Restriction map for
pFN21AE5179
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FbaI FokI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MaeI MaeII MamI MboI
MfeI MflI MluI MluNI MscI
MslI MspA1I MunI MvnI NaeI
NarI NciI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TseI Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 53 TCCGGA T^CCGGA
AfaI 315 GTAC GT^AC
AluI 75 AGCT AG^CT
ApaI 227 GGGCCC GGGCC^C
AspHI 67 GAGCAC G[AT]GC[AT]^C
AvaII 17 GGTCC G^G[AT]CC
BanII 227 GGGCCC G[GA]GC[CT]^C
BsaI 49 GGTCTC GGTCTC(1/5)
BsaWI 53 TCCGGA [AT]^CCGG[AT]
BseAI 53 TCCGGA T^CCGGA
BsiHKAI 67 GAGCAC G[AT]GC[AT]^C
BsmAI 50 GTCTC GTCTC(1/5)
BspEI 53 TCCGGA T^CCGGA
BssKI 178 CCTGG ^CC[ACGT]GG
BstAPI 131 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 178 CCTGG CC^[AT]GG
Cac8I 34 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 140 GCGC GCG^C
DdeI 281 CTAAG C^T[ACGT]AG
DsaI 231 CCATGG C^C[GA][CT]GG
EarI 77 CTCTTC CTCTTC(1/4)
EcoRI 118 GAATTC G^AATTC
EcoRII 178 CCTGG ^CC[AT]GG
FauI 45 GCGGG CCCGC(4/6)
Fnu4HI 182 GCGGC GC^[ACGT]GC
HapII 54 CCGG C^CGG
HhaI 140 GCGC GCG^C
HinP1I 140 GCGC G^CGC
HpaII 54 CCGG C^CGG
ItaI 182 GCGGC GC^[ACGT]GC
Ksp632I 77 CTCTTC CTCTTC(1/4)
MnlI 320 CCTC CCTC(7/6)
MroI 53 TCCGGA T^CCGGA
MseI 83 TTAA T^TAA
MspI 54 CCGG C^CGG
MvaI 178 CCTGG CC^[AT]GG
NcoI 231 CCATGG C^CATGG
PleI 203 GAGTC GAGTC(4/5)
RsaI 315 GTAC GT^AC
SapI 76 GCTCTTC GCTCTTC(1/4)
ScrFI 178 CCTGG CC^[ACGT]GG
SmlI 321 CTCAAG C^T[CT][GA]AG
TfiI 299 GAATC G^A[AT]TC
Tru9I 83 TTAA T^TAA
Tsp45I 172 GTCAC ^GT[GC]AC
TspRI 240 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 231 CCATGGAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 45 GCGG CCGC(-3/-1)
182 GCGG
AcsI 118 GAATTC [GA]^AATT[CT]
259 AAATTC
ApoI 118 GAATTC [GA]^AATT[CT]
259 AAATTC
AvaI 104 CCCGAG C^[CT]CG[GA]G
224 CTCGGG
BmyI 67 GAGCAC G[AGT]GC[ACT]^C
227 GGGCCC
BsaJI 20 CCAAGG C^C[ACGT][ACGT]GG
231 CCATGG
BsoBI 104 CCCGAG C^[CT]CG[GA]G
224 CTCGGG
Bsp1286I 67 GAGCAC G[AGT]GC[ACT]^C
227 GGGCCC
EcoT14I 20 CCAAGG C^C[AT][AT]GG
231 CCATGG
HinfI 203 GAGTC G^A[ACGT]TC
299 GAATC
MaeIII 172 GTCAC ^GT[ACGT]AC
220 GTAAC
MboII 78 TCTTC GAAGA(8/7)
273 TCTTC
MwoI 25 GCCATTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
131 GCAAGAGATGC
NlaIII 232 CATG CATG^
246 CATG
NlaIV 56 GGAACC GG[ACGT]^[ACGT]CC
227 GGGCCC
SfaNI 137 GATGC GCATC(5/9)
309 GATGC
StyI 20 CCAAGG C^C[AT][AT]GG
231 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
Cfr13I HaeIII Sau96I Tsp509I