(Circular) (Six-base) MAPSORT of: pFN21AE2003.seq Check: 5217 from: 1 to: 6439 pFN21AE2003 6439 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2200 3438 4249 278 Size: 53 83 186 936 664 1238 811 2468 Fragments arranged by size: 2468 1238 936 811 664 186 83 53 AccI GT'mk_AC Cuts at: 1937 2931 3092 3782 1937 Size: 994 161 690 4594 Fragments arranged by size: 4594 994 690 161 Acc65I G'GTAC_C Cuts at: 3760 3760 Size: 6439 AclI AA'CG_TT Cuts at: 1563 4566 4939 1563 Size: 3003 373 3063 Fragments arranged by size: 3063 3003 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2188 2423 3090 3349 3661 4502 5886 6154 Size: 556 235 667 259 312 841 1384 268 Cuts at: 6154 1632 Size: 1917 Fragments arranged by size: 1917 1384 841 667 556 312 268 259 235 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6242 AflIII A'CryG_T Cuts at: 1203 5338 6315 1203 Size: 4135 977 1327 Fragments arranged by size: 4135 1327 977 AgeI A'CCGG_T Cuts at: 5270 5270 Size: 6439 AhdI GACnn_n'nnGTC Cuts at: 5168 5168 Size: 6439 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6439 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6439 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3015 3047 3015 Size: 32 6407 AlwNI CAG_nnn'CTG Cuts at: 4182 5754 4182 Size: 1572 4867 ApaLI G'TGCA_C Cuts at: 4496 5652 4496 Size: 1156 5283 ApoI r'AATT_y Cuts at: 1493 2249 2715 3748 3974 4126 6235 1493 Size: 756 466 1033 226 152 2109 1697 Fragments arranged by size: 2109 1697 1033 756 466 226 152 AseI AT'TA_AT Cuts at: 160 1032 4993 160 Size: 872 3961 1606 Fragments arranged by size: 3961 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 AvaI C'yCGr_G Cuts at: 1870 1943 2012 3764 1870 Size: 73 69 1752 4545 Fragments arranged by size: 4545 1752 73 69 BamHI G'GATC_C Cuts at: 3769 4136 3769 Size: 367 6072 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3064 3760 5215 618 Size: 325 217 627 1277 696 1455 1842 Fragments arranged by size: 1842 1455 1277 696 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 3396 3758 1391 Size: 622 1383 362 4072 Fragments arranged by size: 4072 1383 622 362 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2200 3501 928 Size: 971 301 1301 3866 Fragments arranged by size: 3866 1301 971 301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4630 4664 1903 Size: 34 2693 34 3678 Fragments arranged by size: 3678 2693 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4331 5541 4331 Size: 1210 5229 BclI T'GATC_A Cuts at: 1548 1548 Size: 6439 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5050 136 Size: 107 122 71 631 605 3378 1525 Fragments arranged by size: 3378 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3613 6434 3613 Size: 2821 3618 BlpI GC'TnA_GC Cuts at: 3856 3856 Size: 6439 Bme1580I G_kGCm'C Cuts at: 1790 4500 5656 1790 Size: 2710 1156 2573 Fragments arranged by size: 2710 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6439 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3702 4170 5128 Size: 416 53 83 399 24 2285 468 958 Cuts at: 5128 442 Size: 1753 Fragments arranged by size: 2285 1753 958 468 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6439 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3472 3472 Size: 6439 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2355 2741 3395 3410 5099 1239 Size: 1116 386 654 15 1689 2579 Fragments arranged by size: 2579 1689 1116 654 386 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4564 5429 5727 4564 Size: 865 298 5276 Fragments arranged by size: 5276 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5102 882 Size: 617 3603 2219 Fragments arranged by size: 3603 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4230 493 Size: 711 3026 2702 Fragments arranged by size: 3026 2702 711 BsaBI GATnn'nnATC Cuts at: 6433 6433 Size: 6439 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2197 3435 4246 4628 Size: 53 83 186 936 664 1238 811 382 Cuts at: 4628 5263 275 Size: 635 1451 Fragments arranged by size: 1451 1238 936 811 664 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2982 4872 5270 5544 5691 6102 2982 Size: 1890 398 274 147 411 3319 Fragments arranged by size: 3319 1890 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2098 2128 1178 Size: 30 165 30 695 30 5489 Fragments arranged by size: 5489 695 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2423 3206 3239 1186 Size: 1237 783 33 4386 Fragments arranged by size: 4386 1237 783 33 BseYI C'CCAG_C Cuts at: 3301 3326 5642 6138 3301 Size: 25 2316 496 3602 Fragments arranged by size: 3602 2316 496 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2149 1163 Size: 573 413 5453 Fragments arranged by size: 5453 573 413 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3889 4650 5678 1540 Size: 275 186 1888 761 1028 2301 Fragments arranged by size: 2301 1888 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 2796 4500 4585 5656 2013 Size: 783 1704 85 1071 2796 Fragments arranged by size: 2796 1704 1071 783 85 BsmI GAATG_Cn' Cuts at: 3950 4043 3950 Size: 93 6346 BsmBI CGTCTCn'nnnn_ Cuts at: 2204 2204 Size: 6439 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2796 3396 3758 4500 4585 5656 Size: 399 223 783 600 362 742 85 1071 Cuts at: 5656 1391 Size: 2174 Fragments arranged by size: 2174 1071 783 742 600 399 362 223 85 BspEI T'CCGG_A Cuts at: 2982 6102 2982 Size: 3120 3319 BspHI T'CATG_A Cuts at: 4328 6058 4328 Size: 1730 4709 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3794 844 Size: 2950 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3323 4326 1394 Size: 429 1500 1003 3507 Fragments arranged by size: 3507 1500 1003 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3740 4934 5108 65 Size: 1183 2492 1194 174 1396 Fragments arranged by size: 2492 1396 1194 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5083 5270 1700 Size: 110 3273 187 2869 Fragments arranged by size: 3273 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6439 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6439 BssSI C'ACGA_G Cuts at: 4499 5511 4499 Size: 1012 5427 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6439 BstBI TT'CG_AA Cuts at: 5243 5243 Size: 6439 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6439 BstYI r'GATC_y Cuts at: 1972 3012 3471 3613 3769 4136 4521 4538 5990 Size: 1040 459 142 156 367 385 17 1452 Cuts at: 5990 6076 6088 6434 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1040 459 385 367 346 156 142 86 17 12 BstZ17I GTA'TAC Cuts at: 2932 3093 2932 Size: 161 6278 BtgI C'CryG_G Cuts at: 513 1065 2004 3699 513 Size: 552 939 1695 3253 Fragments arranged by size: 3253 1695 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5700 Fragments arranged by size: 5700 683 56 BtsI GCAGTG_nn' Cuts at: 2108 2137 2268 3955 4749 4769 2108 Size: 29 131 1687 794 20 3778 Fragments arranged by size: 3778 1687 794 131 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6439 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2056 2091 529 Size: 35 1492 35 4877 Fragments arranged by size: 4877 1492 35 35 DraI TTT'AAA Cuts at: 3743 3743 Size: 6439 DraIII CAC_nnn'GTG Cuts at: 3346 3346 Size: 6439 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3123 5446 809 Size: 507 1807 2323 1802 Fragments arranged by size: 2323 1807 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 3003 3886 Size: 54 1425 50 275 18 195 978 883 Cuts at: 3886 4775 6404 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 978 889 883 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3886 1537 Size: 275 2074 4090 Fragments arranged by size: 4090 2074 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2207 2672 3034 3676 3680 4369 1353 Size: 854 465 362 642 4 689 3423 Fragments arranged by size: 3423 854 689 642 465 362 4 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2607 5026 5400 5546 387 Size: 1064 138 1018 2419 374 146 1280 Fragments arranged by size: 2419 1280 1064 1018 374 146 138 EcoICRI GAG'CTC Cuts at: 2011 2011 Size: 6439 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2188 2355 2423 2741 3090 3349 3395 Size: 393 556 167 68 318 349 259 46 Cuts at: 3395 3410 3661 4502 5099 5886 6154 1239 Size: 15 251 841 597 787 268 1524 Fragments arranged by size: 1524 841 787 597 556 393 349 318 268 259 251 167 68 46 15 EcoRI G'AATT_C Cuts at: 2249 2715 3748 4126 2249 Size: 466 1033 378 4562 Fragments arranged by size: 4562 1033 466 378 EcoRV GAT'ATC Cuts at: 3445 3445 Size: 6439 FspI TGC'GCA Cuts at: 4945 4945 Size: 6439 HaeII r_GCGC'y Cuts at: 1678 5586 1678 Size: 3908 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2098 2130 2301 2333 3242 Size: 32 135 32 526 32 171 32 909 Cuts at: 3242 3274 5080 5112 5154 5186 1373 Size: 32 1806 32 42 32 2626 Fragments arranged by size: 2626 1806 909 526 171 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3783 4029 669 Size: 988 281 1845 246 3079 Fragments arranged by size: 3079 1845 988 281 246 HindIII A'AGCT_T Cuts at: 748 3600 748 Size: 2852 3587 HpaI GTT'AAC Cuts at: 4029 4029 Size: 6439 KpnI G_GTAC'C Cuts at: 3764 3764 Size: 6439 MfeI C'AATT_G Cuts at: 4038 4038 Size: 6439 MluI A'CGCG_T Cuts at: 6315 6315 Size: 6439 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3025 5553 5737 1305 Size: 1720 2528 184 2007 Fragments arranged by size: 2528 2007 1720 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 3005 10 Size: 54 1425 343 195 978 3444 Fragments arranged by size: 3444 1425 978 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2801 4397 4756 4915 518 Size: 648 1635 1596 359 159 2042 Fragments arranged by size: 2042 1635 1596 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3855 4532 5680 5925 1606 Size: 2249 677 1148 245 2120 Fragments arranged by size: 2249 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3699 513 Size: 552 939 1695 3253 Fragments arranged by size: 3253 1695 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6439 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6439 NotI GC'GGCC_GC Cuts at: 3886 3886 Size: 6439 NspI r_CATG'y Cuts at: 1132 1229 3388 3797 5342 1132 Size: 97 2159 409 1545 2229 Fragments arranged by size: 2229 2159 1545 409 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6439 PciI A'CATG_T Cuts at: 5338 5338 Size: 6439 PflMI CCAn_nnn'nTGG Cuts at: 1108 3537 1108 Size: 2429 4010 PfoI T'CCnGG_A Cuts at: 3438 3438 Size: 6439 PmeI GTTT'AAAC Cuts at: 3743 3743 Size: 6439 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4508 4540 6021 6053 4508 Size: 32 1481 32 4894 Fragments arranged by size: 4894 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3701 1613 Size: 2088 4351 PsiI TTA'TAA Cuts at: 4009 4009 Size: 6439 PstI C_TGCA'G Cuts at: 830 2887 3269 3311 3791 830 Size: 2057 382 42 480 3478 Fragments arranged by size: 3478 2057 480 382 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6439 SacI G_AGCT'C Cuts at: 2013 2013 Size: 6439 SalI G'TCGA_C Cuts at: 1936 3781 1936 Size: 1845 4594 SbfI CC_TGCA'GG Cuts at: 3791 3791 Size: 6439 ScaI AGT'ACT Cuts at: 4687 6399 4687 Size: 1712 4727 SfcI C'TryA_G Cuts at: 826 1045 2883 3265 3307 3667 3787 4922 5603 Size: 219 1838 382 42 360 120 1135 681 Cuts at: 5603 5794 826 Size: 191 1471 Fragments arranged by size: 1838 1471 1135 681 382 360 219 191 120 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6439 SmaI CCC'GGG Cuts at: 1872 3766 1872 Size: 1894 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 4543 5444 5706 820 Size: 197 926 69 2531 901 262 1553 Fragments arranged by size: 2531 1553 926 901 262 197 69 SnaBI TAC'GTA Cuts at: 493 4230 493 Size: 3737 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6439 SphI G_CATG'C Cuts at: 1132 3797 1132 Size: 2665 3774 SspI AAT'ATT Cuts at: 5 52 2956 4363 5 Size: 47 2904 1407 2081 Fragments arranged by size: 2904 2081 1407 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6439 StyI C'CwwG_G Cuts at: 513 1065 2004 3699 3878 513 Size: 552 939 1695 179 3074 Fragments arranged by size: 3074 1695 939 552 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2310 3552 4466 4483 4636 4821 5266 Size: 1255 730 1242 914 17 153 185 445 Cuts at: 5266 325 Size: 1498 Fragments arranged by size: 1498 1255 1242 914 730 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2441 4685 6397 Size: 274 80 33 51 1443 464 2244 1712 Cuts at: 6397 96 Size: 138 Fragments arranged by size: 2244 1712 1443 464 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3271 3303 6399 6431 3271 Size: 32 3096 32 3279 Fragments arranged by size: 3279 3096 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6439 XbaI T'CTAG_A Cuts at: 3616 3775 3616 Size: 159 6280 XhoI C'TCGA_G Cuts at: 1943 2012 1943 Size: 69 6370 XmaI C'CCGG_G Cuts at: 1870 3764 1870 Size: 1894 4545 XmnI GAAnn'nnTTC Cuts at: 3141 4568 3141 Size: 1427 5012 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2198 3436 4247 276 Size: 53 83 186 936 664 1238 811 2468 Fragments arranged by size: 2468 1238 936 811 664 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI Bsu36I EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI