################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BfrI BglI BglII BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 588 CCGC CCGC(-3/-1) AvaI 594 CCCGGG C^[CT]CG[GA]G BbvI 406 GCTGC GCAGC(8/12) BcgI 606 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 369 TGATCA T^GATCA BlpI 232 GCTAAGC GC^T[ACGT]AGC Bpu1102I 232 GCTAAGC GC^T[ACGT]AGC BsmFI 47 GTCCC GGGAC(10/14) BsoBI 594 CCCGGG C^[CT]CG[GA]G BstXI 50 CCATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 135 GGATCT [GA]^GATC[CT] Cac8I 40 GCTAGC GC[ACGT]^[ACGT]GC CelII 232 GCTAAGC GC^T[ACGT]AGC DdeI 233 CTAAG C^T[ACGT]AG DsaI 448 CCGTGG C^C[GA][CT]GG EcoNI 413 CCTATCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 349 CCAAGG C^C[AT][AT]GG FbaI 369 TGATCA T^GATCA Fnu4HI 406 GCTGC GC^[ACGT]GC HapII 595 CCGG C^CGG HpaII 595 CCGG C^CGG ItaI 406 GCTGC GC^[ACGT]GC MflI 135 GGATCT [GA]^GATC[CT] MspI 595 CCGG C^CGG NheI 40 GCTAGC G^CTAGC NlaIV 622 GGCTCC GG[ACGT]^[ACGT]CC NspI 282 GCATGT [GA]CATG^[CT] PleI 529 GAGTC GAGTC(4/5) SmaI 594 CCCGGG CCC^GGG StyI 349 CCAAGG C^C[AT][AT]GG TfiI 607 GATTC G^A[AT]TC TseI 406 GCTGC G^C[AT]GC XcmI 497 CCAGGAAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 135 GGATCT [GA]^GATC[CT] XmaCI 594 CCCGGG C^CCGGG XmaI 594 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 4 GAATTT [GA]^AATT[CT] 190 AAATTT AlwI 135 GGATC GGATC(4/5) 598 GGATC ApoI 4 GAATTT [GA]^AATT[CT] 190 AAATTT BcnI 594 CCCGG CC^[GC]GG 595 CCGGG BfaI 41 CTAG C^TAG 334 CTAG BsgI 354 GTGCAG GTGCAG(16/14) 555 GTGCAG BsmAI 107 GTCTC GTCTC(1/5) 531 GTCTC Cfr13I 15 GGCCC G^G[ACGT]CC 392 GGCCC HaeIII 15 GGCC GG^CC 392 GGCC HinfI 529 GAGTC G^A[ACGT]TC 607 GATTC HphI 372 TCACC GGTGA(8/7) 542 TCACC MaeI 41 CTAG C^TAG 334 CTAG MspA1I 87 CAGCTG C[AC]G^C[GT]G 208 CAGCTG MwoI 158 GCCCCTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 167 GCCACTTTTGC NciI 594 CCCGG CC^[GC]GG 595 CCGGG NlaIII 142 CATG CATG^ 283 CATG PvuII 87 CAGCTG CAG^CTG 208 CAGCTG Sau96I 15 GGCCC G^G[ACGT]CC 392 GGCCC SfaNI 116 GCATC GCATC(5/9) 378 GCATC Tsp45I 79 GTGAC ^GT[GC]AC 541 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AluI BpmI BsaJI BsiYI BslI BsrI BssKI BstNI DpnII EarI EcoRII Ksp632I MaeIII MboI MboII MnlI MslI MvaI NdeII Sau3AI ScrFI Tsp509I TspRI