################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BbeI BbsI BbvCI BciVI BfrI BglII BlnI BpuAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AocI 712 CCTGAGG CC^T[ACGT]AGG Aor51HI 2027 AGCGCT AGC^GCT ApaI 1451 GGGCCC GGGCC^C Asp700I 1935 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1833 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 84 TGGCCA TGG^CCA BamHI 1472 GGATCC G^GATCC BbrPI 1974 CACGTG CAC^GTG BcgI 544 CGAGTGGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1895 CTAG C^TAG BglI 503 GCCGCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 353 TCCGGT [AT]^CCGG[AT] BspLU11I 1937 ACATGT A^CATGT BsrFI 1771 GCCGGC [GA]^CCGG[CT] Bst1107I 639 GTATAC GTA^TAC BstAPI 1566 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 639 GTATAC GTA^TAC Bsu36I 712 CCTGAGG CC^T[ACGT]AGG Cfr10I 1771 GCCGGC [GA]^CCGG[CT] Eco47III 2027 AGCGCT AGC^GCT Eco81I 712 CCTGAGG CC^T[ACGT]AGG EcoNI 39 CCTGTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 2027 AGCGCT [GA]GCGC^[CT] HindIII 175 AAGCTT A^AGCTT MaeI 1895 CTAG C^TAG MluNI 84 TGGCCA TGG^CCA MscI 84 TGGCCA TGG^CCA NaeI 1771 GCCGGC GCC^GGC NcoI 924 CCATGG C^CATGG NgoMIV 1771 GCCGGC G^CCGGC PflFI 1833 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1974 CACGTG CAC^GTG PmlI 1974 CACGTG CAC^GTG SacI 624 GAGCTC GAGCT^C SexAI 251 ACCAGGT A^CC[AT]GGT StuI 2043 AGGCCT AGG^CCT Tth111I 1833 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1935 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflIII 1937 ACATGT A^C[GA][CT]GT 1973 ACACGT BanI 717 GGTGCC G^G[CT][GA]CC 1872 GGTGCC BclI 587 TGATCA T^GATCA 1130 TGATCA BlpI 1366 GCTGAGC GC^T[ACGT]AGC 1601 GCTCAGC BpmI 652 CTGGAG CTGGAG(16/14) 867 CTGGAG Bpu1102I 1366 GCTGAGC GC^T[ACGT]AGC 1601 GCTCAGC BsaAI 1974 CACGTG [CT]AC^GT[GA] 2119 TACGTG BsaI 280 GAGACC GGTCTC(1/5) 2109 GGTCTC BsgI 552 GTGCAG GTGCAG(16/14) 1669 GTGCAG BsmI 631 GAATGC GAATGC(1/-1) 2071 GAATGC BsrBI 1320 CCGCTC CCGCTC(-3/-3) 1908 CCGCTC BstYI 89 AGATCC [GA]^GATC[CT] 1472 GGATCC CelII 1366 GCTGAGC GC^T[ACGT]AGC 1601 GCTCAGC CfoI 1497 GCGC GCG^C 2028 GCGC DraIII 58 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG 2153 CACAAAGTG EarI 565 CTCTTC CTCTTC(1/4) 1378 CTCTTC FauI 1319 CCCGC CCCGC(4/6) 1907 CCCGC FbaI 587 TGATCA T^GATCA 1130 TGATCA HgaI 1465 GCGTC GACGC(5/10) 1925 GCGTC HhaI 1497 GCGC GCG^C 2028 GCGC HinP1I 1497 GCGC G^CGC 2028 GCGC HincII 670 GTCAAC GT[CT]^[GA]AC 1594 GTCAAC HindII 670 GTCAAC GT[CT]^[GA]AC 1594 GTCAAC Ksp632I 565 CTCTTC CTCTTC(1/4) 1378 CTCTTC MflI 89 AGATCC [GA]^GATC[CT] 1472 GGATCC MslI 405 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1515 CACCACGGTG SmlI 1729 CTTGAG C^T[CT][GA]AG 2019 CTTGAG XhoII 89 AGATCC [GA]^GATC[CT] 1472 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccI AciI AluI AlwI AlwNI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI