(Circular) (Six-base) MAPSORT of: pFN21AE6467.seq Check: 9090 from: 1 to: 6250 pFN21AE6467 6250 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4060 278 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2839 3593 1937 Size: 902 754 4594 Fragments arranged by size: 4594 902 754 Acc65I G'GTAC_C Cuts at: 3571 3571 Size: 6250 AclI AA'CG_TT Cuts at: 1563 4377 4750 1563 Size: 2814 373 3063 Fragments arranged by size: 3063 2814 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 4313 5697 5965 1632 Size: 654 2027 1384 268 1917 Fragments arranged by size: 2027 1917 1384 654 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6053 AflIII A'CryG_T Cuts at: 1203 2437 5149 6126 1203 Size: 1234 2712 977 1327 Fragments arranged by size: 2712 1327 1234 977 AgeI A'CCGG_T Cuts at: 5081 5081 Size: 6250 AhdI GACnn_n'nnGTC Cuts at: 4979 4979 Size: 6250 AleI CACnn'nnGTG Cuts at: 1166 3047 1166 Size: 1881 4369 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6250 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2898 2930 2898 Size: 32 6218 AlwNI CAG_nnn'CTG Cuts at: 2603 3993 5565 2603 Size: 1390 1572 3288 Fragments arranged by size: 3288 1572 1390 ApaLI G'TGCA_C Cuts at: 4307 5463 4307 Size: 1156 5094 ApoI r'AATT_y Cuts at: 1493 2284 2334 2627 3240 3559 3785 3937 6046 Size: 791 50 293 613 319 226 152 2109 Cuts at: 6046 1493 Size: 1697 Fragments arranged by size: 2109 1697 791 613 319 293 226 152 50 AseI AT'TA_AT Cuts at: 160 1032 2756 4804 160 Size: 872 1724 2048 1606 Fragments arranged by size: 2048 1724 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 AvaI C'yCGr_G Cuts at: 1870 1943 3575 1870 Size: 73 1632 4545 Fragments arranged by size: 4545 1632 73 BamHI G'GATC_C Cuts at: 3580 3947 3580 Size: 367 5883 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3571 5026 618 Size: 325 217 627 1784 1455 1842 Fragments arranged by size: 1842 1784 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3460 3569 1391 Size: 2069 109 4072 Fragments arranged by size: 4072 2069 109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2454 2940 928 Size: 971 555 486 4238 Fragments arranged by size: 4238 971 555 486 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4441 4475 1903 Size: 34 50 34 2420 34 3678 Fragments arranged by size: 3678 2420 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4142 5352 4142 Size: 1210 5040 BclI T'GATC_A Cuts at: 1548 3438 1548 Size: 1890 4360 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 6250 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4861 136 Size: 107 122 71 631 605 3189 1525 Fragments arranged by size: 3189 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6245 6245 Size: 6250 BlpI GC'TnA_GC Cuts at: 3667 3667 Size: 6250 Bme1580I G_kGCm'C Cuts at: 1790 4311 5467 1790 Size: 2521 1156 2573 Fragments arranged by size: 2573 2521 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6250 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3981 4939 442 Size: 416 53 83 399 24 2564 958 1753 Fragments arranged by size: 2564 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2769 1056 Size: 1713 4537 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3051 4910 1239 Size: 1812 1859 2579 Fragments arranged by size: 2579 1859 1812 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2833 4375 5240 5538 2833 Size: 1542 865 298 3545 Fragments arranged by size: 3545 1542 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4913 882 Size: 617 3414 2219 Fragments arranged by size: 3414 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4041 493 Size: 711 2837 2702 Fragments arranged by size: 2837 2702 711 BsaBI GATnn'nnATC Cuts at: 6244 6244 Size: 6250 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4057 4439 5074 275 Size: 53 83 186 936 2524 382 635 1451 Fragments arranged by size: 2524 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4683 5081 5355 5502 5913 4683 Size: 398 274 147 411 5020 Fragments arranged by size: 5020 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3150 3180 1178 Size: 30 165 30 1747 30 4248 Fragments arranged by size: 4248 1747 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2058 