGG CC^[AT]GG NdeI 112 CATATG CA^TATG NdeII 1549 GATC ^GATC PflMI 391 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 870 ATTAAT AT^TAAT PstI 347 CTGCAG CTGCA^G PvuII 395 CAGCTG CAG^CTG Sau3AI 1549 GATC ^GATC SmlI 618 CTCAAG C^T[CT][GA]AG SspBI 375 TGTACA T^GTACA StyI 106 CCTTGG C^C[AT][AT]GG Van91I 391 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 917 CCAAATTGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 884 TCCGGA T^CCGGA 959 TCCGGA AcyI 6 GACGCC G[GA]^CG[CT]C 1671 GGCGTC AflIII 729 ACATGT A^C[GA][CT]GT 1589 ACATGT Asp700I 120 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 574 GAAGAATTTC AspHI 1446 GAGCAC G[AT]GC[AT]^C 1731 GAGCAC BbsI 187 GAAGAC GAAGAC(2/6) 426 GAAGAC BmyI 1446 GAGCAC G[AGT]GC[ACT]^C 1731 GAGCAC BpuAI 187 GAAGAC GAAGAC(2/6) 426 GAAGAC BsaHI 6 GACGCC G[GA]^CG[CT]C 1671 GGCGTC BsaI 1160 GAGACC GGTCTC(1/5) 400 GGTCTC BsaJI 106 CCTTGG C^C[ACGT][ACGT]GG 751 CCTCGG BseAI 884 TCCGGA T^CCGGA 959 TCCGGA BsiHKAI 1446 GAGCAC G[AT]GC[AT]^C 1731 GAGCAC BsmI 970 GAATGC GAATGC(1/-1) 1399 GAATGC Bsp1286I 1446 GAGCAC G[AGT]GC[ACT]^C 1731 GAGCAC BspEI 884 TCCGGA T^CCGGA 959 TCCGGA BspHI 134 TCATGA T^CATGA 1509 TCATGA BspLU11I 729 ACATGT A^CATGT 1589 ACATGT BsrI 91 CCAGT ACTGG(1/-1) 1134 CCAGT EcoT22I 499 ATGCAT ATGCA^T 503 ATGCAT HgaI 6 GACGC GACGC(5/10) 1672 GCGTC Hin1I 6 GACGCC G[GA]^CG[CT]C 1671 GGCGTC MroI 884 TCCGGA T^CCGGA 959 TCCGGA MslI 174 CACAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1376 CACGTCAGTG MspA1I 13 CCGCTG C[AC]G^C[GT]G 395 CAGCTG NsiI 499 ATGCAT ATGCA^T 503 ATGCAT RcaI 134 TCATGA T^CATGA 1509 TCATGA SphI 25 GCATGC GCATG^C 501 GCATGC SspI 758 AATATT AAT^ATT 1682 AATATT TaqI 56 TCGA T^CGA 596 TCGA TthHB8I 56 TCGA T^CGA 596 TCGA XmnI 120 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 574 GAAGAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BbvI BcnI BsaWI BsgI BsiYI BslI BsmAI BssKI BstF5I Cac8I Cfr13I DdeI EarI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboII MnlI MseI MspI MwoI NciI NlaIII NlaIV NspI RsaI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI