################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstPI BstUI CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII HphI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI StyI SwaI Tsp45I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 833 GTATAC GT^[AC][GT]AC AciI 1373 CCGC CCGC(-3/-1) AfaI 1327 GTAC GT^AC AflIII 432 ACATGT A^C[GA][CT]GT AlwNI 593 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 824 CCTCAGG CC^T[ACGT]AGG AseI 750 ATTAAT AT^TAAT AsnI 750 ATTAAT AT^TAAT Asp700I 733 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1451 GAGCTC G[AT]GC[AT]^C AspI 240 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 1451 GAGCTC G[GA]GC[CT]^C BclI 1433 TGATCA T^GATCA BmyI 1451 GAGCTC G[AGT]GC[ACT]^C BpmI 1025 CTGGAG CTGGAG(16/14) BsiHKAI 1451 GAGCTC G[AT]GC[AT]^C Bsp1286I 1451 GAGCTC G[AGT]GC[ACT]^C BspLU11I 432 ACATGT A^CATGT Bst1107I 833 GTATAC GTA^TAC BstBI 1232 TTCGAA TT^CGAA BstXI 1002 CCAACGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1021 GGATCT [GA]^GATC[CT] BstZ17I 833 GTATAC GTA^TAC Bsu36I 824 CCTCAGG CC^T[ACGT]AGG DrdI 1065 GACAAAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 824 CCTCAGG CC^T[ACGT]AGG EcoT22I 1 ATGCAT ATGCA^T FbaI 1433 TGATCA T^GATCA HaeIII 1475 GGCC GG^CC HincII 1527 GTCAAC GT[CT]^[GA]AC HindII 1527 GTCAAC GT[CT]^[GA]AC MaeIII 387 GTTAC ^GT[ACGT]AC MflI 1021 GGATCT [GA]^GATC[CT] MspA1I 593 CAGCTG C[AC]G^C[GT]G NdeI 1391 CATATG CA^TATG NheI 760 GCTAGC G^CTAGC NsiI 1 ATGCAT ATGCA^T NspV 1232 TTCGAA TT^CGAA PflFI 240 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1341 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 750 ATTAAT AT^TAAT PstI 1519 CTGCAG CTGCA^G PvuII 593 CAGCTG CAG^CTG RsaI 1327 GTAC GT^AC SacI 1451 GAGCTC GAGCT^C SexAI 241 ACCTGGT A^CC[AT]GGT SfaNI 1311 GATGC GCATC(5/9) SfuI 1232 TTCGAA TT^CGAA SmlI 807 CTTGAG C^T[CT][GA]AG SpeI 1041 ACTAGT A^CTAGT TspRI 789 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 240 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1353 TCTAGA T^CTAGA XcmI 1208 CCACTCTAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1021 GGATCT [GA]^GATC[CT] XmnI 733 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BbsI 928 GAAGAC GAAGAC(2/6) 456 GTCTTC BpuAI 928 GAAGAC GAAGAC(2/6) 456 GTCTTC BsaJI 270 CCCTGG C^C[ACGT][ACGT]GG 628 CCCTGG BseRI 62 CTCCTC GAGGAG(10/8) 182 GAGGAG BsgI 92 CTGCAC GTGCAG(16/14) 590 GTGCAG BsmI 737 GCATTC GAATGC(1/-1) 1466 GCATTC BsrI 390 ACTGG ACTGG(1/-1) 790 ACTGG Cac8I 760 GCTAGC GC[ACGT]^[ACGT]GC 882 GCTGGC DraII 1341 AGGTCCT [GA]G^G[ACGT]CC[CT] 1473 GGGGCCT EarI 374 CTCTTC CTCTTC(1/4) 951 GAAGAG EcoO109I 1341 AGGTCCT [GA]G^G[ACGT]CC[CT] 1473 GGGGCCT Ksp632I 374 CTCTTC CTCTTC(1/4) 951 GAAGAG MaeII 866 ACGT A^CGT 1005 ACGT NspI 432 ACATGT [GA]CATG^[CT] 802 ACATGC SspI 1186 AATATT AAT^ATT 1290 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AcsI AluI AlwI ApoI AvaII BbvI BfaI BsiYI BslI BsmAI BsmFI BsrDI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoNI EcoRII Fnu4HI FokI HinfI ItaI MaeI MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfcI TaqI TfiI Tru9I TseI Tsp509I TthHB8I