Restriction map for
pFN21AE6371
(Circular) (Six-base) MAPSORT of: pFN21AE6371.seq Check: 4702 from: 1 to: 5974
pFN21AE6371 5974 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3162 3295 3162
Size: 133 5841
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4037 5421 5689 1632
Size: 2405 1384 268 1917
Fragments arranged by size:
2405 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2106 2138 2106
Size: 32 5942
AlwNI CAG_nnn'CTG
Cuts at: 3717 5289 3717
Size: 1572 4402
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2300 3283 3509 3661 5770 1493
Size: 807 983 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 807 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2670 4528 160
Size: 872 1638 1858 1606
Fragments arranged by size:
1858 1638 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 3299 1870
Size: 73 1356 4545
Fragments arranged by size:
4545 1356 73
BamHI G'GATC_C
Cuts at: 2392 3304 3671 2392
Size: 912 367 4695
Fragments arranged by size:
4695 912 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3162 3295 4750 618
Size: 325 217 627 1375 133 1455 1842
Fragments arranged by size:
1842 1455 1375 627 325 217 133
BanII G_rGCy'C
Cuts at: 1391 3293 1391
Size: 1902 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2156 928
Size: 971 257 4746
Fragments arranged by size:
4746 971 257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BfrBI ATG'CAT
Cuts at: 2273 2906 3107 2273
Size: 633 201 5140
Fragments arranged by size:
5140 633 201
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 4035 5191 1790
Size: 2245 1156 2573
Fragments arranged by size:
2573 2245 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3705 4663 442
Size: 416 53 83 399 24 2288 958 1753
Fragments arranged by size:
2288 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2628 1056
Size: 1572 4402
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2712 2912 2712
Size: 200 5774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3144 4634 1239
Size: 1905 1490 2579
Fragments arranged by size:
2579 1905 1490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3024 4099 4964 5262 3024
Size: 1075 865 298 3736
Fragments arranged by size:
3736 1075 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2786 4637 882
Size: 617 1287 1851 2219
Fragments arranged by size:
2219 1851 1287 617
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3054 3781 4163 4798
Size: 53 83 186 936 1521 727 382 635
Cuts at: 4798 275
Size: 1451
Fragments arranged by size:
1521 1451 936 727 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2784 4407 4805 5079 5226 5637 2784
Size: 1623 398 274 147 411 3121
Fragments arranged by size:
3121 1623 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2766 2796 1178
Size: 30 165 30 1363 30 4356
Fragments arranged by size:
4356 1363 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5974
BseYI C'CCAG_C
Cuts at: 3183 5177 5673 3183
Size: 1994 496 3484
Fragments arranged by size:
3484 1994 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2959 4035 4120 5191 2959
Size: 1076 85 1071 3742
Fragments arranged by size:
3742 1076 1071 85
BsmI GAATG_Cn'
Cuts at: 3485 3578 3485
Size: 93 5881
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2959 3293 4035 4120 5191 1391
Size: 399 1169 334 742 85 1071 2174
Fragments arranged by size:
2174 1169 1071 742 399 334 85
BspEI T'CCGG_A
Cuts at: 2784 5637 2784
Size: 2853 3121
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2530 3037 4469 4643 65
Size: 1183 1282 507 1432 174 1396
Fragments arranged by size:
1432 1396 1282 1183 507 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 2765 4778 2765
Size: 2013 3961
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2392 2470 3304 3671 4056 4073 5525 5611
Size: 420 78 834 367 385 17 1452 86
Cuts at: 5611 5623 5969 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 834 420 385 367 346 86
78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5422
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2633 487
Size: 683 56 1407 3828
Fragments arranged by size:
3828 1407 683 56
BtsI GCAGTG_nn'
Cuts at: 2109 2639 3490 4284 4304 2109
Size: 530 851 794 20 3779
Fragments arranged by size:
3779 851 794 530 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2665 2700 529
Size: 35 2101 35 3803
Fragments arranged by size:
3803 2101 35 35
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 3421 4310
Size: 54 1425 50 275 18 185 1406 889
Cuts at: 4310 5939 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1406 889 275 185 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2595 3904 1353
Size: 1242 1309 3423
Fragments arranged by size:
3423 1309 1242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3144 4037 4634 5421 5689 1239
Size: 393 1512 893 597 787 268 1524
Fragments arranged by size:
1524 1512 893 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2456 2456
Size: 5974
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
EcoRV GAT'ATC
Cuts at: 3157 3157
Size: 5974
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 5121 1678
Size: 3443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2125 2157 2462 2494 4615
Size: 32 135 32 553 32 305 32 2121
Cuts at: 4615 4647 4689 4721 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2121 553 305 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2333 3318 3564 669
Size: 988 281 395 985 246 3079
Fragments arranged by size:
3079 988 985 395 281 246
HindIII A'AGCT_T
Cuts at: 748 3042 748
Size: 2294 3680
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3166 3299 3166
Size: 133 5841
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2652 2829 3932 4291 4450 518
Size: 648 1486 177 1103 359 159 2042
Fragments arranged by size:
2042 1486 1103 648 359 177 159
MspA1I CmG'CkG
Cuts at: 1606 3390 4067 5215 5460 1606
Size: 1784 677 1148 245 2120
Fragments arranged by size:
2120 1784 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5422
NdeI CA'TA_TG
Cuts at: 387 2358 387
Size: 1971 4003
NheI G'CTAG_C
Cuts at: 1052 2624 1052
Size: 1572 4402
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NsiI A_TGCA'T
Cuts at: 2275 2908 3109 2275
Size: 633 201 5140
Fragments arranged by size:
5140 633 201
NspI r_CATG'y
Cuts at: 1132 1229 3111 3332 4877 1132
Size: 97 1882 221 1545 2229
Fragments arranged by size:
2229 1882 1545 221 97
PacI TTA_AT'TAA
Cuts at: 2670 2670
Size: 5974
PasI CC'CwG_GG
Cuts at: 1348 2058 1348
Size: 710 5264
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2456 2456
Size: 5974
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 2280 3544 2280
Size: 1264 4710
PstI C_TGCA'G
Cuts at: 830 2634 3326 830
Size: 1804 692 3478
Fragments arranged by size:
3478 1804 692
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SexAI A'CCwGG_T
Cuts at: 2923 2923
Size: 5974
SfcI C'TryA_G
Cuts at: 826 1045 2164 2403 2451 2630 3322 4457 5138
Size: 219 1119 239 48 179 692 1135 681
Cuts at: 5138 5329 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1119 692 681 239 219 191
179 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3039 4078 4979 5241 820
Size: 197 926 1096 1039 901 262 1553
Fragments arranged by size:
1553 1096 1039 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3111 3332 1132
Size: 1979 221 3774
Fragments arranged by size:
3774 1979 221
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 3413 513
Size: 552 2348 3074
Fragments arranged by size:
3074 2348 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2832 4001 4018 4171 4356 4801 325
Size: 1255 1252 1169 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1252 1169 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2881 4220 5932
Size: 274 80 33 51 1443 904 1339 1712
Cuts at: 5932 96
Size: 138
Fragments arranged by size:
1712 1443 1339 904 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2569 2601 5934 5966 2569
Size: 32 3333 32 2577
Fragments arranged by size:
3333 2577 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3119 3119
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI