Restriction map for
pFN21AE1698
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvrII BbeI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StyI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 224 ACATGT A^C[GA][CT]GT
AlwNI 136 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 155 CCTCAGG CC^T[ACGT]AGG
ApaI 560 GGGCCC GGGCC^C
Asp700I 33 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 82 GAGCTC G[AT]GC[AT]^C
AvaII 234 GGACC G^G[AT]CC
AviII 260 TGCGCA TGC^GCA
BamHI 571 GGATCC G^GATCC
BanI 602 GGTGCC G^G[CT][GA]CC
BbrPI 418 CACGTG CAC^GTG
BsaAI 418 CACGTG [CT]AC^GT[GA]
BsgI 452 GTGCAG GTGCAG(16/14)
BsiHKAI 82 GAGCTC G[AT]GC[AT]^C
BsmAI 591 GTCTC GTCTC(1/5)
BspLU11I 224 ACATGT A^CATGT
BstEII 525 GGTGACC G^GT[ACGT]ACC
BstPI 525 GGTGACC G^GT[ACGT]ACC
BstXI 110 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 571 GGATCC [GA]^GATC[CT]
Bsu36I 155 CCTCAGG CC^T[ACGT]AGG
DpnII 572 GATC ^GATC
Eco81I 155 CCTCAGG CC^T[ACGT]AGG
EcoNI 155 CCTCAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 525 GGTGACC G^GT[ACGT]ACC
FspI 260 TGCGCA TGC^GCA
HgaI 269 GCGTC GACGC(5/10)
HinfI 316 GAGTC G^A[ACGT]TC
MaeII 419 ACGT A^CGT
MaeIII 526 GTGAC ^GT[ACGT]AC
MboI 572 GATC ^GATC
MflI 571 GGATCC [GA]^GATC[CT]
MslI 414 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 572 GATC ^GATC
PleI 316 GAGTC GAGTC(4/5)
PmaCI 418 CACGTG CAC^GTG
PmlI 418 CACGTG CAC^GTG
PstI 643 CTGCAG CTGCA^G
SacI 82 GAGCTC GAGCT^C
Sau3AI 572 GATC ^GATC
SfcI 643 CTGCAG C^T[GA][CT]AG
StuI 467 AGGCCT AGG^CCT
TaqI 341 TCGA T^CGA
Tsp45I 526 GTGAC ^GT[GC]AC
TthHB8I 341 TCGA T^CGA
XcmI 67 CCAGCGGACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 571 GGATCC [GA]^GATC[CT]
XmnI 33 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 572 GATCC GGATC(4/5)
571 GGATC
BalI 402 TGGCCA TGG^CCA
422 TGGCCA
BbsI 37 GTCTTC GAAGAC(2/6)
271 GTCTTC
BcnI 242 CCCGG CC^[GC]GG
432 CCCGG
BglI 241 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
473 GCCAGGAGGGC
BpmI 142 CTGGAG CTGGAG(16/14)
514 CTGGAG
BpuAI 37 GTCTTC GAAGAC(2/6)
271 GTCTTC
BspMI 641 ACCTGC ACCTGC(4/8)
161 GCAGGT
DdeI 156 CTCAG C^T[ACGT]AG
658 CTCAG
DraII 344 AGGGCCT [GA]G^G[ACGT]CC[CT]
559 AGGGCCC
EaeI 402 TGGCCA [CT]^GGCC[GA]
422 TGGCCA
EcoO109I 344 AGGGCCT [GA]G^G[ACGT]CC[CT]
559 AGGGCCC
HphI 525 GGTGA GGTGA(8/7)
365 TCACC
MluNI 402 TGGCCA TGG^CCA
422 TGGCCA
MscI 402 TGGCCA TGG^CCA
422 TGGCCA
NciI 242 CCCGG CC^[GC]GG
432 CCCGG
PvuII 75 CAGCTG CAG^CTG
280 CAGCTG
SmlI 103 CTCAAG C^T[CT][GA]AG
328 CTTGAG
SphI 263 GCATGC GCATG^C
323 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AfaI AluI BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI Ksp632I MboII MnlI MspA1I
MspI MvaI MvnI MwoI NlaIII
NlaIV NspI RsaI Sau96I ScrFI
SfaNI TseI TspRI