Restriction map for
pFN21AE1698
(Linear) (Six-base) MAPSORT of: KIEE1698.seq Check: 6912 from: 1 to: 675
KIEE1698.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 675
Size: 563 112
AflIII A'CryG_T
Cuts at: 0 224 675
Size: 224 451
AlwNI CAG_nnn'CTG
Cuts at: 0 141 675
Size: 141 534
ApaI G_GGCC'C
Cuts at: 0 564 675
Size: 564 111
BamHI G'GATC_C
Cuts at: 0 571 675
Size: 571 104
BanI G'GyrC_C
Cuts at: 0 602 675
Size: 602 73
BanII G_rGCy'C
Cuts at: 0 86 256 564 675
Size: 86 170 308 111
Fragments arranged by size:
308 170 111 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 30 264 675
Size: 30 234 411
Fragments arranged by size:
411 234 30
BglI GCCn_nnn'nGGC
Cuts at: 0 247 479 675
Size: 247 232 196
Fragments arranged by size:
247 232 196
Bme1580I G_kGCm'C
Cuts at: 0 564 675
Size: 564 111
BmrI ACTGGGnnnn_n'
Cuts at: 0 104 675
Size: 104 571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 535 675
Size: 163 372 140
Fragments arranged by size:
372 163 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 349 675
Size: 88 261 326
Fragments arranged by size:
326 261 88
BsaAI yAC'GTr
Cuts at: 0 420 675
Size: 420 255
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 406 532 535 675
Size: 346 60 126 3 140
Fragments arranged by size:
346 140 126 60 3
BseYI C'CCAG_C
Cuts at: 0 77 499 675
Size: 77 422 176
Fragments arranged by size:
422 176 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 675
Size: 473 202
BsiHKAI G_wGCw'C
Cuts at: 0 86 675
Size: 86 589
Bsp1286I G_dGCh'C
Cuts at: 0 86 256 564 675
Size: 86 170 308 111
Fragments arranged by size:
308 170 111 86
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 152 650 675
Size: 152 498 25
Fragments arranged by size:
498 152 25
BstEII G'GTnAC_C
Cuts at: 0 525 675
Size: 525 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 675
Size: 117 558
BstYI r'GATC_y
Cuts at: 0 571 675
Size: 571 104
Bsu36I CC'TnA_GG
Cuts at: 0 156 675
Size: 156 519
BtsI GCAGTG_nn'
Cuts at: 0 168 388 675
Size: 168 220 287
Fragments arranged by size:
287 220 168
EaeI y'GGCC_r
Cuts at: 0 402 422 675
Size: 402 20 253
Fragments arranged by size:
402 253 20
EarI CTCTTCn'nnn_
Cuts at: 0 223 599 613 675
Size: 223 376 14 62
Fragments arranged by size:
376 223 62 14
EcoICRI GAG'CTC
Cuts at: 0 84 675
Size: 84 591
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 535 563 675
Size: 163 372 28 112
Fragments arranged by size:
372 163 112 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 159 675
Size: 159 516
EcoO109I rG'GnC_Cy
Cuts at: 0 345 560 675
Size: 345 215 115
Fragments arranged by size:
345 215 115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 54 675
Size: 54 621
FspI TGC'GCA
Cuts at: 0 262 675
Size: 262 413
FspAI rTGC'GCAy
Cuts at: 0 262 675
Size: 262 413
MscI TGG'CCA
Cuts at: 0 404 424 675
Size: 404 20 251
Fragments arranged by size:
404 251 20
MslI CAynn'nnrTG
Cuts at: 0 418 675
Size: 418 257
MspA1I CmG'CkG
Cuts at: 0 70 77 282 440 675
Size: 70 7 205 158 235
Fragments arranged by size:
235 205 158 70 7
NspI r_CATG'y
Cuts at: 0 228 267 327 675
Size: 228 39 60 348
Fragments arranged by size:
348 228 60 39
PciI A'CATG_T
Cuts at: 0 224 675
Size: 224 451
PfoI T'CCnGG_A
Cuts at: 0 230 675
Size: 230 445
PmlI CAC'GTG
Cuts at: 0 420 675
Size: 420 255
PspOMI G'GGCC_C
Cuts at: 0 560 675
Size: 560 115
PstI C_TGCA'G
Cuts at: 0 647 675
Size: 647 28
PvuII CAG'CTG
Cuts at: 0 77 282 675
Size: 77 205 393
Fragments arranged by size:
393 205 77
SacI G_AGCT'C
Cuts at: 0 86 675
Size: 86 589
SfcI C'TryA_G
Cuts at: 0 643 675
Size: 643 32
SmlI C'TyrA_G
Cuts at: 0 103 328 675
Size: 103 225 347
Fragments arranged by size:
347 225 103
SphI G_CATG'C
Cuts at: 0 267 327 675
Size: 267 60 348
Fragments arranged by size:
348 267 60
StuI AGG'CCT
Cuts at: 0 469 675
Size: 469 206
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 74 675
Size: 74 601
XmnI GAAnn'nnTTC
Cuts at: 0 37 675
Size: 37 638
Enzymes that do cut:
AcuI AflIII AlwNI ApaI BamHI BanI BanII BbsI
BglI Bme1580I BmrI BpmI BpuEI BsaAI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BstEII BstXI BstYI Bsu36I
BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI FspAI MscI MslI MspA1I NspI PciI PfoI
PmlI PspOMI PstI PvuII SacI SfcI SmlI SphI
StuI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
ZraI