Restriction map for
pFN21AE2460
(Circular) (Six-base) MAPSORT of: pFN21AE2460.seq Check: 881 from: 1 to: 6400
pFN21AE2460 6400 bp
With 182 enzymes: SgfI *
March 29, 2013 17:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3338 3338
Size: 6400
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4210 278
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3743 1937
Size: 1806 4594
Acc65I G'GTAC_C
Cuts at: 2825 3721 2825
Size: 896 5504
AclI AA'CG_TT
Cuts at: 1563 4527 4900 1563
Size: 2964 373 3063
Fragments arranged by size:
3063 2964 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 2778 3711 4463 5847 6115 1632
Size: 677 469 933 752 1384 268 1917
Fragments arranged by size:
1917 1384 933 752 677 469 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6203
AflIII A'CryG_T
Cuts at: 1203 3697 5299 6276 1203
Size: 2494 1602 977 1327
Fragments arranged by size:
2494 1602 1327 977
AgeI A'CCGG_T
Cuts at: 5231 5231
Size: 6400
AhdI GACnn_n'nnGTC
Cuts at: 5129 5129
Size: 6400
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6400
AlwNI CAG_nnn'CTG
Cuts at: 4143 5715 4143
Size: 1572 4828
ApaLI G'TGCA_C
Cuts at: 4457 5613 4457
Size: 1156 5244
ApoI r'AATT_y
Cuts at: 1493 3709 3935 4087 6196 1493
Size: 2216 226 152 2109 1697
Fragments arranged by size:
2216 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4954 160
Size: 872 3922 1606
Fragments arranged by size:
3922 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
AvaI C'yCGr_G
Cuts at: 1870 1943 3725 1870
Size: 73 1782 4545
Fragments arranged by size:
4545 1782 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2731 2764 2731
Size: 33 6367
BamHI G'GATC_C
Cuts at: 3730 4097 3730
Size: 367 6033
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2454 2825 3518 3721 5176
Size: 325 217 627 667 371 693 203 1455
Cuts at: 5176 618
Size: 1842
Fragments arranged by size:
1842 1455 693 667 627 371 325 217
203
BanII G_rGCy'C
Cuts at: 1391 2152 3018 3719 1391
Size: 761 866 701 4072
Fragments arranged by size:
4072 866 761 701
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2903 3604 928
Size: 971 1004 701 3724
Fragments arranged by size:
3724 1004 971 701
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4591 4625 1903
Size: 34 2654 34 3678
Fragments arranged by size:
3678 2654 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2044 2732 4292 5502 2044
Size: 688 1560 1210 2942
Fragments arranged by size:
2942 1560 1210 688
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6400
BfrBI ATG'CAT
Cuts at: 3433 3433
Size: 6400
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5011 136
Size: 107 122 71 631 605 3339 1525
Fragments arranged by size:
3339 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2269 6395 2269
Size: 4126 2274
BlpI GC'TnA_GC
Cuts at: 3817 3817
Size: 6400
Bme1580I G_kGCm'C
Cuts at: 1790 2395 2465 3523 4461 5617 1790
Size: 605 70 1058 938 1156 2573
Fragments arranged by size:
2573 1156 1058 938 605 70
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6400
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3334 3651 4131
Size: 416 53 83 399 24 1917 317 480
Cuts at: 4131 5089 442
Size: 958 1753
Fragments arranged by size:
1917 1753 958 480 416 399 317 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3081 3479 3583 5060 1239
Size: 1842 398 104 1477 2579
Fragments arranged by size:
2579 1842 1477 398 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2371 4525 5390 5688 2371
Size: 2154 865 298 3083
Fragments arranged by size:
3083 2154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5063 882
Size: 617 3564 2219
Fragments arranged by size:
3564 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2708 4191 493
Size: 711 1504 1483 2702
Fragments arranged by size:
2702 1504 1483 711
BsaBI GATnn'nnATC
Cuts at: 6394 6394
Size: 6400
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4207 4589 5224 275
Size: 53 83 186 936 2674 382 635 1451
Fragments arranged by size:
2674 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3059 4833 5231 5505 5652 6063 3059
Size: 1774 398 274 147 411 3396
Fragments arranged by size:
3396 1774 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2488 2518 1178
Size: 30 165 30 1085 30 5060
Fragments arranged by size:
5060 1085 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3165 3228 1186
Size: 1979 63 4358
Fragments arranged by size:
4358 1979 63
BseYI C'CCAG_C
Cuts at: 2139 2145 3343 5603 6099 2139
Size: 6 1198 2260 496 2440
Fragments arranged by size:
2440 2260 1198 496 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2462 2698 3243 3384 3464 1163
Size: 573 726 236 545 141 80 4099
Fragments arranged by size:
4099 726 573 545 236 141 80
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 3424 3850 4611 5639 1540
Size: 275 186 57 1366 426 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 426 275 186 57
BsiHKAI G_wGCw'C
Cuts at: 3018 4461 4546 5617 3018
Size: 1443 85 1071 3801
Fragments arranged by size:
3801 1443 1071 85
BsiWI C'GTAC_G
Cuts at: 2704 2704
Size: 6400
BsmI GAATG_Cn'
Cuts at: 2660 3433 3911 4004 2660
Size: 773 478 93 5056
Fragments arranged by size:
5056 773 478 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2395 2465 3018 3523 3719 4461
Size: 399 362 243 70 553 505 196 742
Cuts at: 4461 4546 5617 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 553 505 399 362 243
196 85 70
BspEI T'CCGG_A
Cuts at: 6063 6063
Size: 6400
BspHI T'CATG_A
Cuts at: 2641 4289 6019 2641
Size: 1648 1730 3022
Fragments arranged by size:
3022 1730 1648
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2055 2408 3338 3755 844
Size: 1211 353 930 417 3489
Fragments arranged by size:
3489 1211 930 417 353
BsrBI CCG'CTC
Cuts at: 1394 1823 4287 1394
Size: 429 2464 3507
Fragments arranged by size:
3507 2464 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2564 4895 5069 65
Size: 1183 1316 2331 174 1396
Fragments arranged by size:
2331 1396 1316 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5044 5231 1700
Size: 110 3234 187 2869
Fragments arranged by size:
3234 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6400
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6400
BssSI C'ACGA_G
Cuts at: 4460 5472 4460
Size: 1012 5388
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3083 794
Size: 2289 4111
BstBI TT'CG_AA
Cuts at: 5204 5204
Size: 6400
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6400
BstXI CCAn_nnnn'nTGG
Cuts at: 2750 2750
Size: 6400
BstYI r'GATC_y
Cuts at: 1972 2269 3730 4097 4482 4499 5951 6037 6049
Size: 297 1461 367 385 17 1452 86 12
Cuts at: 6049 6395 1972
Size: 346 1977
Fragments arranged by size:
1977 1461 1452 385 367 346 297 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2406 3167 3280 3523 3556 513
Size: 552 939 402 761 113 243 33 3357
Fragments arranged by size:
3357 939 761 552 402 243 113 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5661
Fragments arranged by size:
5661 683 56
BtsI GCAGTG_nn'
Cuts at: 3682 3916 4710 4730 3682
Size: 234 794 20 5352
Fragments arranged by size:
5352 794 234 20
ClaI AT'CG_AT
Cuts at: 1554 2099 1554
Size: 545 5855
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6365
DraI TTT'AAA
Cuts at: 2835 3704 2835
Size: 869 5531
DraIII CAC_nnn'GTG
Cuts at: 3469 3469
Size: 6400
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5407 809
Size: 507 4091 1802
Fragments arranged by size:
4091 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 3421 3553
Size: 54 1425 50 275 18 225 1366 132
Cuts at: 3553 3847 4736 6365 8
Size: 294 889 1629 43
Fragments arranged by size:
1629 1425 1366 889 294 275 225 132
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2055 3421 3847 1537
Size: 275 243 1366 426 4090
Fragments arranged by size:
4090 1366 426 275 243
EarI CTCTTCn'nnn_
Cuts at: 1353 2527 4330 1353
Size: 1174 1803 3423
Fragments arranged by size:
3423 1803 1174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4987 5361 5507 387
Size: 1064 138 3398 374 146 1280
Fragments arranged by size:
3398 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3016 3016
Size: 6400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2309 2778 3081 3479 3583 3711 4463
Size: 393 677 469 303 398 104 128 752
Cuts at: 4463 5060 5847 6115 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 752 677 597 469 398 393
303 268 128 104
EcoNI CCTnn'n_nnAGG
Cuts at: 3099 3099
Size: 6400
EcoO109I rG'GnC_Cy
Cuts at: 2499 2712 3340 3442 2499
Size: 213 628 102 5457
Fragments arranged by size:
5457 628 213 102
EcoRI G'AATT_C
Cuts at: 3709 4087 3709
Size: 378 6022
FspI TGC'GCA
Cuts at: 4906 4906
Size: 6400
HaeII r_GCGC'y
Cuts at: 1678 2024 5547 1678
Size: 346 3523 2531
Fragments arranged by size:
3523 2531 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2349 2381 2642 2674 3121
Size: 32 135 32 777 32 261 32 447
Cuts at: 3121 3153 5041 5073 5115 5147 1373
Size: 32 1888 32 42 32 2626
Fragments arranged by size:
2626 1888 777 447 261 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2566 3115 3744 3990 669
Size: 988 281 628 549 629 246 3079
Fragments arranged by size:
3079 988 629 628 549 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6400
HpaI GTT'AAC
Cuts at: 3990 3990
Size: 6400
KpnI G_GTAC'C
Cuts at: 2829 3725 2829
Size: 896 5504
MfeI C'AATT_G
Cuts at: 3999 3999
Size: 6400
MluI A'CGCG_T
Cuts at: 3697 6276 3697
Size: 2579 3821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2854 5514 5698 1305
Size: 1549 2660 184 2007
Fragments arranged by size:
2660 2007 1549 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3555 10
Size: 54 1425 343 1723 2855
Fragments arranged by size:
2855 1723 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4358 4717 4876 518
Size: 648 3192 359 159 2042
Fragments arranged by size:
3192 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3083 3816 4493 5641 5886 1606
Size: 1477 733 677 1148 245 2120
Fragments arranged by size:
2120 1477 1148 733 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2406 3280 3556 513
Size: 552 939 402 874 276 3357
Fragments arranged by size:
3357 939 874 552 402 276
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6400
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6400
NotI GC'GGCC_GC
Cuts at: 2055 3847 2055
Size: 1792 4608
NsiI A_TGCA'T
Cuts at: 3435 3435
Size: 6400
NspI r_CATG'y
Cuts at: 1132 1229 3758 5303 1132
Size: 97 2529 1545 2229
Fragments arranged by size:
2529 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3386 1348
Size: 2038 4362
PciI A'CATG_T
Cuts at: 5299 5299
Size: 6400
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2471 3350 1108
Size: 1363 879 4158
Fragments arranged by size:
4158 1363 879
PfoI T'CCnGG_A
Cuts at: 3097 3097
Size: 6400
PmeI GTTT'AAAC
Cuts at: 3704 3704
Size: 6400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4469 4501 5982 6014 4469
Size: 32 1481 32 4855
Fragments arranged by size:
4855 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2499 2712 3340 3442 2499
Size: 213 628 102 5457
Fragments arranged by size:
5457 628 213 102
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6400
PsiI TTA'TAA
Cuts at: 2700 3970 2700
Size: 1270 5130
PstI C_TGCA'G
Cuts at: 830 2052 3752 830
Size: 1222 1700 3478
Fragments arranged by size:
3478 1700 1222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6400
PvuII CAG'CTG
Cuts at: 3083 3083
Size: 6400
SacI G_AGCT'C
Cuts at: 3018 3018
Size: 6400
SalI G'TCGA_C
Cuts at: 1936 3742 1936
Size: 1806 4594
SanDI GG'GwC_CC
Cuts at: 3340 3340
Size: 6400
SapI GCTCTTCn'nnn_
Cuts at: 2527 2527
Size: 6400
SbfI CC_TGCA'GG
Cuts at: 2052 3752 2052
Size: 1700 4700
ScaI AGT'ACT
Cuts at: 2881 4648 6360 2881
Size: 1767 1712 2921
Fragments arranged by size:
2921 1767 1712
SfcI C'TryA_G
Cuts at: 826 1045 2048 2526 3072 3748 4883 5564 5755
Size: 219 1003 478 546 676 1135 681 191
Cuts at: 5755 826
Size: 1471
Fragments arranged by size:
1471 1135 1003 681 676 546 478 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6400
SmaI CCC'GGG
Cuts at: 1872 3727 1872
Size: 1855 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2386 4504 5405 5667 820
Size: 197 926 443 2118 901 262 1553
Fragments arranged by size:
2118 1553 926 901 443 262 197
SnaBI TAC'GTA
Cuts at: 493 4191 493
Size: 3698 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6400
SphI G_CATG'C
Cuts at: 1132 3758 1132
Size: 2626 3774
SspI AAT'ATT
Cuts at: 5 52 3198 4324 5
Size: 47 3146 1126 2081
Fragments arranged by size:
3146 2081 1126 47
StuI AGG'CCT
Cuts at: 1517 2932 1517
Size: 1415 4985
StyI C'CwwG_G
Cuts at: 513 1065 2004 2406 3280 3556 3839 513
Size: 552 939 402 874 276 283 3074
Fragments arranged by size:
3074 939 874 552 402 283 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4427 4444 4597 4782 5227 325
Size: 1255 2847 17 153 185 445 1498
Fragments arranged by size:
2847 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2879 3089 4646
Size: 274 80 33 51 1443 902 210 1557
Cuts at: 4646 6358 96
Size: 1712 138
Fragments arranged by size:
1712 1557 1443 902 274 210 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2488 2520 6360 6392 2488
Size: 32 3840 32 2496
Fragments arranged by size:
3840 2496 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2169 3367 1316
Size: 853 1198 4349
Fragments arranged by size:
4349 1198 853
XbaI T'CTAG_A
Cuts at: 3736 3736
Size: 6400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3287 3287
Size: 6400
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6400
XmaI C'CCGG_G
Cuts at: 1870 3725 1870
Size: 1855 4545
XmnI GAAnn'nnTTC
Cuts at: 4529 4529
Size: 6400
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4208 276
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI
SwaI