################################## # NO CUT ENZYMES ( 225 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AcsI AcyI AfaI AflII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AclI 350 AACGTT AA^CGTT AflIII 217 ACATGT A^C[GA][CT]GT BbsI 366 GAAGAC GAAGAC(2/6) BpuAI 366 GAAGAC GAAGAC(2/6) BsaJI 111 CCAAGG C^C[ACGT][ACGT]GG BsiYI 107 CCAACCAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 107 CCAACCAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 115 GGGAC GGGAC(10/14) BspLU11I 217 ACATGT A^CATGT BsrDI 189 CATTGC GCAATG(2/0) BsrI 70 CCAGT ACTGG(1/-1) BstBI 338 TTCGAA TT^CGAA EcoT14I 111 CCAAGG C^C[AT][AT]GG HinfI 232 GATTC G^A[ACGT]TC MaeII 351 ACGT A^CGT MnlI 24 CCTC CCTC(7/6) NlaIII 218 CATG CATG^ NlaIV 64 GGAACC GG[ACGT]^[ACGT]CC NspI 217 ACATGT [GA]CATG^[CT] NspV 338 TTCGAA TT^CGAA Psp1406I 350 AACGTT AA^CGTT SfuI 338 TTCGAA TT^CGAA StyI 111 CCAAGG C^C[AT][AT]GG TaqI 339 TCGA T^CGA TfiI 232 GATTC G^A[AT]TC TspRI 197 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 339 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### MseI 146 TTAA T^TAA 257 TTAA MwoI 89 GCTGTTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 164 GCCCTTCCTGC SfaNI 32 GCATC GCATC(5/9) 98 GCATC Tru9I 146 TTAA T^TAA 257 TTAA Tsp509I 152 AATT ^AATT 180 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### MboII