################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraI DraII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MseI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tru9I Tth111I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 44 CGCG CG^CG AccIII 977 TCCGGA T^CCGGA AfaI 437 GTAC GT^AC Asp700I 1132 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 7 CTCGAG C^[CT]CG[GA]G BanI 1059 GGCACC G^G[CT][GA]CC BglII 1608 AGATCT A^GATCT BsaWI 977 TCCGGA [AT]^CCGG[AT] BseAI 977 TCCGGA T^CCGGA BsgI 123 GTGCAG GTGCAG(16/14) BsmBI 193 CGTCTC CGTCTC(1/5) BsoBI 7 CTCGAG C^[CT]CG[GA]G BspEI 977 TCCGGA T^CCGGA BsrBI 1316 GAGCGG CCGCTC(-3/-3) BsrDI 1728 GCAATG GCAATG(2/0) BstUI 44 CGCG CG^CG DraIII 1336 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1112 GACTTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1694 CCATGG C^C[GA][CT]GG EcoRV 1438 GATATC GAT^ATC EcoT14I 1694 CCATGG C^C[AT][AT]GG HgaI 45 GCGTC GACGC(5/10) HindIII 1595 AAGCTT A^AGCTT MroI 977 TCCGGA T^CCGGA MslI 792 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 44 CGCG CG^CG NcoI 1694 CCATGG C^CATGG NspI 1379 ACATGC [GA]CATG^[CT] PaeR7I 7 CTCGAG C^TCGAG PflMI 1526 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1667 GACTC GAGTC(4/5) PshAI 1692 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 437 GTAC GT^AC SacI 4 GAGCTC GAGCT^C SmlI 7 CTCGAG C^T[CT][GA]AG SspI 949 AATATT AAT^ATT StyI 1694 CCATGG C^C[AT][AT]GG Van91I 1526 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1611 TCTAGA T^CTAGA XhoI 7 CTCGAG C^TCGAG XmnI 1132 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 191 GACGTC GACGT^C 1429 GACGTC AccI 925 GTATAC GT^[AC][GT]AC 1086 GTATAC AcsI 244 GAATTC [GA]^AATT[CT] 710 GAATTC AcyI 191 GACGTC G[GA]^CG[CT]C 1429 GACGTC ApoI 244 GAATTC [GA]^AATT[CT] 710 GAATTC AspHI 4 GAGCTC G[AT]GC[AT]^C 787 GTGCTC AvaII 288 GGACC G^G[AT]CC 1698 GGTCC BalI 20 TGGCCA TGG^CCA 998 TGGCCA BanII 4 GAGCTC G[GA]GC[CT]^C 1387 GAGCCC BbsI 188 GAAGAC GAAGAC(2/6) 1489 GAAGAC BpuAI 188 GAAGAC GAAGAC(2/6) 1489 GAAGAC BsaHI 191 GACGTC G[GA]^CG[CT]C 1429 GACGTC BsiHKAI 4 GAGCTC G[AT]GC[AT]^C 787 GTGCTC BsrI 120 CCAGT ACTGG(1/-1) 1703 CCAGT Bst1107I 925 GTATAC GTA^TAC 1086 GTATAC BstZ17I 925 GTATAC GTA^TAC 1086 GTATAC CfoI 43 GCGC GCG^C 605 GCGC Cfr13I 288 GGACC G^G[ACGT]CC 1698 GGTCC EaeI 20 TGGCCA [CT]^GGCC[GA] 998 TGGCCA EcoRI 244 GAATTC G^AATTC 710 GAATTC HaeIII 21 GGCC GG^CC 999 GGCC HhaI 43 GCGC GCG^C 605 GCGC Hin1I 191 GACGTC G[GA]^CG[CT]C 1429 GACGTC HinP1I 43 GCGC G^CGC 605 GCGC MluNI 20 TGGCCA TGG^CCA 998 TGGCCA MscI 20 TGGCCA TGG^CCA 998 TGGCCA Sau96I 288 GGACC G^G[ACGT]CC 1698 GGTCC Tsp45I 170 GTGAC ^GT[GC]AC 376 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AlwI BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EarI EcoRII Fnu4HI FokI HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI Sau3AI ScrFI SfaNI SfcI TaqI TfiI TseI Tsp509I TspRI TthHB8I XhoII