2202 1186 Size: 872 144 5234 Fragments arranged by size: 5234 872 144 BseYI C'CCAG_C Cuts at: 2507 5453 5949 2507 Size: 2946 496 2808 Fragments arranged by size: 2946 2808 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2082 2616 1163 Size: 573 346 534 4797 Fragments arranged by size: 4797 573 534 346 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3700 4461 5489 1540 Size: 275 186 1699 761 1028 2301 Fragments arranged by size: 2301 1699 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3460 4311 4396 5467 3460 Size: 851 85 1071 4243 Fragments arranged by size: 4243 1071 851 85 BsmI GAATG_Cn' Cuts at: 2742 3471 3761 3854 2742 Size: 729 290 93 5138 Fragments arranged by size: 5138 729 290 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3460 3569 4311 4396 5467 1391 Size: 399 1670 109 742 85 1071 2174 Fragments arranged by size: 2174 1670 1071 742 399 109 85 BspEI T'CCGG_A Cuts at: 5913 5913 Size: 6250 BspHI T'CATG_A Cuts at: 4139 5869 4139 Size: 1730 4520 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3605 844 Size: 2761 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4137 1394 Size: 429 2314 3507 Fragments arranged by size: 3507 2314 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2478 2645 3060 4745 4919 65 Size: 1183 1230 167 415 1685 174 1396 Fragments arranged by size: 1685 1396 1230 1183 415 174 167 BsrFI r'CCGG_y Cuts at: 1700 1810 4894 5081 1700 Size: 110 3084 187 2869 Fragments arranged by size: 3084 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6250 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6250 BssSI C'ACGA_G Cuts at: 4310 5322 4310 Size: 1012 5238 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6250 BstBI TT'CG_AA Cuts at: 3238 5054 3238 Size: 1816 4434 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6250 BstXI CCAn_nnnn'nTGG Cuts at: 3014 3014 Size: 6250 BstYI r'GATC_y Cuts at: 1972 3026 3580 3947 4332 4349 5801 5887 5899 Size: 1054 554 367 385 17 1452 86 12 Cuts at: 5899 6245 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1054 554 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2840 2840 Size: 6250 Bsu36I CC'TnA_GG Cuts at: 2830 2830 Size: 6250 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5698 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3162 487 Size: 683 56 1936 3575 Fragments arranged by size: 3575 1936 683 56 BtsI GCAGTG_nn' Cuts at: 3766 4560 4580 3766 Size: 794 20 5436 Fragments arranged by size: 5436 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6250 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2514 2549 529 Size: 35 1950 35 4230 Fragments arranged by size: 4230 1950 35 35 DraI TTT'AAA Cuts at: 3554 3554 Size: 6250 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3076 5257 809 Size: 507 1760 2181 1802 Fragments arranged by size: 2181 1802 1760 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3697 4586 6215 Size: 54 1425 50 275 18 1867 889 1629 Cuts at: 6215 8 Size: 43 Fragments arranged by size: 1867 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3697 1537 Size: 275 1885 4090 Fragments arranged by size: 4090 1885 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2385 2951 4180 1353 Size: 1032 566 1229 3423 Fragments arranged by size: 3423 1229 1032 566 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4837 5211 5357 387 Size: 1064 138 3248 374 146 1280 Fragments arranged by size: 3248 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3458 3458 Size: 6250 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 3051 4313 4910 5697 5965 1239 Size: 393 654 765 1262 597 787 268 1524 Fragments arranged by size: 1524 1262 787 765 654 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2184 2585 2863 2184 Size: 401 278 5571 Fragments arranged by size: 5571 401 278 EcoO109I rG'GnC_Cy Cuts at: 3347 3479 3347 Size: 132 6118 EcoRI G'AATT_C Cuts at: 3559 3937 3559 Size: 378 5872 FspI TGC'GCA Cuts at: 4756 4756 Size: 6250 HaeII r_GCGC'y Cuts at: 1678 5397 1678 Size: 3719 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3430 3462 4891 4923 4965 Size: 32 135 32 1858 32 1429 32 42 Cuts at: 4965 4997 1373 Size: 32 2626 Fragments arranged by size: 2626 1858 1429 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3534 3594 3840 669 Size: 988 281 1596 60 246 3079 Fragments arranged by size: 3079 1596 988 281 246 60 HindIII A'AGCT_T Cuts at: 748 748 Size: 6250 HpaI GTT'AAC Cuts at: 3840 3840 Size: 6250 KpnI G_GTAC'C Cuts at: 3575 3575 Size: 6250 MfeI C'AATT_G Cuts at: 3849 3849 Size: 6250 MluI A'CGCG_T Cuts at: 6126 6126 Size: 6250 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2771 3136 5364 5548 1305 Size: 1466 365 2228 184 2007 Fragments arranged by size: 2228 2007 1466 365 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4428 Fragments arranged by size: 4428 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2442 2705 3047 4208 4567 4726 518 Size: 648 1276 263 342 1161 359 159 2042 Fragments arranged by size: 2042 1276 1161 648 359 342 263 159 MspA1I CmG'CkG Cuts at: 1606 2600 3666 4343 5491 5736 1606 Size: 994 1066 677 1148 245 2120 Fragments arranged by size: 2120 1148 1066 994 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5698 NdeI CA'TA_TG Cuts at: 387 3397 387 Size: 3010 3240 NheI G'CTAG_C Cuts at: 1052 2765 1052 Size: 1713 4537 NotI GC'GGCC_GC Cuts at: 3697 3697 Size: 6250 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 6250 NspI r_CATG'y Cuts at: 1132 1229 2441 2811 3608 5153 1132 Size: 97 1212 370 797 1545 2229 Fragments arranged by size: 2229 1545 1212 797 370 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6250 PciI A'CATG_T Cuts at: 2437 5149 2437 Size: 2712 3538 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6250 PmeI GTTT'AAAC Cuts at: 3554 3554 Size: 6250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4319 4351 5832 5864 4319 Size: 32 1481 32 4705 Fragments arranged by size: 4705 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3347 3347 Size: 6250 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6250 PsiI TTA'TAA Cuts at: 3820 3820 Size: 6250 PstI C_TGCA'G Cuts at: 830 3528 3602 830 Size: 2698 74 3478 Fragments arranged by size: 3478 2698 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6250 PvuII CAG'CTG Cuts at: 2600 2600 Size: 6250 SacI G_AGCT'C Cuts at: 3460 3460 Size: 6250 SalI G'TCGA_C Cuts at: 1936 3592 1936 Size: 1656 4594 SbfI CC_TGCA'GG Cuts at: 3602 3602 Size: 6250 ScaI AGT'ACT Cuts at: 4498 6210 4498 Size: 1712 4538 SexAI A'CCwGG_T Cuts at: 2246 2246 Size: 6250 SfcI C'TryA_G Cuts at: 826 1045 2568 3327 3524 3598 4733 5414 5605 Size: 219 1523 759 197 74 1135 681 191 Cuts at: 5605 826 Size: 1471 Fragments arranged by size: 1523 1471 1135 759 681 219 197 191 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6250 SmaI CCC'GGG Cuts at: 1872 3577 1872 Size: 1705 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2812 4354 5255 5517 820 Size: 197 926 869 1542 901 262 1553 Fragments arranged by size: 1553 1542 926 901 869 262 197 SnaBI TAC'GTA Cuts at: 493 4041 493 Size: 3548 2702 SpeI A'CTAG_T Cuts at: 152 3046 152 Size: 2894 3356 SphI G_CATG'C Cuts at: 1132 3608 1132 Size: 2476 3774 SspI AAT'ATT Cuts at: 5 52 3193 3297 4174 5 Size: 47 3141 104 877 2081 Fragments arranged by size: 3141 2081 877 104 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6250 StyI C'CwwG_G Cuts at: 513 1065 3689 513 Size: 552 2624 3074 Fragments arranged by size: 3074 2624 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4277 4294 4447 4632 5077 325 Size: 1255 2697 17 153 185 445 1498 Fragments arranged by size: 2697 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3331 4496 6208 Size: 274 80 33 51 1443 1354 1165 1712 Cuts at: 6208 96 Size: 138 Fragments arranged by size: 1712 1443 1354 1165 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6210 6242 6210 Size: 32 6218 Tth111I GACn'n_nGTC Cuts at: 1316 2248 1316 Size: 932 5318 XbaI T'CTAG_A Cuts at: 3358 3586 3358 Size: 228 6022 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3220 3220 Size: 6250 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6250 XmaI C'CCGG_G Cuts at: 1870 3575 1870 Size: 1705 4545 XmnI GAAnn'nnTTC Cuts at: 2742 4379 2742 Size: 1637 4613 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4058 276 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI