Restriction map for
pFN21AE2422
(Circular) (Six-base) MAPSORT of: pFN21AE2422.seq Check: 5162 from: 1 to: 5353
pFN21AE2422 5353 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3163 278
Size: 53 83 186 936 1627 2468
Fragments arranged by size:
2468 1627 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2696 1937
Size: 759 4594
Acc65I G'GTAC_C
Cuts at: 2674 2674
Size: 5353
AclI AA'CG_TT
Cuts at: 1563 3480 3853 1563
Size: 1917 373 3063
Fragments arranged by size:
3063 1917 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2169 2507 3416 4800 5068 1632
Size: 537 338 909 1384 268 1917
Fragments arranged by size:
1917 1384 909 537 338 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5156
AflIII A'CryG_T
Cuts at: 1203 4252 5229 1203
Size: 3049 977 1327
Fragments arranged by size:
3049 1327 977
AgeI A'CCGG_T
Cuts at: 4184 4184
Size: 5353
AhdI GACnn_n'nnGTC
Cuts at: 4082 4082
Size: 5353
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5353
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5353
AlwNI CAG_nnn'CTG
Cuts at: 3096 4668 3096
Size: 1572 3781
ApaLI G'TGCA_C
Cuts at: 3410 4566 3410
Size: 1156 4197
ApoI r'AATT_y
Cuts at: 1493 2431 2579 2662 2888 3040 5149 1493
Size: 938 148 83 226 152 2109 1697
Fragments arranged by size:
2109 1697 938 226 152 148 83
AseI AT'TA_AT
Cuts at: 160 1032 3907 160
Size: 872 2875 1606
Fragments arranged by size:
2875 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5353
AvaI C'yCGr_G
Cuts at: 1870 1943 2678 1870
Size: 73 735 4545
Fragments arranged by size:
4545 735 73
BamHI G'GATC_C
Cuts at: 2683 3050 2683
Size: 367 4986
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2674 4129 618
Size: 325 217 627 887 1455 1842
Fragments arranged by size:
1842 1455 887 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 2672 1391
Size: 697 584 4072
Fragments arranged by size:
4072 697 584
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3544 3578 1903
Size: 34 1607 34 3678
Fragments arranged by size:
3678 1607 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3245 4455 3245
Size: 1210 4143
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5353
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3964 136
Size: 107 122 71 631 605 2292 1525
Fragments arranged by size:
2292 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2158 5348 2158
Size: 3190 2163
BlpI GC'TnA_GC
Cuts at: 2770 2770
Size: 5353
Bme1580I G_kGCm'C
Cuts at: 1790 3414 4570 1790
Size: 1624 1156 2573
Fragments arranged by size:
2573 1624 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5353
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3084 4042 442
Size: 416 53 83 399 24 1667 958 1753
Fragments arranged by size:
1753 1667 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4013 1239
Size: 2774 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3478 4343 4641 3478
Size: 865 298 4190
Fragments arranged by size:
4190 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4016 882
Size: 617 2517 2219
Fragments arranged by size:
2517 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3144 493
Size: 711 1940 2702
Fragments arranged by size:
2702 1940 711
BsaBI GATnn'nnATC
Cuts at: 5347 5347
Size: 5353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3160 3542 4177 275
Size: 53 83 186 936 1627 382 635 1451
Fragments arranged by size:
1627 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3786 4184 4458 4605 5016 3786
Size: 398 274 147 411 4123
Fragments arranged by size:
4123 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5128
Fragments arranged by size:
5128 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5353
BseYI C'CCAG_C
Cuts at: 4556 5052 4556
Size: 496 4857
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2319 1163
Size: 573 583 4197
Fragments arranged by size:
4197 583 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2803 3564 4592 1540
Size: 275 186 802 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 2333 3414 3499 4570 2088
Size: 245 1081 85 1071 2871
Fragments arranged by size:
2871 1081 1071 245 85
BsmI GAATG_Cn'
Cuts at: 2864 2957 2864
Size: 93 5260
BsmBI CGTCTCn'nnnn_
Cuts at: 2384 2384
Size: 5353
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2333 2672 3414 3499 4570 1391
Size: 399 298 245 339 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 339 298 245 85
BspEI T'CCGG_A
Cuts at: 5016 5016
Size: 5353
BspHI T'CATG_A
Cuts at: 3242 4972 3242
Size: 1730 3623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2708 844
Size: 1864 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3240 1394
Size: 429 1417 3507
Fragments arranged by size:
3507 1417 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3848 4022 65
Size: 1183 2600 174 1396
Fragments arranged by size:
2600 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3997 4184 1700
Size: 110 2187 187 2869
Fragments arranged by size:
2869 2187 187 110
BsrGI T'GTAC_A
Cuts at: 96 2240 96
Size: 2144 3209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5353
BssSI C'ACGA_G
Cuts at: 3413 4425 3413
Size: 1012 4341
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5353
BstBI TT'CG_AA
Cuts at: 4157 4157
Size: 5353
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5353
BstYI r'GATC_y
Cuts at: 1972 2158 2683 3050 3435 3452 4904 4990 5002
Size: 186 525 367 385 17 1452 86 12
Cuts at: 5002 5348 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 525 385 367 346 186 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4801
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4614
Fragments arranged by size:
4614 683 56
BtsI GCAGTG_nn'
Cuts at: 2122 2869 3663 3683 2122
Size: 747 794 20 3792
Fragments arranged by size:
3792 794 747 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5353
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5318
DraI TTT'AAA
Cuts at: 2044 2657 2044
Size: 613 4740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4360 809
Size: 507 3044 1802
Fragments arranged by size:
3044 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2800 3689 5318
Size: 54 1425 50 275 18 970 889 1629
Cuts at: 5318 8
Size: 43
Fragments arranged by size:
1629 1425 970 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2800 1537
Size: 275 988 4090
Fragments arranged by size:
4090 988 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2035 2241 2593 3283 1353
Size: 682 206 352 690 3423
Fragments arranged by size:
3423 690 682 352 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3940 4314 4460 387
Size: 1064 138 2351 374 146 1280
Fragments arranged by size:
2351 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2086 2086
Size: 5353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2169 2507 3416 4013 4800 5068 1239
Size: 393 537 338 909 597 787 268 1524
Fragments arranged by size:
1524 909 787 597 537 393 338 268
EcoRI G'AATT_C
Cuts at: 2579 2662 3040 2579
Size: 83 378 4892
Fragments arranged by size:
4892 378 83
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2126 2126
Size: 5353
FspI TGC'GCA
Cuts at: 3859 3859
Size: 5353
HaeII r_GCGC'y
Cuts at: 1678 4500 1678
Size: 2822 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3994 4026 4068 4100 1373
Size: 32 135 32 2422 32 42 32 2626
Fragments arranged by size:
2626 2422 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2182 2697 2943 669
Size: 988 281 244 515 246 3079
Fragments arranged by size:
3079 988 515 281 246 244
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5353
HpaI GTT'AAC
Cuts at: 2182 2943 2182
Size: 761 4592
KpnI G_GTAC'C
Cuts at: 2678 2678
Size: 5353
MfeI C'AATT_G
Cuts at: 2952 2952
Size: 5353
MluI A'CGCG_T
Cuts at: 5229 5229
Size: 5353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2361 4467 4651 1305
Size: 1056 2106 184 2007
Fragments arranged by size:
2106 2007 1056 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3531
Fragments arranged by size:
3531 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 2441 3311 3670 3829 518
Size: 648 913 362 870 359 159 2042
Fragments arranged by size:
2042 913 870 648 362 359 159
MspA1I CmG'CkG
Cuts at: 1606 2270 2769 3446 4594 4839 1606
Size: 664 499 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 499 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4801
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5353
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5353
NotI GC'GGCC_GC
Cuts at: 2800 2800
Size: 5353
NspI r_CATG'y
Cuts at: 1132 1229 2215 2711 4256 1132
Size: 97 986 496 1545 2229
Fragments arranged by size:
2229 1545 986 496 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5353
PciI A'CATG_T
Cuts at: 4252 4252
Size: 5353
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5353
PfoI T'CCnGG_A
Cuts at: 2574 2574
Size: 5353
PmeI GTTT'AAAC
Cuts at: 2657 2657
Size: 5353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 4935 4967 3422
Size: 32 1481 32 3808
Fragments arranged by size:
3808 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5353
PsiI TTA'TAA
Cuts at: 2923 2923
Size: 5353
PstI C_TGCA'G
Cuts at: 830 2705 830
Size: 1875 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5353
PvuII CAG'CTG
Cuts at: 2270 2270
Size: 5353
SacI G_AGCT'C
Cuts at: 2088 2088
Size: 5353
SalI G'TCGA_C
Cuts at: 1936 2695 1936
Size: 759 4594
SapI GCTCTTCn'nnn_
Cuts at: 2035 2035
Size: 5353
SbfI CC_TGCA'GG
Cuts at: 2705 2705
Size: 5353
ScaI AGT'ACT
Cuts at: 3601 5313 3601
Size: 1712 3641
SfcI C'TryA_G
Cuts at: 826 1045 2701 3836 4517 4708 826
Size: 219 1656 1135 681 191 1471
Fragments arranged by size:
1656 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5353
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5353
SmaI CCC'GGG
Cuts at: 1872 2680 1872
Size: 808 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3457 4358 4620 820
Size: 197 926 1514 901 262 1553
Fragments arranged by size:
1553 1514 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3144 493
Size: 2651 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5353
SphI G_CATG'C
Cuts at: 1132 2711 1132
Size: 1579 3774
SspI AAT'ATT
Cuts at: 5 52 3277 5
Size: 47 3225 2081
Fragments arranged by size:
3225 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5353
StyI C'CwwG_G
Cuts at: 513 1065 2792 513
Size: 552 1727 3074
Fragments arranged by size:
3074 1727 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3380 3397 3550 3735 4180 325
Size: 1255 1800 17 153 185 445 1498
Fragments arranged by size:
1800 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2240 3599 5311
Size: 274 80 33 51 1443 263 1359 1712
Cuts at: 5311 96
Size: 138
Fragments arranged by size:
1712 1443 1359 274 263 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5313 5345 5313
Size: 32 5321
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5353
XbaI T'CTAG_A
Cuts at: 2689 2689
Size: 5353
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5353
XmaI C'CCGG_G
Cuts at: 1870 2678 1870
Size: 808 4545
XmnI GAAnn'nnTTC
Cuts at: 3482 3482
Size: 5353
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3161 276
Size: 53 83 186 936 1627 2468
Fragments arranged by size:
2468 1627 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI