(Circular) (Six-base) MAPSORT of: pFN21AE2070.seq Check: 4056 from: 1 to: 6889 pFN21AE2070 6889 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3305 3305 Size: 6889 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4699 278 Size: 53 83 186 936 3163 2468 Fragments arranged by size: 3163 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2645 3099 3558 4104 4232 1937 Size: 708 454 459 546 128 4594 Fragments arranged by size: 4594 708 546 459 454 128 Acc65I G'GTAC_C Cuts at: 4210 4210 Size: 6889 AclI AA'CG_TT Cuts at: 1563 5016 5389 1563 Size: 3453 373 3063 Fragments arranged by size: 3453 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2040 3032 3074 3762 4092 4952 6336 6604 Size: 408 992 42 688 330 860 1384 268 Cuts at: 6604 1632 Size: 1917 Fragments arranged by size: 1917 1384 992 860 688 408 330 268 42 AfeI AGC'GCT Cuts at: 4034 4034 Size: 6889 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6692 AflIII A'CryG_T Cuts at: 1203 3942 3978 5788 6765 1203 Size: 2739 36 1810 977 1327 Fragments arranged by size: 2739 1810 1327 977 36 AgeI A'CCGG_T Cuts at: 5720 5720 Size: 6889 AhdI GACnn_n'nnGTC Cuts at: 5618 5618 Size: 6889 AleI CACnn'nnGTG Cuts at: 1166 3524 1166 Size: 2358 4531 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6889 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3929 3961 3929 Size: 32 6857 AlwNI CAG_nnn'CTG Cuts at: 2263 2544 3400 4632 6204 2263 Size: 281 856 1232 1572 2948 Fragments arranged by size: 2948 1572 1232 856 281 ApaI G_GGCC'C Cuts at: 3460 3460 Size: 6889 ApaLI G'TGCA_C Cuts at: 4946 6102 4946 Size: 1156 5733 ApoI r'AATT_y Cuts at: 1493 4198 4424 4576 6685 1493 Size: 2705 226 152 2109 1697 Fragments arranged by size: 2705 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5443 160 Size: 872 4411 1606 Fragments arranged by size: 4411 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6889 AvaI C'yCGr_G Cuts at: 1870 1943 2854 3615 3916 4214 1870 Size: 73 911 761 301 298 4545 Fragments arranged by size: 4545 911 761 301 298 73 BamHI G'GATC_C Cuts at: 3477 4219 4586 3477 Size: 742 367 5780 Fragments arranged by size: 5780 742 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2722 3877 4210 5665 618 Size: 325 217 627 935 1155 333 1455 1842 Fragments arranged by size: 1842 1455 1155 935 627 333 325 217 BanII G_rGCy'C Cuts at: 1391 2633 2946 3112 3204 3378 3460 4176 4208 Size: 1242 313 166 92 174 82 716 32 Cuts at: 4208 1391 Size: 4072 Fragments arranged by size: 4072 1242 716 313 174 166 92 82 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5918 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2538 2572 5080 5114 1903 Size: 34 601 34 2508 34 3678 Fragments arranged by size: 3678 2508 601 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4781 5991 4781 Size: 1210 5679 BclI T'GATC_A Cuts at: 1548 2592 3135 1548 Size: 1044 543 5302 Fragments arranged by size: 5302 1044 543 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2514 5500 136 Size: 107 122 71 631 605 842 2986 1525 Fragments arranged by size: 2986 1525 842 631 605 122 107 71 BglII A'GATC_T Cuts at: 6884 6884 Size: 6889 BlpI GC'TnA_GC Cuts at: 3372 3607 4306 3372 Size: 235 699 5955 Fragments arranged by size: 5955 699 235 Bme1580I G_kGCm'C Cuts at: 1790 2727 2909 3460 3696 4950 6106 1790 Size: 937 182 551 236 1254 1156 2573 Fragments arranged by size: 2573 1254 1156 937 551 236 182 BmgBI CAC'GTC Cuts at: 1318 3066 1318 Size: 1748 5141 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2121 2595 4141 Size: 416 53 83 399 24 704 474 1546 Cuts at: 4141 4620 5578 442 Size: 479 958 1753 Fragments arranged by size: 1753 1546 958 704 479 474 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6889 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3931 3641 Size: 290 6599 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2678 2893 5549 1239 Size: 1439 215 2656 2579 Fragments arranged by size: 2656 2579 1439 215 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3755 4045 5014 5879 6177 3755 Size: 290 969 865 298 4467 Fragments arranged by size: 4467 969 865 298 290 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2279 4120 5552 882 Size: 617 780 1841 1432 2219 Fragments arranged by size: 2219 1841 1432 780 617 BsaAI yAC'GTr Cuts at: 493 1204 3981 4126 4680 493 Size: 711 2777 145 554 2702 Fragments arranged by size: 2777 2702 711 554 145 BsaBI GATnn'nnATC Cuts at: 6883 6883 Size: 6889 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4696 5078 5713 275 Size: 53 83 186 936 3163 382 635 1451 Fragments arranged by size: 3163 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2358 5322 5720 5994 6141 6552 2358 Size: 2964 398 274 147 411 2695 Fragments arranged by size: 2964 2695 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2486 2516 3929 3959 1178 Size: 30 165 30 1083 30 1413 30 4108 Fragments arranged by size: 4108 1413 1083 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 2465 2675 1186 Size: 1207 72 210 5400 Fragments arranged by size: 5400 1207 210 72 BseYI C'CCAG_C Cuts at: 3507 3763 3780 4035 6092 6588 3507 Size: 256 17 255 2057 496 3808 Fragments arranged by size: 3808 2057 496 256 255 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2578 3695 1163 Size: 573 842 1117 4357 Fragments arranged by size: 4357 1117 842 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4339 5100 6128 1540 Size: 275 186 2338 761 1028 2301 Fragments arranged by size: 2338 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2633 2982 3627 4950 5035 6106 2633 Size: 349 645 1323 85 1071 3416 Fragments arranged by size: 3416 1323 1071 645 349 85 BsmI GAATG_Cn' Cuts at: 2642 4082 4400 4493 2642 Size: 1440 318 93 5038 Fragments arranged by size: 5038 1440 318 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2633 2727 2909 2946 2982 3112 3204 Size: 399 843 94 182 37 36 130 92 Cuts at: 3204 3378 3460 3627 3696 4176 4208 4950 5035 Size: 174 82 167 69 480 32 742 85 Cuts at: 5035 6106 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 843 742 480 399 182 174 167 130 94 92 85 82 69 37 36 32 BspEI T'CCGG_A Cuts at: 6552 6552 Size: 6889 BspHI T'CATG_A Cuts at: 4778 6508 4778 Size: 1730 5159 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2552 2688 3305 4081 4244 844 Size: 1708 136 617 776 163 3489 Fragments arranged by size: 3489 1708 776 617 163 136 BsrBI CCG'CTC Cuts at: 1394 1823 3327 3915 4776 1394 Size: 429 1504 588 861 3507 Fragments arranged by size: 3507 1504 861 588 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5384 5558 65 Size: 1183 4136 174 1396 Fragments arranged by size: 4136 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3776 5533 5720 1700 Size: 110 1966 1757 187 2869 Fragments arranged by size: 2869 1966 1757 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6889 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6889 BssSI C'ACGA_G Cuts at: 4949 5961 4949 Size: 1012 5877 BstAPI GCAn_nnn'nTGC Cuts at: 794 3577 794 Size: 2783 4106 BstBI TT'CG_AA Cuts at: 5693 5693 Size: 6889 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6889 BstXI CCAn_nnnn'nTGG Cuts at: 2105 2656 4126 2105 Size: 551 1470 4868 Fragments arranged by size: 4868 1470 551 BstYI r'GATC_y Cuts at: 1972 2094 3477 4219 4586 4971 4988 6440 6526 Size: 122 1383 742 367 385 17 1452 86 Cuts at: 6526 6538 6884 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1383 742 385 367 346 122 86 17 12 BstZ17I GTA'TAC Cuts at: 2646 2646 Size: 6889 Bsu36I CC'TnA_GG Cuts at: 2718 2718 Size: 6889 BtgI C'CryG_G Cuts at: 513 1065 2067 2929 3389 3522 3873 513 Size: 552 1002 862 460 133 351 3529 Fragments arranged by size: 3529 1002 862 552 460 351 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6150 Fragments arranged by size: 6150 683 56 BtsI GCAGTG_nn' Cuts at: 2566 3832 4405 5199 5219 2566 Size: 1266 573 794 20 4236 Fragments arranged by size: 4236 1266 794 573 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6889 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6854 DraI TTT'AAA Cuts at: 4193 4193 Size: 6889 DraIII CAC_nnn'GTG Cuts at: 2068 4163 2068 Size: 2095 4794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5896 809 Size: 507 4580 1802 Fragments arranged by size: 4580 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2311 3774 Size: 54 1425 50 275 18 259 222 1463 Cuts at: 3774 4336 5225 6854 8 Size: 562 889 1629 43 Fragments arranged by size: 1629 1463 1425 889 562 275 259 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4336 1537 Size: 275 2524 4090 Fragments arranged by size: 4090 2524 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2576 3389 4819 1353 Size: 1223 813 1430 3423 Fragments arranged by size: 3423 1430 1223 813 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5476 5850 5996 387 Size: 1064 138 3887 374 146 1280 Fragments arranged by size: 3887 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2631 2631 Size: 6889 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2678 2893 3032 3074 3762 4092 Size: 393 408 638 215 139 42 688 330 Cuts at: 4092 4952 5549 6336 6604 1239 Size: 860 597 787 268 1524 Fragments arranged by size: 1524 860 787 688 638 597 408 393 330 268 215 139 42 EcoNI CCTnn'n_nnAGG Cuts at: 2048 2048 Size: 6889 EcoO109I rG'GnC_Cy Cuts at: 2541 2774 3397 3456 3457 3701 4002 4067 2541 Size: 233 623 59 1 244 301 65 5363 Fragments arranged by size: 5363 623 301 244 233 65 59 1 EcoRI G'AATT_C Cuts at: 4198 4576 4198 Size: 378 6511 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2203 2980 2203 Size: 777 6112 FspI TGC'GCA Cuts at: 5395 5395 Size: 6889 HaeII r_GCGC'y Cuts at: 1678 4036 6036 1678 Size: 2358 2000 2531 Fragments arranged by size: 2531 2358 2000 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2441 2473 3034 3066 5530 Size: 32 135 32 869 32 561 32 2464 Cuts at: 5530 5562 5604 5636 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2464 869 561 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2677 3601 4233 4479 669 Size: 988 281 739 924 632 246 3079 Fragments arranged by size: 3079 988 924 739 632 281 246 HindIII A'AGCT_T Cuts at: 748 2180 748 Size: 1432 5457 HpaI GTT'AAC Cuts at: 4479 4479 Size: 6889 KpnI G_GTAC'C Cuts at: 4214 4214 Size: 6889 MfeI C'AATT_G Cuts at: 4488 4488 Size: 6889 MluI A'CGCG_T Cuts at: 6765 6765 Size: 6889 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6003 6187 1305 Size: 4698 184 2007 Fragments arranged by size: 4698 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2091 10 Size: 54 1425 343 259 4808 Fragments arranged by size: 4808 1425 343 259 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 3524 4847 5206 5365 518 Size: 648 1248 1110 1323 359 159 2042 Fragments arranged by size: 2042 1323 1248 1110 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2988 3611 3679 4305 4982 6130 6375 1606 Size: 1382 623 68 626 677 1148 245 2120 Fragments arranged by size: 2120 1382 1148 677 626 623 245 68 NaeI GCC'GGC Cuts at: 3778 3778 Size: 6889 NcoI C'CATG_G Cuts at: 513 1065 2929 513 Size: 552 1864 4473 Fragments arranged by size: 4473 1864 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6889 NgoMIV G'CCGG_C Cuts at: 3776 3776 Size: 6889 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6889 NotI GC'GGCC_GC Cuts at: 4336 4336 Size: 6889 NspI r_CATG'y Cuts at: 1132 1229 3551 3763 3946 4247 5792 1132 Size: 97 2322 212 183 301 1545 2229 Fragments arranged by size: 2322 2229 1545 301 212 183 97 PasI CC'CwG_GG Cuts at: 1348 3697 1348 Size: 2349 4540 PciI A'CATG_T Cuts at: 3942 5788 3942 Size: 1846 5043 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2068 2263 2320 2410 3311 3763 1108 Size: 902 58 195 57 90 901 452 4234 Fragments arranged by size: 4234 902 901 452 195 90 58 57 PfoI T'CCnGG_A Cuts at: 3473 3473 Size: 6889 PmeI GTTT'AAAC Cuts at: 4193 4193 Size: 6889 PmlI CAC'GTG Cuts at: 3981 3981 Size: 6889 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 4958 4990 6471 6503 3929 Size: 32 997 32 1481 32 4315 Fragments arranged by size: 4315 1481 997 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2541 3701 4067 2541 Size: 1160 366 5363 Fragments arranged by size: 5363 1160 366 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6889 PsiI TTA'TAA Cuts at: 4459 4459 Size: 6889 PspOMI G'GGCC_C Cuts at: 3456 3456 Size: 6889 PstI C_TGCA'G Cuts at: 830 2685 2896 3684 3858 4241 830 Size: 1855 211 788 174 383 3478 Fragments arranged by size: 3478 1855 788 383 211 174 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6889 PvuII CAG'CTG Cuts at: 2988 3611 3679 2988 Size: 623 68 6198 Fragments arranged by size: 6198 623 68 SacI G_AGCT'C Cuts at: 2633 2633 Size: 6889 SalI G'TCGA_C Cuts at: 1936 4231 1936 Size: 2295 4594 SanDI GG'GwC_CC Cuts at: 3701 3701 Size: 6889 SbfI CC_TGCA'GG Cuts at: 4241 4241 Size: 6889 ScaI AGT'ACT Cuts at: 5137 6849 5137 Size: 1712 5177 SexAI A'CCwGG_T Cuts at: 2256 2256 Size: 6889 SfcI C'TryA_G Cuts at: 826 1045 2681 2892 2915 3103 3680 3854 4237 Size: 219 1636 211 23 188 577 174 383 Cuts at: 4237 5372 6053 6244 826 Size: 1135 681 191 1471 Fragments arranged by size: 1636 1471 1135 681 577 383 219 211 191 188 174 23 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6889 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6889 SmaI CCC'GGG Cuts at: 1872 4216 1872 Size: 2344 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3734 4024 4993 5894 6156 820 Size: 197 926 1791 290 969 901 262 1553 Fragments arranged by size: 1791 1553 969 926 901 290 262 197 SnaBI TAC'GTA Cuts at: 493 4680 493 Size: 4187 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6889 SphI G_CATG'C Cuts at: 1132 4247 1132 Size: 3115 3774 SspI AAT'ATT Cuts at: 5 52 4813 5 Size: 47 4761 2081 Fragments arranged by size: 4761 2081 47 StuI AGG'CCT Cuts at: 1517 4050 1517 Size: 2533 4356 StyI C'CwwG_G Cuts at: 513 1065 2929 3253 3540 3728 3985 4328 513 Size: 552 1864 324 287 188 257 343 3074 Fragments arranged by size: 3074 1864 552 343 324 287 257 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2828 3645 4027 4123 4916 4933 5086 Size: 1255 1248 817 382 96 793 17 153 Cuts at: 5086 5271 5716 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1248 817 793 445 382 185 153 96 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5135 6847 96 Size: 274 80 33 51 1443 3158 1712 138 Fragments arranged by size: 3158 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3050 3082 3286 3318 4121 4153 6849 6881 3050 Size: 32 204 32 803 32 2696 32 3058 Fragments arranged by size: 3058 2696 803 204 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3841 1316 Size: 2525 4364 XbaI T'CTAG_A Cuts at: 4225 4225 Size: 6889 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2483 3438 3805 2483 Size: 955 367 5567 Fragments arranged by size: 5567 955 367 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6889 XmaI C'CCGG_G Cuts at: 1870 4214 1870 Size: 2344 4545 XmnI GAAnn'nnTTC Cuts at: 3944 5018 3944 Size: 1074 5815 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4697 276 Size: 53 83 186 936 3163 2468 Fragments arranged by size: 3163 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI