(Linear) (Six-base) MAPSORT of: KIEE6489.seq Check: 1872 from: 1 to: 1779 KIEE6489.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1456 1779 Size: 1456 323 AccI GT'mk_AC Cuts at: 0 1046 1779 Size: 1046 733 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 626 1779 Size: 626 1153 AflIII A'CryG_T Cuts at: 0 729 1589 1779 Size: 729 860 190 Fragments arranged by size: 860 729 190 AleI CACnn'nnGTG Cuts at: 0 178 1380 1779 Size: 178 1202 399 Fragments arranged by size: 1202 399 178 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 569 1779 Size: 569 1210 AlwNI CAG_nnn'CTG Cuts at: 0 397 1779 Size: 397 1382 ApoI r'AATT_y Cuts at: 0 156 197 353 439 577 1779 Size: 156 41 156 86 138 1202 Fragments arranged by size: 1202 156 156 138 86 41 AseI AT'TA_AT Cuts at: 0 871 1779 Size: 871 908 AvaI C'yCGr_G Cuts at: 0 752 1779 Size: 752 1027 BanI G'GyrC_C Cuts at: 0 998 1779 Size: 998 781 BbsI GAAGACnn'nnnn_ Cuts at: 0 194 433 1779 Size: 194 239 1346 Fragments arranged by size: 1346 239 194 BbvCI CC'TCA_GC Cuts at: 0 895 1779 Size: 895 884 BfrBI ATG'CAT Cuts at: 0 501 505 1779 Size: 501 4 1274 Fragments arranged by size: 1274 501 4 BmgBI CAC'GTC Cuts at: 0 1378 1779 Size: 1378 401 BmrI ACTGGGnnnn_n' Cuts at: 0 85 1779 Size: 85 1694 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1360 1779 Size: 1360 419 Bpu10I CC'TnA_GC Cuts at: 0 474 895 1779 Size: 474 421 884 Fragments arranged by size: 884 474 421 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 603 1779 Size: 603 1176 BsaI GGTCTCn'nnnn_ Cuts at: 0 406 1154 1779 Size: 406 748 625 Fragments arranged by size: 748 625 406 BsaHI Gr'CG_yC Cuts at: 0 7 1672 1779 Size: 7 1665 107 Fragments arranged by size: 1665 107 7 BsaWI w'CCGG_w Cuts at: 0 884 959 1163 1779 Size: 884 75 204 616 Fragments arranged by size: 884 616 204 75 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1172 1202 1221 1251 1299 1329 1454 1484 Size: 1172 30 19 30 48 30 125 30 Cuts at: 1484 1779 Size: 295 Fragments arranged by size: 1172 295 125 48 30 30 30 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1323 1779 Size: 1323 456 BseYI C'CCAG_C Cuts at: 0 390 452 1779 Size: 390 62 1327 Fragments arranged by size: 1327 390 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 627 1050 1614 1637 1779 Size: 627 423 564 23 142 Fragments arranged by size: 627 564 423 142 23 BsiHKAI G_wGCw'C Cuts at: 0 1450 1735 1779 Size: 1450 285 44 Fragments arranged by size: 1450 285 44 BsmI GAATG_Cn' Cuts at: 0 976 1405 1779 Size: 976 429 374 Fragments arranged by size: 976 429 374 Bsp1286I G_dGCh'C Cuts at: 0 1450 1735 1779 Size: 1450 285 44 Fragments arranged by size: 1450 285 44 BspEI T'CCGG_A Cuts at: 0 884 959 1779 Size: 884 75 820 Fragments arranged by size: 884 820 75 BspHI T'CATG_A Cuts at: 0 134 1509 1779 Size: 134 1375 270 Fragments arranged by size: 1375 270 134 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1456 1779 Size: 1456 323 BsrDI GCAATG_nn' Cuts at: 0 839 1779 Size: 839 940 BsrGI T'GTAC_A Cuts at: 0 375 1779 Size: 375 1404 BstAPI GCAn_nnn'nTGC Cuts at: 0 809 1779 Size: 809 970 BstEII G'GTnAC_C Cuts at: 0 737 1779 Size: 737 1042 BstXI CCAn_nnnn'nTGG Cuts at: 0 924 1779 Size: 924 855 Bsu36I CC'TnA_GG Cuts at: 0 319 1779 Size: 319 1460 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 182 1779 Size: 182 1597 BtsI GCAGTG_nn' Cuts at: 0 84 344 810 1320 1779 Size: 84 260 466 510 459 Fragments arranged by size: 510 466 459 260 84 DraI TTT'AAA Cuts at: 0 984 1779 Size: 984 795 DrdI GACnn_nn'nnGTC Cuts at: 0 1716 1779 Size: 1716 63 EaeI y'GGCC_r Cuts at: 0 221 1779 Size: 221 1558 EarI CTCTTCn'nnn_ Cuts at: 0 641 773 871 1779 Size: 641 132 98 908 Fragments arranged by size: 908 641 132 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1470 1779 Size: 1470 309 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 626 1779 Size: 626 1153 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 87 119 1357 1389 1779 Size: 87 32 1238 32 390 Fragments arranged by size: 1238 390 87 32 32 HincII GTy'rAC Cuts at: 0 468 1779 Size: 468 1311 HindIII A'AGCT_T Cuts at: 0 122 1779 Size: 122 1657 MfeI C'AATT_G Cuts at: 0 176 1779 Size: 176 1603 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1314 1623 1779 Size: 1314 309 156 Fragments arranged by size: 1314 309 156 MslI CAynn'nnrTG Cuts at: 0 178 1380 1779 Size: 178 1202 399 Fragments arranged by size: 1202 399 178 MspA1I CmG'CkG Cuts at: 0 15 397 1779 Size: 15 382 1382 Fragments arranged by size: 1382 382 15 NdeI CA'TA_TG Cuts at: 0 113 1779 Size: 113 1666 NsiI A_TGCA'T Cuts at: 0 503 507 1779 Size: 503 4 1272 Fragments arranged by size: 1272 503 4 NspI r_CATG'y Cuts at: 0 29 505 509 733 1593 1779 Size: 29 476 4 224 860 186 Fragments arranged by size: 860 476 224 186 29 4 PciI A'CATG_T Cuts at: 0 729 1589 1779 Size: 729 860 190 Fragments arranged by size: 860 729 190 PflMI CCAn_nnn'nTGG Cuts at: 0 397 1779 Size: 397 1382 PfoI T'CCnGG_A Cuts at: 0 1021 1779 Size: 1021 758 PsiI TTA'TAA Cuts at: 0 1688 1779 Size: 1688 91 PstI C_TGCA'G Cuts at: 0 351 1779 Size: 351 1428 PvuII CAG'CTG Cuts at: 0 397 1779 Size: 397 1382 SfcI C'TryA_G Cuts at: 0 228 347 1106 1352 1499 1779 Size: 228 119 759 246 147 280 Fragments arranged by size: 759 280 246 228 147 119 SmlI C'TyrA_G Cuts at: 0 618 1779 Size: 618 1161 SphI G_CATG'C Cuts at: 0 29 505 1779 Size: 29 476 1274 Fragments arranged by size: 1274 476 29 SspI AAT'ATT Cuts at: 0 760 1684 1779 Size: 760 924 95 Fragments arranged by size: 924 760 95 StyI C'CwwG_G Cuts at: 0 106 1779 Size: 106 1673 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 666 918 1315 1779 Size: 666 252 397 464 Fragments arranged by size: 666 464 397 252 TatI w'GTAC_w Cuts at: 0 375 564 1231 1779 Size: 375 189 667 548 Fragments arranged by size: 667 548 375 189 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1172 1204 1299 1331 1779 Size: 1172 32 95 32 448 Fragments arranged by size: 1172 448 95 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 924 1779 Size: 924 855 XmnI GAAnn'nnTTC Cuts at: 0 124 578 1779 Size: 124 454 1201 Fragments arranged by size: 1201 454 124 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AlwNI ApoI AseI AvaI BanI BbsI BbvCI BfrBI BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BstAPI BstEII BstXI Bsu36I BtgZI BtsI DraI DrdI EaeI EarI EciI Eco57MI Hin4I HincII HindIII MfeI MmeI MslI MspA1I NdeI NsiI NspI PciI PflMI PfoI PsiI PstI PvuII SfcI SmlI SphI SspI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmtI BpmI BsaAI BsaBI BsiEI BsiWI BsmBI BsrBI BsrFI BssHII BssSI BstBI BstYI BstZ17I BtgI ClaI CspCI DraIII EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XbaI XhoI XmaI ZraI