(Linear) (Six-base) MAPSORT of: KIEE9457.seq Check: 5239 from: 1 to: 3963 KIEE9457.orf With 182 enzymes: SgfI * December 27, 2011 16:40 .. AccI GT'mk_AC Cuts at: 0 1645 3963 Size: 1645 2318 Acc65I G'GTAC_C Cuts at: 0 3907 3963 Size: 3907 56 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 1065 1537 3096 3963 Size: 307 758 472 1559 867 Fragments arranged by size: 1559 867 758 472 307 AfeI AGC'GCT Cuts at: 0 68 3963 Size: 68 3895 AflII C'TTAA_G Cuts at: 0 2854 3963 Size: 2854 1109 AflIII A'CryG_T Cuts at: 0 1494 3963 Size: 1494 2469 AhdI GACnn_n'nnGTC Cuts at: 0 2069 3553 3963 Size: 2069 1484 410 Fragments arranged by size: 2069 1484 410 AleI CACnn'nnGTG Cuts at: 0 679 3137 3963 Size: 679 2458 826 Fragments arranged by size: 2458 826 679 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2739 3279 3377 3387 3963 Size: 2739 540 98 10 576 Fragments arranged by size: 2739 576 540 98 10 AlwNI CAG_nnn'CTG Cuts at: 0 219 693 1332 1696 1948 2095 2471 3963 Size: 219 474 639 364 252 147 376 1492 Fragments arranged by size: 1492 639 474 376 364 252 219 147 ApaLI G'TGCA_C Cuts at: 0 3496 3963 Size: 3496 467 ApoI r'AATT_y Cuts at: 0 486 547 1405 1953 1962 3321 3753 3963 Size: 486 61 858 548 9 1359 432 210 Fragments arranged by size: 1359 858 548 486 432 210 61 9 AvaI C'yCGr_G Cuts at: 0 146 3651 3963 Size: 146 3505 312 Fragments arranged by size: 3505 312 146 BamHI G'GATC_C Cuts at: 0 1731 2586 3963 Size: 1731 855 1377 Fragments arranged by size: 1731 1377 855 BanI G'GyrC_C Cuts at: 0 103 169 367 2156 3255 3465 3907 3963 Size: 103 66 198 1789 1099 210 442 56 Fragments arranged by size: 1789 1099 442 210 198 103 66 56 BanII G_rGCy'C Cuts at: 0 1590 2864 3199 3963 Size: 1590 1274 335 764 Fragments arranged by size: 1590 1274 764 335 BbeI G_GCGC'C Cuts at: 0 107 173 3469 3963 Size: 107 66 3296 494 Fragments arranged by size: 3296 494 107 66 BbsI GAAGACnn'nnnn_ Cuts at: 0 1543 3278 3963 Size: 1543 1735 685 Fragments arranged by size: 1735 1543 685 BbvCI CC'TCA_GC Cuts at: 0 3407 3963 Size: 3407 556 BciVI GTATCCnnnnn_n' Cuts at: 0 624 2659 3963 Size: 624 2035 1304 Fragments arranged by size: 2035 1304 624 BclI T'GATC_A Cuts at: 0 807 3963 Size: 807 3156 BfrBI ATG'CAT Cuts at: 0 594 3963 Size: 594 3369 BglI GCCn_nnn'nGGC Cuts at: 0 23 3632 3963 Size: 23 3609 331 Fragments arranged by size: 3609 331 23 BlpI GC'TnA_GC Cuts at: 0 1759 3963 Size: 1759 2204 Bme1580I G_kGCm'C Cuts at: 0 924 3500 3963 Size: 924 2576 463 Fragments arranged by size: 2576 924 463 BmgBI CAC'GTC Cuts at: 0 1452 3963 Size: 1452 2511 BmtI G_CTAG'C Cuts at: 0 1579 3963 Size: 1579 2384 Bpu10I CC'TnA_GC Cuts at: 0 2837 3407 3963 Size: 2837 570 556 Fragments arranged by size: 2837 570 556 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 491 2359 3486 3963 Size: 491 1868 1127 477 Fragments arranged by size: 1868 1127 491 477 BsaAI yAC'GTr Cuts at: 0 3462 3963 Size: 3462 501 BsaBI GATnn'nnATC Cuts at: 0 2365 3963 Size: 2365 1598 BsaHI Gr'CG_yC Cuts at: 0 15 104 170 3466 3963 Size: 15 89 66 3296 497 Fragments arranged by size: 3296 497 89 66 15 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 889 919 1577 1607 3963 Size: 889 30 658 30 2356 Fragments arranged by size: 2356 889 658 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2299 2884 3669 3963 Size: 2299 585 785 294 Fragments arranged by size: 2299 785 585 294 BseYI C'CCAG_C Cuts at: 0 81 599 3630 3704 3963 Size: 81 518 3031 74 259 Fragments arranged by size: 3031 518 259 81 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 213 1019 1564 3625 3963 Size: 213 806 545 2061 338 Fragments arranged by size: 2061 806 545 338 213 BsiHKAI G_wGCw'C Cuts at: 0 1590 2969 3199 3500 3963 Size: 1590 1379 230 301 463 Fragments arranged by size: 1590 1379 463 301 230 BsmI GAATG_Cn' Cuts at: 0 438 1918 2466 3963 Size: 438 1480 548 1497 Fragments arranged by size: 1497 1480 548 438 Bsp1286I G_dGCh'C Cuts at: 0 924 1590 2864 2969 3199 3500 3963 Size: 924 666 1274 105 230 301 463 Fragments arranged by size: 1274 924 666 463 301 230 105 BspHI T'CATG_A Cuts at: 0 3173 3963 Size: 3173 790 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 204 3963 Size: 204 3759 BsrBI CCG'CTC Cuts at: 0 54 3963 Size: 54 3909 BsrDI GCAATG_nn' Cuts at: 0 1463 1559 1779 1913 1932 3888 3930 3963 Size: 1463 96 220 134 19 1956 42 33 Fragments arranged by size: 1956 1463 220 134 96 42 33 19 BsrFI r'CCGG_y Cuts at: 0 17 121 1571 1579 3626 3963 Size: 17 104 1450 8 2047 337 Fragments arranged by size: 2047 1450 337 104 17 8 BssHII G'CGCG_C Cuts at: 0 29 3963 Size: 29 3934 BstAPI GCAn_nnn'nTGC Cuts at: 0 219 3963 Size: 219 3744 BstBI TT'CG_AA Cuts at: 0 3486 3963 Size: 3486 477 BstEII G'GTnAC_C Cuts at: 0 249 1995 3963 Size: 249 1746 1968 Fragments arranged by size: 1968 1746 249 BstXI CCAn_nnnn'nTGG Cuts at: 0 1098 1147 1311 2482 3963 Size: 1098 49 164 1171 1481 Fragments arranged by size: 1481 1171 1098 164 49 BstYI r'GATC_y Cuts at: 0 360 402 949 1731 2242 2586 3963 Size: 360 42 547 782 511 344 1377 Fragments arranged by size: 1377 782 547 511 360 344 42 BtgI C'CryG_G Cuts at: 0 107 1140 1310 2864 3963 Size: 107 1033 170 1554 1099 Fragments arranged by size: 1554 1099 1033 170 107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 580 733 3734 3963 Size: 580 153 3001 229 Fragments arranged by size: 3001 580 229 153 BtsI GCAGTG_nn' Cuts at: 0 655 1129 1152 1552 2065 3282 3963 Size: 655 474 23 400 513 1217 681 Fragments arranged by size: 1217 681 655 513 474 400 23 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 808 843 3572 3607 3963 Size: 808 35 2729 35 356 Fragments arranged by size: 2729 808 356 35 35 EaeI y'GGCC_r Cuts at: 0 2264 2707 3854 3963 Size: 2264 443 1147 109 Fragments arranged by size: 2264 1147 443 109 EarI CTCTTCn'nnn_ Cuts at: 0 3657 3963 Size: 3657 306 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1037 3963 Size: 1037 2926 EcoICRI GAG'CTC Cuts at: 0 1588 3197 3963 Size: 1588 1609 766 Fragments arranged by size: 1609 1588 766 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 1065 1537 3096 3963 Size: 307 758 472 1559 867 Fragments arranged by size: 1559 867 758 472 307 EcoNI CCTnn'n_nnAGG Cuts at: 0 1533 3963 Size: 1533 2430 EcoO109I rG'GnC_Cy Cuts at: 0 216 3895 3963 Size: 216 3679 68 Fragments arranged by size: 3679 216 68 EcoRI G'AATT_C Cuts at: 0 547 3963 Size: 547 3416 EcoRV GAT'ATC Cuts at: 0 278 3963 Size: 278 3685 HaeII r_GCGC'y Cuts at: 0 70 107 115 173 3469 3963 Size: 70 37 8 58 3296 494 Fragments arranged by size: 3296 494 70 58 37 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 317 349 399 431 546 578 729 761 Size: 317 32 50 32 115 32 151 32 Cuts at: 761 1417 1449 1716 1748 2055 2087 2606 2638 Size: 656 32 267 32 307 32 519 32 Cuts at: 2638 2911 2943 3539 3571 3887 3919 3963 Size: 273 32 596 32 316 32 44 Fragments arranged by size: 656 596 519 317 316 307 273 267 151 115 50 44 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 698 1461 3216 3963 Size: 698 763 1755 747 Fragments arranged by size: 1755 763 747 698 HindIII A'AGCT_T Cuts at: 0 1184 1628 1903 1933 3108 3312 3963 Size: 1184 444 275 30 1175 204 651 Fragments arranged by size: 1184 1175 651 444 275 204 30 KasI G'GCGC_C Cuts at: 0 103 169 3465 3963 Size: 103 66 3296 498 Fragments arranged by size: 3296 498 103 66 KpnI G_GTAC'C Cuts at: 0 3911 3963 Size: 3911 52 MscI TGG'CCA Cuts at: 0 3856 3963 Size: 3856 107 MslI CAynn'nnrTG Cuts at: 0 679 820 1145 1660 2392 3137 3261 3963 Size: 679 141 325 515 732 745 124 702 Fragments arranged by size: 745 732 702 679 515 325 141 124 MspA1I CmG'CkG Cuts at: 0 42 109 1758 3808 3963 Size: 42 67 1649 2050 155 Fragments arranged by size: 2050 1649 155 67 42 NaeI GCC'GGC Cuts at: 0 19 123 3628 3963 Size: 19 104 3505 335 Fragments arranged by size: 3505 335 104 19 NarI GG'CG_CC Cuts at: 0 104 170 3466 3963 Size: 104 66 3296 497 Fragments arranged by size: 3296 497 104 66 NcoI C'CATG_G Cuts at: 0 1140 3963 Size: 1140 2823 NgoMIV G'CCGG_C Cuts at: 0 17 121 3626 3963 Size: 17 104 3505 337 Fragments arranged by size: 3505 337 104 17 NheI G'CTAG_C Cuts at: 0 1575 3963 Size: 1575 2388 NsiI A_TGCA'T Cuts at: 0 596 3963 Size: 596 3367 NspI r_CATG'y Cuts at: 0 704 819 2391 2799 3963 Size: 704 115 1572 408 1164 Fragments arranged by size: 1572 1164 704 408 115 PasI CC'CwG_GG Cuts at: 0 3330 3963 Size: 3330 633 PflMI CCAn_nnn'nTGG Cuts at: 0 792 1686 1948 3963 Size: 792 894 262 2015 Fragments arranged by size: 2015 894 792 262 PfoI T'CCnGG_A Cuts at: 0 1158 3963 Size: 1158 2805 PmlI CAC'GTG Cuts at: 0 3462 3963 Size: 3462 501 PpuMI rG'GwC_Cy Cuts at: 0 216 3895 3963 Size: 216 3679 68 Fragments arranged by size: 3679 216 68 PspXI vC'TCGA_Gb Cuts at: 0 3651 3963 Size: 3651 312 PstI C_TGCA'G Cuts at: 0 662 1124 1534 2679 3366 3963 Size: 662 462 410 1145 687 597 Fragments arranged by size: 1145 687 662 597 462 410 PvuII CAG'CTG Cuts at: 0 1758 3808 3963 Size: 1758 2050 155 Fragments arranged by size: 2050 1758 155 SacI G_AGCT'C Cuts at: 0 1590 3199 3963 Size: 1590 1609 764 Fragments arranged by size: 1609 1590 764 SacII CC_GC'GG Cuts at: 0 110 3963 Size: 110 3853 SanDI GG'GwC_CC Cuts at: 0 3895 3963 Size: 3895 68 SapI GCTCTTCn'nnn_ Cuts at: 0 3657 3963 Size: 3657 306 ScaI AGT'ACT Cuts at: 0 1042 2519 3963 Size: 1042 1477 1444 Fragments arranged by size: 1477 1444 1042 SexAI A'CCwGG_T Cuts at: 0 634 3963 Size: 634 3329 SfcI C'TryA_G Cuts at: 0 658 1120 1530 2675 3362 3678 3963 Size: 658 462 410 1145 687 316 285 Fragments arranged by size: 1145 687 658 462 410 316 285 SfoI GGC'GCC Cuts at: 0 105 171 3467 3963 Size: 105 66 3296 496 Fragments arranged by size: 3296 496 105 66 SgrAI Cr'CCGG_yG Cuts at: 0 17 3963 Size: 17 3946 SmlI C'TyrA_G Cuts at: 0 506 2374 2854 3501 3651 3963 Size: 506 1868 480 647 150 312 Fragments arranged by size: 1868 647 506 480 312 150 SpeI A'CTAG_T Cuts at: 0 3778 3963 Size: 3778 185 SspI AAT'ATT Cuts at: 0 939 1617 3963 Size: 939 678 2346 Fragments arranged by size: 2346 939 678 StyI C'CwwG_G Cuts at: 0 1140 1421 2747 2954 3963 Size: 1140 281 1326 207 1009 Fragments arranged by size: 1326 1140 1009 281 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1153 1626 2144 2272 3273 3963 Size: 1153 473 518 128 1001 690 Fragments arranged by size: 1153 1001 690 518 473 128 TatI w'GTAC_w Cuts at: 0 1040 2517 2798 3692 3963 Size: 1040 1477 281 894 271 Fragments arranged by size: 1477 1040 894 281 271 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 622 654 889 921 3963 Size: 622 32 235 32 3042 Fragments arranged by size: 3042 622 235 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 371 985 1620 3963 Size: 371 614 635 2343 Fragments arranged by size: 2343 635 614 371 XhoI C'TCGA_G Cuts at: 0 3651 3963 Size: 3651 312 Enzymes that do cut: AccI Acc65I AcuI AfeI AflII AflIII AhdI AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI PflMI PfoI PmlI PpuMI PspXI PstI PvuII SacI SacII SanDI SapI ScaI SexAI SfcI SfoI SgrAI SmlI SpeI SspI StyI TaqII TatI TstI XcmI XhoI Enzymes that do not cut: AarI AatII AclI AgeI AloI ApaI AscI AseI AsiSI AvrII BaeI BcgI BglII BmrI BplI BpmI BsaI BsaWI BsiEI BsiWI BsmBI BspEI BsrGI BssSI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI FalI FseI FspI FspAI HpaI MfeI MluI MmeI NdeI NotI NruI PacI PciI PmeI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SalI SbfI SfiI SgfI SmaI SnaBI SphI SrfI StuI SwaI Tth111I XbaI XmaI XmnI ZraI