################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccIII AclI AgeI AocI ApaI AscI AseI AsnI Asp700I AspI AviII AvrII BcgI BglII BlnI BpmI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssSI Bst1107I BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco52I Eco81I FseI FspI HpaI MfeI MluI MroI MunI NdeI NotI NruI PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SfiI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 3907 GGTACC G^GTACC AccI 1644 GTCTAC GT^[AC][GT]AC AflII 2854 CTTAAG C^TTAAG AflIII 1494 ACGTGT A^C[GA][CT]GT Alw44I 3496 GTGCAC G^TGCAC Aor51HI 66 AGCGCT AGC^GCT ApaLI 3496 GTGCAC G^TGCAC Asp718I 3907 GGTACC G^GTACC BalI 3854 TGGCCA TGG^CCA BbrPI 3460 CACGTG CAC^GTG BbvCI 3406 CCTCAGC CCTCAGC(-5/-2) BclI 807 TGATCA T^GATCA BfrI 2854 CTTAAG C^TTAAG BlpI 1758 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1758 GCTGAGC GC^T[ACGT]AGC BsaAI 3460 CACGTG [CT]AC^GT[GA] BsaBI 2361 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 3173 TCATGA T^CATGA BspMI 213 GCAGGT ACCTGC(4/8) BsrBI 52 CCGCTC CCGCTC(-3/-3) BssHII 29 GCGCGC G^CGCGC BstAPI 213 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3485 TTCGAA TT^CGAA CelII 1758 GCTGAGC GC^T[ACGT]AGC EarI 3662 GAAGAG CTCTTC(1/4) Eco47III 66 AGCGCT AGC^GCT EcoNI 1529 CCTGCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 547 GAATTC G^AATTC EcoRV 276 GATATC GAT^ATC EcoT22I 592 ATGCAT ATGCA^T FbaI 807 TGATCA T^GATCA KpnI 3907 GGTACC GGTAC^C Ksp632I 3662 GAAGAG CTCTTC(1/4) KspI 107 CCGCGG CCGC^GG MamI 2361 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3854 TGGCCA TGG^CCA MscI 3854 TGGCCA TGG^CCA NcoI 1140 CCATGG C^CATGG NheI 1575 GCTAGC G^CTAGC NsiI 592 ATGCAT ATGCA^T NspV 3485 TTCGAA TT^CGAA PaeR7I 3651 CTCGAG C^TCGAG PmaCI 3460 CACGTG CAC^GTG PmlI 3460 CACGTG CAC^GTG RcaI 3173 TCATGA T^CATGA SacII 107 CCGCGG CCGC^GG SapI 3662 GAAGAGC GCTCTTC(1/4) SexAI 634 ACCAGGT A^CC[AT]GGT SfuI 3485 TTCGAA TT^CGAA SgrAI 16 CGCCGGCG C[GA]^CCGG[CT]G SpeI 3778 ACTAGT A^CTAGT XhoI 3651 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AhdI 2064 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3548 GACTCACAGTC AspEI 2064 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3548 GACTCACAGTC AvaI 146 CCCGAG C^[CT]CG[GA]G 3651 CTCGAG BamHI 1731 GGATCC G^GATCC 2586 GGATCC BbsI 1550 GTCTTC GAAGAC(2/6) 3285 GTCTTC BciVI 630 GGATAC GTATCC(6/5) 2648 GTATCC BglI 17 GCCGGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3626 GCCGGCTGGGC BpuAI 1550 GTCTTC GAAGAC(2/6) 3285 GTCTTC BsoBI 146 CCCGAG C^[CT]CG[GA]G 3651 CTCGAG BstEII 249 GGTCACC G^GT[ACGT]ACC 1995 GGTTACC BstPI 249 GGTCACC G^GT[ACGT]ACC 1995 GGTTACC DraII 215 AGGTCCT [GA]G^G[ACGT]CC[CT] 3894 GGGACCC Eam1105I 2064 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3548 GACTCACAGTC EcoO109I 215 AGGTCCT [GA]G^G[ACGT]CC[CT] 3894 GGGACCC EcoO65I 249 GGTCACC G^GT[ACGT]ACC 1995 GGTTACC PpuMI 215 AGGTCCT [GA]G^G[AT]CC[CT] 3894 GGGACCC PvuII 1756 CAGCTG CAG^CTG 3806 CAGCTG SacI 1586 GAGCTC GAGCT^C 3195 GAGCTC ScaI 1040 AGTACT AGT^ACT 2517 AGTACT SspI 937 AATATT AAT^ATT 1615 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII