################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII AccI AccII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AvaI AviII AvrII BalI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I FbaI FseI FspI HaeII HhaI HinP1I HpaI KasI KspI MaeII MamI MfeI MluI MluNI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NotI NruI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 1157 GGTACC G^GTACC AccIII 779 TCCGGA T^CCGGA AcsI 295 GAATTT [GA]^AATT[CT] AcyI 1048 GACGCC G[GA]^CG[CT]C ApoI 295 GAATTT [GA]^AATT[CT] AseI 664 ATTAAT AT^TAAT AsnI 664 ATTAAT AT^TAAT Asp718I 1157 GGTACC G^GTACC AspHI 950 GAGCAC G[AT]GC[AT]^C BamHI 387 GGATCC G^GATCC BanI 1157 GGTACC G^G[CT][GA]CC BbsI 144 GAAGAC GAAGAC(2/6) BmyI 950 GAGCAC G[AGT]GC[ACT]^C BpmI 1118 CTGGAG CTGGAG(16/14) BpuAI 144 GAAGAC GAAGAC(2/6) BsaHI 1048 GACGCC G[GA]^CG[CT]C BsaI 775 GGTCTC GGTCTC(1/5) BsaWI 779 TCCGGA [AT]^CCGG[AT] BseAI 779 TCCGGA T^CCGGA BsiHKAI 950 GAGCAC G[AT]GC[AT]^C BsiYI 624 CCTGCAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 624 CCTGCAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 950 GAGCAC G[AGT]GC[ACT]^C BspEI 779 TCCGGA T^CCGGA BstBI 759 TTCGAA TT^CGAA DraII 450 AGGACCC [GA]G^G[ACGT]CC[CT] EaeI 10 CGGCCA [CT]^GGCC[GA] EarI 595 GAAGAG CTCTTC(1/4) EcoO109I 450 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 1150 GATATC GAT^ATC FauI 1239 GCGGG CCCGC(4/6) HapII 780 CCGG C^CGG Hin1I 1048 GACGCC G[GA]^CG[CT]C HincII 326 GTCAAC GT[CT]^[GA]AC HindII 326 GTCAAC GT[CT]^[GA]AC HindIII 1037 AAGCTT A^AGCTT HpaII 780 CCGG C^CGG KpnI 1157 GGTACC GGTAC^C Ksp632I 595 GAAGAG CTCTTC(1/4) MroI 779 TCCGGA T^CCGGA MspI 780 CCGG C^CGG NdeI 352 CATATG CA^TATG NheI 619 GCTAGC G^CTAGC NspI 1102 GCATGC [GA]CATG^[CT] NspV 759 TTCGAA TT^CGAA PacI 661 TTAATTAA TTAAT^TAA PpuMI 450 AGGACCC [GA]G^G[AT]CC[CT] PshBI 664 ATTAAT AT^TAAT PstI 625 CTGCAG CTGCA^G SexAI 918 ACCAGGT A^CC[AT]GGT SfuI 759 TTCGAA TT^CGAA SmlI 1034 CTCAAG C^T[CT][GA]AG SphI 1102 GCATGC GCATG^C TfiI 250 GAATC G^A[AT]TC XcmI 1107 CCAGGGTGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 533 GCGG CCGC(-3/-1) 1239 GCGG AfaI 877 GTAC GT^AC 1158 GTAC AvaII 451 GGACC G^G[AT]CC 1123 GGACC BsmFI 583 GGGAC GGGAC(10/14) 1241 GGGAC BsrDI 526 CATTGC GCAATG(2/0) 1025 GCAATG BstF5I 902 CATCC GGATG(2/0) 633 GGATG BstYI 387 GGATCC [GA]^GATC[CT] 465 AGATCC FokI 902 CATCC GGATG(9/13) 633 GGATG HgaI 1012 GACGC GACGC(5/10) 1048 GACGC MflI 387 GGATCC [GA]^GATC[CT] 465 AGATCC MslI 643 CATCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 820 CATGACTATG RsaI 877 GTAC GT^AC 1158 GTAC TaqI 760 TCGA T^CGA 987 TCGA TthHB8I 760 TCGA T^CGA 987 TCGA XhoII 387 GGATCC [GA]^GATC[CT] 465 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AluI AlwI BbvI BfaI BsaJI BsmAI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT22I Fnu4HI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV NsiI PleI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI