(Circular) (Six-base) MAPSORT of: pFN21AE2007.seq Check: 6050 from: 1 to: 6454 pFN21AE2007 6454 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3055 3055 Size: 6454 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4264 278 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2548 3195 3797 1937 Size: 611 647 602 4594 Fragments arranged by size: 4594 647 611 602 Acc65I G'GTAC_C Cuts at: 3775 3775 Size: 6454 AclI AA'CG_TT Cuts at: 1563 4581 4954 1563 Size: 3018 373 3063 Fragments arranged by size: 3063 3018 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3440 4517 5901 6169 1632 Size: 1808 1077 1384 268 1917 Fragments arranged by size: 1917 1808 1384 1077 268 AflII C'TTAA_G Cuts at: 820 1017 2047 820 Size: 197 1030 5227 Fragments arranged by size: 5227 1030 197 AflIII A'CryG_T Cuts at: 1203 2551 5353 6330 1203 Size: 1348 2802 977 1327 Fragments arranged by size: 2802 1348 1327 977 AgeI A'CCGG_T Cuts at: 5285 5285 Size: 6454 AhdI GACnn_n'nnGTC Cuts at: 5183 5183 Size: 6454 AleI CACnn'nnGTG Cuts at: 1166 2222 3457 3686 1166 Size: 1056 1235 229 3934 Fragments arranged by size: 3934 1235 1056 229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6454 AlwNI CAG_nnn'CTG Cuts at: 4197 5769 4197 Size: 1572 4882 ApaLI G'TGCA_C Cuts at: 4511 5667 4511 Size: 1156 5298 ApoI r'AATT_y Cuts at: 1493 2533 2919 3105 3763 3989 4141 6250 1493 Size: 1040 386 186 658 226 152 2109 1697 Fragments arranged by size: 2109 1697 1040 658 386 226 186 152 AseI AT'TA_AT Cuts at: 160 1032 5008 160 Size: 872 3976 1606 Fragments arranged by size: 3976 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 AvaI C'yCGr_G Cuts at: 1870 1943 3779 1870 Size: 73 1836 4545 Fragments arranged by size: 4545 1836 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2180 2213 2180 Size: 33 6421 BamHI G'GATC_C Cuts at: 3784 4151 3784 Size: 367 6087 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 3775 5230 618 Size: 325 217 627 387 1601 1455 1842 Fragments arranged by size: 1842 1601 1455 627 387 325 217 BanII G_rGCy'C Cuts at: 1391 3773 1391 Size: 2382 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2134 2276 2956 928 Size: 971 235 142 680 4426 Fragments arranged by size: 4426 971 680 235 142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4645 4679 1903 Size: 34 2708 34 3678 Fragments arranged by size: 3678 2708 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3195 4346 5556 3195 Size: 1151 1210 4093 Fragments arranged by size: 4093 1210 1151 BclI T'GATC_A Cuts at: 1548 1548 Size: 6454 BfrBI ATG'CAT Cuts at: 2166 3405 2166 Size: 1239 5215 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2459 5065 136 Size: 107 122 71 631 605 787 2606 1525 Fragments arranged by size: 2606 1525 787 631 605 122 107 71 BglII A'GATC_T Cuts at: 6449 6449 Size: 6454 BlpI GC'TnA_GC Cuts at: 2104 3871 2104 Size: 1767 4687 Bme1580I G_kGCm'C Cuts at: 1790 2177 2207 4515 5671 1790 Size: 387 30 2308 1156 2573 Fragments arranged by size: 2573 2308 1156 387 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3056 4185 5143 Size: 416 53 83 399 24 1639 1129 958 Cuts at: 5143 442 Size: 1753 Fragments arranged by size: 1753 1639 1129 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6454 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2458 3600 5114 1239 Size: 1219 1142 1514 2579 Fragments arranged by size: 2579 1514 1219 1142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2061 2889 4579 5444 5742 2061 Size: 828 1690 865 298 2773 Fragments arranged by size: 2773 1690 865 828 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2567 2742 3045 3378 5117 882 Size: 617 1068 175 303 333 1739 2219 Fragments arranged by size: 2219 1739 1068 617 333 303 175 BsaAI yAC'GTr Cuts at: 493 1204 3238 4245 493 Size: 711 2034 1007 2702 Fragments arranged by size: 2702 2034 1007 711 BsaBI GATnn'nnATC Cuts at: 6448 6448 Size: 6454 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4261 4643 5278 275 Size: 53 83 186 936 2728 382 635 1451 Fragments arranged by size: 2728 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4887 5285 5559 5706 6117 4887 Size: 398 274 147 411 5224 Fragments arranged by size: 5224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2084 2114 1178 Size: 30 165 30 681 30 5518 Fragments arranged by size: 5518 681 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6454 BseYI C'CCAG_C Cuts at: 3417 5657 6153 3417 Size: 2240 496 3718 Fragments arranged by size: 3718 2240 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2904 3123 3555 1163 Size: 573 1168 219 432 4062 Fragments arranged by size: 4062 1168 573 432 219 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3904 4665 5693 1540 Size: 275 186 1903 761 1028 2301 Fragments arranged by size: 2301 1903 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3043 4515 4600 5671 3043 Size: 1472 85 1071 3826 Fragments arranged by size: 3826 1472 1071 85 BsmI GAATG_Cn' Cuts at: 2817 3006 3965 4058 2817 Size: 189 959 93 5213 Fragments arranged by size: 5213 959 189 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2207 3043 3773 4515 4600 5671 Size: 399 387 30 836 730 742 85 1071 Cuts at: 5671 1391 Size: 2174 Fragments arranged by size: 2174 1071 836 742 730 399 387 85 30 BspEI T'CCGG_A Cuts at: 6117 6117 Size: 6454 BspHI T'CATG_A Cuts at: 4343 6073 4343 Size: 1730 4724 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3055 3527 3809 844 Size: 2211 472 282 3489 Fragments arranged by size: 3489 2211 472 282 BsrBI CCG'CTC Cuts at: 1394 1823 4341 1394 Size: 429 2518 3507 Fragments arranged by size: 3507 2518 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2557 2797 2845 4949 5123 65 Size: 1183 1309 240 48 2104 174 1396 Fragments arranged by size: 2104 1396 1309 1183 240 174 48 BsrFI r'CCGG_y Cuts at: 1700 1810 5098 5285 1700 Size: 110 3288 187 2869 Fragments arranged by size: 3288 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6454 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6454 BssSI C'ACGA_G Cuts at: 4514 5526 4514 Size: 1012 5442 BstAPI GCAn_nnn'nTGC Cuts at: 794 2228 2891 794 Size: 1434 663 4357 Fragments arranged by size: 4357 1434 663 BstBI TT'CG_AA Cuts at: 3010 5258 3010 Size: 2248 4206 BstEII G'GTnAC_C Cuts at: 1184 2618 1184 Size: 1434 5020 BstYI r'GATC_y Cuts at: 1972 2083 3784 4151 4536 4553 6005 6091 6103 Size: 111 1701 367 385 17 1452 86 12 Cuts at: 6103 6449 1972 Size: 346 1977 Fragments arranged by size: 1977 1701 1452 385 367 346 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2153 3059 513 Size: 552 1088 906 3908 Fragments arranged by size: 3908 1088 906 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3277 487 Size: 683 56 2051 3664 Fragments arranged by size: 3664 2051 683 56 BtsI GCAGTG_nn' Cuts at: 2217 2229 3970 4764 4784 2217 Size: 12 1741 794 20 3887 Fragments arranged by size: 3887 1741 794 20 12 ClaI AT'CG_AT Cuts at: 1554 3558 1554 Size: 2004 4450 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2725 2760 529 Size: 35 2161 35 4223 Fragments arranged by size: 4223 2161 35 35 DraI TTT'AAA Cuts at: 2307 3758 2307 Size: 1451 5003 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5461 809 Size: 507 4145 1802 Fragments arranged by size: 4145 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3690 3901 4790 Size: 54 1425 50 275 18 1860 211 889 Cuts at: 4790 6419 8 Size: 1629 43 Fragments arranged by size: 1860 1629 1425 889 275 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3901 1537 Size: 275 2089 4090 Fragments arranged by size: 4090 2089 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4384 1353 Size: 3031 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5041 5415 5561 387 Size: 1064 138 3452 374 146 1280 Fragments arranged by size: 3452 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2458 3440 3600 4517 5114 5901 6169 Size: 393 826 982 160 917 597 787 268 Cuts at: 6169 1239 Size: 1524 Fragments arranged by size: 1524 982 917 826 787 597 393 268 160 EcoO109I rG'GnC_Cy Cuts at: 3584 3584 Size: 6454 EcoRI G'AATT_C Cuts at: 3763 4141 3763 Size: 378 6076 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2411 2411 Size: 6454 FspI TGC'GCA Cuts at: 4960 4960 Size: 6454 HaeII r_GCGC'y Cuts at: 1678 5601 1678 Size: 3923 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5095 5127 5169 5201 1373 Size: 32 135 32 3523 32 42 32 2626 Fragments arranged by size: 3523 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2264 3798 4044 669 Size: 988 281 326 1534 246 3079 Fragments arranged by size: 3079 1534 988 326 281 246 HindIII A'AGCT_T Cuts at: 748 2009 2245 748 Size: 1261 236 4957 Fragments arranged by size: 4957 1261 236 HpaI GTT'AAC Cuts at: 4044 4044 Size: 6454 KpnI G_GTAC'C Cuts at: 3779 3779 Size: 6454 MfeI C'AATT_G Cuts at: 4053 4053 Size: 6454 MluI A'CGCG_T Cuts at: 6330 6330 Size: 6454 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3104 3412 5568 5752 1305 Size: 1799 308 2156 184 2007 Fragments arranged by size: 2156 2007 1799 308 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3692 10 Size: 54 1425 343 1860 2772 Fragments arranged by size: 2772 1860 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 3457 3686 4412 4771 4930 518 Size: 648 1056 1235 229 726 359 159 2042 Fragments arranged by size: 2042 1235 1056 726 648 359 229 159 MspA1I CmG'CkG Cuts at: 1606 2436 3870 4547 5695 5940 1606 Size: 830 1434 677 1148 245 2120 Fragments arranged by size: 2120 1434 1148 830 677 245 NcoI C'CATG_G Cuts at: 513 1065 2153 3059 513 Size: 552 1088 906 3908 Fragments arranged by size: 3908 1088 906 552 NdeI CA'TA_TG Cuts at: 387 2423 3544 387 Size: 2036 1121 3297 Fragments arranged by size: 3297 2036 1121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6454 NotI GC'GGCC_GC Cuts at: 3901 3901 Size: 6454 NsiI A_TGCA'T Cuts at: 2168 3407 2168 Size: 1239 5215 NspI r_CATG'y Cuts at: 1132 1229 2322 2555 2781 3405 3812 5357 1132 Size: 97 1093 233 226 624 407 1545 2229 Fragments arranged by size: 2229 1545 1093 624 407 233 226 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6454 PciI A'CATG_T Cuts at: 2551 5353 2551 Size: 2802 3652 PflMI CCAn_nnn'nTGG Cuts at: 1108 2213 1108 Size: 1105 5349 PmeI GTTT'AAAC Cuts at: 3758 3758 Size: 6454 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4523 4555 6036 6068 4523 Size: 32 1481 32 4909 Fragments arranged by size: 4909 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3620 1613 Size: 2007 4447 PsiI TTA'TAA Cuts at: 4024 4024 Size: 6454 PstI C_TGCA'G Cuts at: 830 2224 3806 830 Size: 1394 1582 3478 Fragments arranged by size: 3478 1582 1394 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6454 PvuII CAG'CTG Cuts at: 2436 2436 Size: 6454 SalI G'TCGA_C Cuts at: 1936 3796 1936 Size: 1860 4594 SbfI CC_TGCA'GG Cuts at: 3806 3806 Size: 6454 ScaI AGT'ACT Cuts at: 4702 6414 4702 Size: 1712 4742 SfcI C'TryA_G Cuts at: 826 1045 2220 3802 4937 5618 5809 826 Size: 219 1175 1582 1135 681 191 1471 Fragments arranged by size: 1582 1471 1175 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6454 SmaI CCC'GGG Cuts at: 1872 3781 1872 Size: 1909 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2047 2076 2904 4558 5459 5721 Size: 197 926 104 29 828 1654 901 262 Cuts at: 5721 820 Size: 1553 Fragments arranged by size: 1654 1553 926 901 828 262 197 104 29 SnaBI TAC'GTA Cuts at: 493 4245 493 Size: 3752 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6454 SphI G_CATG'C Cuts at: 1132 2781 3812 1132 Size: 1649 1031 3774 Fragments arranged by size: 3774 1649 1031 SspI AAT'ATT Cuts at: 5 52 4378 5 Size: 47 4326 2081 Fragments arranged by size: 4326 2081 47 StuI AGG'CCT Cuts at: 1517 3730 1517 Size: 2213 4241 StyI C'CwwG_G Cuts at: 513 1065 2153 2473 2782 3059 3893 513 Size: 552 1088 320 309 277 834 3074 Fragments arranged by size: 3074 1088 834 552 320 309 277 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4481 4498 4651 4836 5281 325 Size: 1255 2901 17 153 185 445 1498 Fragments arranged by size: 2901 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2591 3744 4700 Size: 274 80 33 51 1443 614 1153 956 Cuts at: 4700 6412 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1153 956 614 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2084 2116 6414 6446 2084 Size: 32 4298 32 2092 Fragments arranged by size: 4298 2092 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6454 XbaI T'CTAG_A Cuts at: 3790 3790 Size: 6454 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6454 XmaI C'CCGG_G Cuts at: 1870 3779 1870 Size: 1909 4545 XmnI GAAnn'nnTTC Cuts at: 2283 4583 2283 Size: 2300 4154 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4262 276 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI