################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BglII BlnI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AflII 42 CTTAAG C^TTAAG AflIII 546 ACATGT A^C[GA][CT]GT AlwI 78 GGATC GGATC(4/5) Asp700I 274 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1034 GAGCAC G[AT]GC[AT]^C AvaII 396 GGACC G^G[AT]CC BalI 1685 TGGCCA TGG^CCA BanI 169 GGCACC G^G[CT][GA]CC BciVI 1196 GGATAC GTATCC(6/5) BfrI 42 CTTAAG C^TTAAG BglI 448 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 98 GCTTAGC GC^T[ACGT]AGC Bpu1102I 98 GCTTAGC GC^T[ACGT]AGC BsaAI 1231 TACGTG [CT]AC^GT[GA] BsiHKAI 1034 GAGCAC G[AT]GC[AT]^C BspDI 1552 ATCGAT AT^CGAT BspLU11I 546 ACATGT A^CATGT BstBI 1004 TTCGAA TT^CGAA BstEII 613 GGTTACC G^GT[ACGT]ACC BstPI 613 GGTTACC G^GT[ACGT]ACC BstYI 78 GGATCT [GA]^GATC[CT] CelII 98 GCTTAGC GC^T[ACGT]AGC ClaI 1552 ATCGAT AT^CGAT DraI 300 TTTAAA TTT^AAA DraII 1578 AGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 1685 TGGCCA [CT]^GGCC[GA] EcoO109I 1578 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 613 GGTTACC G^GT[ACGT]ACC HincII 257 GTCAAC GT[CT]^[GA]AC HindII 257 GTCAAC GT[CT]^[GA]AC MflI 78 GGATCT [GA]^GATC[CT] MluNI 1685 TGGCCA TGG^CCA MscI 1685 TGGCCA TGG^CCA MspA1I 429 CAGCTG C[AC]G^C[GT]G NspV 1004 TTCGAA TT^CGAA PflMI 202 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1611 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 215 CTGCAG CTGCA^G PvuII 429 CAGCTG CAG^CTG SfcI 215 CTGCAG C^T[GA][CT]AG SfuI 1004 TTCGAA TT^CGAA SphI 772 GCATGC GCATG^C StuI 1723 AGGCCT AGG^CCT Van91I 202 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 78 GGATCT [GA]^GATC[CT] XmnI 274 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 542 GTCTAC GT^[AC][GT]AC 1189 GTAGAC BpmI 432 CTGGAG CTGGAG(16/14) 1574 CTGGAG BsmI 995 GAATGC GAATGC(1/-1) 812 GCATTC BspMI 1059 GCAGGT ACCTGC(4/8) 1531 GCAGGT BstAPI 217 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 880 GCAGGCTTTGC Cfr13I 396 GGACC G^G[ACGT]CC 1579 GGCCC DpnII 79 GATC ^GATC 517 GATC DsaI 148 CCATGG C^C[GA][CT]GG 1054 CCATGG EcoT22I 159 ATGCAT ATGCA^T 1398 ATGCAT HindIII 4 AAGCTT A^AGCTT 240 AAGCTT MboI 79 GATC ^GATC 517 GATC NcoI 148 CCATGG C^CATGG 1054 CCATGG NdeI 417 CATATG CA^TATG 1538 CATATG NdeII 79 GATC ^GATC 517 GATC NsiI 159 ATGCAT ATGCA^T 1398 ATGCAT PleI 1570 GACTC GAGTC(4/5) 1439 GAGTC Sau3AI 79 GATC ^GATC 517 GATC Sau96I 396 GGACC G^G[ACGT]CC 1579 GGCCC TaqI 1005 TCGA T^CGA 1553 TCGA Tsp45I 58 GTCAC ^GT[GC]AC 1609 GTGAC TthHB8I 1005 TCGA T^CGA 1553 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AcsI AfaI AluI ApoI BbsI BbvI BmyI BpuAI BsaI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I DdeI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboII MnlI MseI MslI MvaI MwoI NlaIII NlaIV NspI RsaI ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp509I TspRI