(Circular) (Six-base) MAPSORT of: pFN21AE6489.seq Check: 9589 from: 1 to: 6484 pFN21AE6489 6484 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3461 3461 Size: 6484 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3051 3827 1937 Size: 1114 776 4594 Fragments arranged by size: 4594 1114 776 Acc65I G'GTAC_C Cuts at: 3805 3805 Size: 6484 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2631 4547 5931 6199 1632 Size: 999 1916 1384 268 1917 Fragments arranged by size: 1917 1916 1384 999 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 2734 3594 5383 6360 1203 Size: 1531 860 1789 977 1327 Fragments arranged by size: 1789 1531 1327 977 860 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 5213 5213 Size: 6484 AleI CACnn'nnGTG Cuts at: 1166 2183 3385 1166 Size: 1017 1202 4265 Fragments arranged by size: 4265 1202 1017 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2574 1184 Size: 1390 5094 AlwNI CAG_nnn'CTG Cuts at: 2402 4227 5799 2402 Size: 1825 1572 3087 Fragments arranged by size: 3087 1825 1572 ApaLI G'TGCA_C Cuts at: 4541 5697 4541 Size: 1156 5328 ApoI r'AATT_y Cuts at: 1493 2161 2202 2358 2444 2582 3793 4019 4171 Size: 668 41 156 86 138 1211 226 152 Cuts at: 4171 6280 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1211 668 226 156 152 138 86 41 AseI AT'TA_AT Cuts at: 160 1032 2876 5038 160 Size: 872 1844 2162 1606 Fragments arranged by size: 2162 1844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 2757 3809 1870 Size: 73 814 1052 4545 Fragments arranged by size: 4545 1052 814 73 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3003 3805 5260 618 Size: 325 217 627 1216 802 1455 1842 Fragments arranged by size: 1842 1455 1216 802 627 325 217 BanII G_rGCy'C Cuts at: 1391 3803 1391 Size: 2412 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2199 2438 928 Size: 971 300 239 4974 Fragments arranged by size: 4974 971 300 239 BbvCI CC'TCA_GC Cuts at: 2900 2900 Size: 6484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4675 4709 1903 Size: 34 2738 34 3678 Fragments arranged by size: 3678 2738 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 1548 Size: 6484 BfrBI ATG'CAT Cuts at: 2506 2510 2506 Size: 4 6480 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5095 136 Size: 107 122 71 631 605 3423 1525 Fragments arranged by size: 3423 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 3901 3901 Size: 6484 Bme1580I G_kGCm'C Cuts at: 1790 4545 5701 1790 Size: 2755 1156 2573 Fragments arranged by size: 2755 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3383 1318 Size: 2065 4419 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2090 4215 5173 Size: 416 53 83 399 24 673 2125 958 Cuts at: 5173 442 Size: 1753 Fragments arranged by size: 2125 1753 958 673 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3365 3365 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5144 1239 Size: 3905 2579 Bpu10I CC'TnA_GC Cuts at: 2479 2900 2479 Size: 421 6063 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2608 4609 5474 5772 2608 Size: 2001 865 298 3320 Fragments arranged by size: 3320 2001 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2411 3159 5147 882 Size: 617 912 748 1988 2219 Fragments arranged by size: 2219 1988 912 748 617 BsaAI yAC'GTr Cuts at: 493 1204 4275 493 Size: 711 3071 2702 Fragments arranged by size: 3071 2702 711 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3677 4291 4673 Size: 53 83 186 936 479 1665 614 382 Cuts at: 4673 5308 275 Size: 635 1451 Fragments arranged by size: 1665 1451 936 635 614 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2889 2964 3168 4917 5315 5589 5736 6147 2889 Size: 75 204 1749 398 274 147 411 3226 Fragments arranged by size: 3226 1749 411 398 274 204 147 75 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3177 3207 3226 3256 3304 Size: 30 165 30 1774 30 19 30 48 Cuts at: 3304 3334 3459 3489 1178 Size: 30 125 30 4173 Fragments arranged by size: 4173 1774 165 125 48 30 30 30 30 30 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3328 1186 Size: 2142 4342 BseYI C'CCAG_C Cuts at: 2395 2457 5687 6183 2395 Size: 62 3230 496 2696 Fragments arranged by size: 3230 2696 496 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2632 3055 3619 3642 1163 Size: 573 896 423 564 23 4005 Fragments arranged by size: 4005 896 573 564 423 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3934 4695 5723 1540 Size: 275 186 1933 761 1028 2301 Fragments arranged by size: 2301 1933 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3455 3740 4545 4630 5701 3455 Size: 285 805 85 1071 4238 Fragments arranged by size: 4238 1071 805 285 85 BsmI GAATG_Cn' Cuts at: 2981 3410 3995 4088 2981 Size: 429 585 93 5377 Fragments arranged by size: 5377 585 429 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 2003 Size: 6484 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3455 3740 3803 4545 4630 5701 1391 Size: 399 1665 285 63 742 85 1071 2174 Fragments arranged by size: 2174 1665 1071 742 399 285 85 63 BspEI T'CCGG_A Cuts at: 2889 2964 6147 2889 Size: 75 3183 3226 Fragments arranged by size: 3226 3183 75 BspHI T'CATG_A Cuts at: 2139 3514 4373 6103 2139 Size: 1375 859 1730 2520 Fragments arranged by size: 2520 1730 1375 859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3461 3839 844 Size: 2617 378 3489 Fragments arranged by size: 3489 2617 378 BsrBI CCG'CTC Cuts at: 1394 1823 4371 1394 Size: 429 2548 3507 Fragments arranged by size: 3507 2548 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2844 4979 5153 65 Size: 1183 1596 2135 174 1396 Fragments arranged by size: 2135 1596 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5128 5315 1700 Size: 110 3318 187 2869 Fragments arranged by size: 3318 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2380 96 Size: 2284 4200 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6484 BssSI C'ACGA_G Cuts at: 4544 5556 4544 Size: 1012 5472 BstAPI GCAn_nnn'nTGC Cuts at: 794 2814 794 Size: 2020 4464 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 2742 1184 Size: 1558 4926 BstXI CCAn_nnnn'nTGG Cuts at: 2929 2929 Size: 6484 BstYI r'GATC_y Cuts at: 1972 3814 4181 4566 4583 6035 6121 6133 6479 Size: 1842 367 385 17 1452 86 12 346 Cuts at: 6479 1972 Size: 1977 Fragments arranged by size: 1977 1842 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2324 2324 Size: 6484 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4993 Fragments arranged by size: 4993 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2187 487 Size: 683 56 961 4784 Fragments arranged by size: 4784 961 683 56 BtsI GCAGTG_nn' Cuts at: 2089 2349 2815 3325 4000 4794 4814 2089 Size: 260 466 510 675 794 20 3759 Fragments arranged by size: 3759 794 675 510 466 260 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 2989 3788 2989 Size: 799 5685 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3721 5491 809 Size: 507 2405 1770 1802 Fragments arranged by size: 2405 1802 1770 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 3931 4820 Size: 54 1425 50 275 18 396 1705 889 Cuts at: 4820 6449 8 Size: 1629 43 Fragments arranged by size: 1705 1629 1425 889 396 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3931 1537 Size: 275 2119 4090 Fragments arranged by size: 4090 2119 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2646 2778 2876 4414 1353 Size: 1293 132 98 1538 3423 Fragments arranged by size: 3423 1538 1293 132 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3475 5071 5445 5591 387 Size: 1064 138 1886 1596 374 146 1280 Fragments arranged by size: 1886 1596 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2631 4547 5144 5931 6199 1239 Size: 393 999 1916 597 787 268 1524 Fragments arranged by size: 1916 1524 999 787 597 393 268 EcoRI G'AATT_C Cuts at: 3793 4171 3793 Size: 378 6106 FspI TGC'GCA Cuts at: 4990 4990 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 5631 1678 Size: 3953 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2092 2124 3362 3394 5125 Size: 32 135 32 520 32 1238 32 1731 Cuts at: 5125 5157 5199 5231 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1731 1238 520 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2473 3828 4074 669 Size: 988 281 535 1355 246 3079 Fragments arranged by size: 3079 1355 988 535 281 246 HindIII A'AGCT_T Cuts at: 748 2127 748 Size: 1379 5105 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KpnI G_GTAC'C Cuts at: 3809 3809 Size: 6484 MfeI C'AATT_G Cuts at: 2181 4083 2181 Size: 1902 4582 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3319 3628 5598 5782 1305 Size: 2014 309 1970 184 2007 Fragments arranged by size: 2014 2007 1970 309 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4662 Fragments arranged by size: 4662 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2183 3385 4442 4801 4960 518 Size: 648 1017 1202 1057 359 159 2042 Fragments arranged by size: 2042 1202 1057 1017 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2020 2402 3900 4577 5725 5970 1606 Size: 414 382 1498 677 1148 245 2120 Fragments arranged by size: 2120 1498 1148 677 414 382 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4993 Fragments arranged by size: 4993 939 552 NdeI CA'TA_TG Cuts at: 387 2118 387 Size: 1731 4753 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NsiI A_TGCA'T Cuts at: 2508 2512 2508 Size: 4 6480 NspI r_CATG'y Cuts at: 1132 1229 2034 2510 2514 2738 3598 3842 5387 Size: 97 805 476 4 224 860 244 1545 Cuts at: 5387 1132 Size: 2229 Fragments arranged by size: 2229 1545 860 805 476 244 224 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6484 PciI A'CATG_T Cuts at: 2734 3594 5383 2734 Size: 860 1789 3835 Fragments arranged by size: 3835 1789 860 PflMI CCAn_nnn'nTGG Cuts at: 1108 2402 1108 Size: 1294 5190 PfoI T'CCnGG_A Cuts at: 3026 3026 Size: 6484 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 3693 4054 3693 Size: 361 6123 PstI C_TGCA'G Cuts at: 830 2356 3836 830 Size: 1526 1480 3478 Fragments arranged by size: 3478 1526 1480 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 2402 2402 Size: 6484 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SfcI C'TryA_G Cuts at: 826 1045 2233 2352 3111 3357 3504 3832 4967 Size: 219 1188 119 759 246 147 328 1135 Cuts at: 4967 5648 5839 826 Size: 681 191 1471 Fragments arranged by size: 1471 1188 1135 759 681 328 246 219 191 147 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3811 1872 Size: 1939 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2623 4588 5489 5751 820 Size: 197 926 680 1965 901 262 1553 Fragments arranged by size: 1965 1553 926 901 680 262 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 2034 2510 3842 1132 Size: 902 476 1332 3774 Fragments arranged by size: 3774 1332 902 476 SspI AAT'ATT Cuts at: 5 52 2765 3689 4408 5 Size: 47 2713 924 719 2081 Fragments arranged by size: 2713 2081 924 719 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6484 StyI C'CwwG_G Cuts at: 513 1065 2004 2111 3923 513 Size: 552 939 107 1812 3074 Fragments arranged by size: 3074 1812 939 552 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2671 2923 3320 4511 4528 4681 4866 Size: 1255 1091 252 397 1191 17 153 185 Cuts at: 4866 5311 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1191 1091 445 397 252 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2380 2569 3236 Size: 274 80 33 51 1443 403 189 667 Cuts at: 3236 4730 6442 96 Size: 1494 1712 138 Fragments arranged by size: 1712 1494 1443 667 403 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3177 3209 3304 3336 6444 6476 3177 Size: 32 95 32 3108 32 3185 Fragments arranged by size: 3185 3108 95 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6484 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2929 2929 Size: 6484 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 3809 1870 Size: 1939 4545 XmnI GAAnn'nnTTC Cuts at: 2129 2583 4613 2129 Size: 454 2030 4000 Fragments arranged by size: 4000 2030 454 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI