################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsmBI BsoBI Bsp1407I BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I CpoI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI Hin1I HindIII HpaI KasI KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NruI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 820 GGTACC G^GTACC Asp718I 820 GGTACC G^GTACC AspHI 1009 GAGCTC G[AT]GC[AT]^C BalI 1548 TGGCCA TGG^CCA BcnI 290 CCCGG CC^[GC]GG BfaI 542 CTAG C^TAG BglII 264 AGATCT A^GATCT BsaAI 701 TACGTG [CT]AC^GT[GA] BsaWI 1054 ACCGGA [AT]^CCGG[AT] BsiHKAI 1009 GAGCTC G[AT]GC[AT]^C BsiWI 699 CGTACG C^GTACG BsmAI 344 GTCTC GTCTC(1/5) BspDI 93 ATCGAT AT^CGAT BspHI 636 TCATGA T^CATGA BsrDI 552 GCAATG GCAATG(2/0) BstAPI 1072 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 738 CCAGGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 264 AGATCT [GA]^GATC[CT] CfoI 16 GCGC GCG^C ClaI 93 ATCGAT AT^CGAT DraI 828 TTTAAA TTT^AAA DraIII 1459 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 527 GAAGAG CTCTTC(1/4) EcoNI 1090 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1426 ATGCAT ATGCA^T HaeII 15 GGCGCT [GA]GCGC^[CT] HgaI 1601 GCGTC GACGC(5/10) HhaI 16 GCGC GCG^C HinP1I 16 GCGC G^CGC KpnI 820 GGTACC GGTAC^C Ksp632I 527 GAAGAG CTCTTC(1/4) MaeI 542 CTAG C^TAG MflI 264 AGATCT [GA]^GATC[CT] MluNI 1548 TGGCCA TGG^CCA MscI 1548 TGGCCA TGG^CCA MspA1I 1076 CAGCTG C[AC]G^C[GT]G NciI 290 CCCGG CC^[GC]GG NotI 49 GCGGCCGC GC^GGCCGC NsiI 1426 ATGCAT ATGCA^T PleI 1211 GAGTC GAGTC(4/5) PstI 43 CTGCAG CTGCA^G PvuII 1076 CAGCTG CAG^CTG RcaI 636 TCATGA T^CATGA SacI 1009 GAGCTC GAGCT^C SapI 527 GAAGAGC GCTCTTC(1/4) SbfI 42 CCTGCAGG CCTGCA^GG ScaI 874 AGTACT AGT^ACT SfaNI 188 GATGC GCATC(5/9) SmlI 381 CTCAAG C^T[CT][GA]AG Sse8387I 42 CCTGCAGG CCTGCA^GG SspI 1191 AATATT AAT^ATT StuI 925 AGGCCT AGG^CCT TfiI 858 GATTC G^A[AT]TC XcmI 1275 CCATGGACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 264 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 1240 GATCC GGATC(4/5) 1292 GGATC AspI 161 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1359 GACAGTGTC BanII 143 GGGCTC G[GA]GC[CT]^C 1009 GAGCTC BbsI 891 GAAGAC GAAGAC(2/6) 1606 GTCTTC BciVI 733 GGATAC GTATCC(6/5) 28 GTATCC BpuAI 891 GAAGAC GAAGAC(2/6) 1606 GTCTTC BseRI 1145 GAGGAG GAGGAG(10/8) 1208 GAGGAG BsiEI 50 CGGCCG CG[GA][CT]^CG 1416 CGGCCG BsmI 649 GAATGC GAATGC(1/-1) 1428 GCATTC EagI 50 CGGCCG C^GGCCG 1416 CGGCCG EclXI 50 CGGCCG C^GGCCG 1416 CGGCCG Eco52I 50 CGGCCG C^GGCCG 1416 CGGCCG HincII 559 GTCAAC GT[CT]^[GA]AC 1108 GTTGAC HindII 559 GTCAAC GT[CT]^[GA]AC 1108 GTTGAC HinfI 858 GATTC G^A[ACGT]TC 1211 GAGTC MaeII 698 ACGT A^CGT 702 ACGT MseI 434 TTAA T^TAA 829 TTAA PflFI 161 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1359 GACAGTGTC PflMI 460 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1339 CCAGCAGGTGG Tru9I 434 TTAA T^TAA 829 TTAA Tth111I 161 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1359 GACAGTGTC Van91I 460 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1339 CCAGCAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AfaI AluI AvaII BanI BbvI BmyI BpmI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I