(Linear) (Six-base) MAPSORT of: KIEE2070.seq Check: 3965 from: 1 to: 2184 KIEE2070.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1300 2184 Size: 1300 884 AccI GT'mk_AC Cuts at: 0 640 1094 1553 2099 2184 Size: 640 454 459 546 85 Fragments arranged by size: 640 546 459 454 85 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 1027 1069 1757 2087 2184 Size: 35 992 42 688 330 97 Fragments arranged by size: 992 688 330 97 42 35 AfeI AGC'GCT Cuts at: 0 2029 2184 Size: 2029 155 AflIII A'CryG_T Cuts at: 0 1937 1973 2184 Size: 1937 36 211 Fragments arranged by size: 1937 211 36 AleI CACnn'nnGTG Cuts at: 0 1519 2184 Size: 1519 665 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1924 1956 2184 Size: 1924 32 228 Fragments arranged by size: 1924 228 32 AlwNI CAG_nnn'CTG Cuts at: 0 258 539 1395 2184 Size: 258 281 856 789 Fragments arranged by size: 856 789 281 258 ApaI G_GGCC'C Cuts at: 0 1455 2184 Size: 1455 729 AvaI C'yCGr_G Cuts at: 0 849 1610 1911 2184 Size: 849 761 301 273 Fragments arranged by size: 849 761 301 273 BamHI G'GATC_C Cuts at: 0 1472 2184 Size: 1472 712 BanI G'GyrC_C Cuts at: 0 717 1872 2184 Size: 717 1155 312 Fragments arranged by size: 1155 717 312 BanII G_rGCy'C Cuts at: 0 628 941 1107 1199 1373 1455 2171 2184 Size: 628 313 166 92 174 82 716 13 Fragments arranged by size: 716 628 313 174 166 92 82 13 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 533 567 2184 Size: 533 34 1617 Fragments arranged by size: 1617 533 34 BclI T'GATC_A Cuts at: 0 587 1130 2184 Size: 587 543 1054 Fragments arranged by size: 1054 587 543 BglI GCCn_nnn'nGGC Cuts at: 0 509 2184 Size: 509 1675 BlpI GC'TnA_GC Cuts at: 0 1367 1602 2184 Size: 1367 235 582 Fragments arranged by size: 1367 582 235 Bme1580I G_kGCm'C Cuts at: 0 722 904 1455 1691 2184 Size: 722 182 551 236 493 Fragments arranged by size: 722 551 493 236 182 BmgBI CAC'GTC Cuts at: 0 1061 2184 Size: 1061 1123 BmrI ACTGGGnnnn_n' Cuts at: 0 116 590 2136 2184 Size: 116 474 1546 48 Fragments arranged by size: 1546 474 116 48 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1636 1926 2184 Size: 1636 290 258 Fragments arranged by size: 1636 290 258 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 673 888 2184 Size: 673 215 1296 Fragments arranged by size: 1296 673 215 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1750 2040 2184 Size: 1750 290 144 Fragments arranged by size: 1750 290 144 BsaI GGTCTCn'nnnn_ Cuts at: 0 274 2115 2184 Size: 274 1841 69 Fragments arranged by size: 1841 274 69 BsaAI yAC'GTr Cuts at: 0 1976 2121 2184 Size: 1976 145 63 Fragments arranged by size: 1976 145 63 BsaWI w'CCGG_w Cuts at: 0 353 2184 Size: 353 1831 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 481 511 1924 1954 2184 Size: 481 30 1413 30 230 Fragments arranged by size: 1413 481 230 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 388 460 670 2184 Size: 388 72 210 1514 Fragments arranged by size: 1514 388 210 72 BseYI C'CCAG_C Cuts at: 0 1502 1758 1775 2030 2184 Size: 1502 256 17 255 154 Fragments arranged by size: 1502 256 255 154 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 573 1690 2184 Size: 573 1117 494 Fragments arranged by size: 1117 573 494 BsiHKAI G_wGCw'C Cuts at: 0 628 977 1622 2184 Size: 628 349 645 562 Fragments arranged by size: 645 628 562 349 BsmI GAATG_Cn' Cuts at: 0 637 2077 2184 Size: 637 1440 107 Fragments arranged by size: 1440 637 107 Bsp1286I G_dGCh'C Cuts at: 0 628 722 904 941 977 1107 1199 1373 Size: 628 94 182 37 36 130 92 174 Cuts at: 1373 1455 1622 1691 2171 2184 Size: 82 167 69 480 13 Fragments arranged by size: 628 480 182 174 167 130 94 92 82 69 37 36 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 547 683 1300 2076 2184 Size: 547 136 617 776 108 Fragments arranged by size: 776 617 547 136 108 BsrBI CCG'CTC Cuts at: 0 1322 1910 2184 Size: 1322 588 274 Fragments arranged by size: 1322 588 274 BsrFI r'CCGG_y Cuts at: 0 1771 2184 Size: 1771 413 BstAPI GCAn_nnn'nTGC Cuts at: 0 1572 2184 Size: 1572 612 BstXI CCAn_nnnn'nTGG Cuts at: 0 100 651 2121 2184 Size: 100 551 1470 63 Fragments arranged by size: 1470 551 100 63 BstYI r'GATC_y Cuts at: 0 89 1472 2184 Size: 89 1383 712 Fragments arranged by size: 1383 712 89 BstZ17I GTA'TAC Cuts at: 0 641 2184 Size: 641 1543 Bsu36I CC'TnA_GG Cuts at: 0 713 2184 Size: 713 1471 BtgI C'CryG_G Cuts at: 0 62 924 1384 1517 1868 2184 Size: 62 862 460 133 351 316 Fragments arranged by size: 862 460 351 316 133 62 BtsI GCAGTG_nn' Cuts at: 0 561 1827 2184 Size: 561 1266 357 Fragments arranged by size: 1266 561 357 DraIII CAC_nnn'GTG Cuts at: 0 63 2158 2184 Size: 63 2095 26 Fragments arranged by size: 2095 63 26 EaeI y'GGCC_r Cuts at: 0 84 306 1769 2184 Size: 84 222 1463 415 Fragments arranged by size: 1463 415 222 84 EarI CTCTTCn'nnn_ Cuts at: 0 571 1384 2184 Size: 571 813 800 Fragments arranged by size: 813 800 571 EcoICRI GAG'CTC Cuts at: 0 626 2184 Size: 626 1558 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 673 888 1027 1069 1757 2087 2184 Size: 35 638 215 139 42 688 330 97 Fragments arranged by size: 688 638 330 215 139 97 42 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 43 2184 Size: 43 2141 EcoO109I rG'GnC_Cy Cuts at: 0 536 769 1392 1451 1452 1696 1997 2062 Size: 536 233 623 59 1 244 301 65 Cuts at: 2062 2184 Size: 122 Fragments arranged by size: 623 536 301 244 233 122 65 59 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 198 975 2184 Size: 198 777 1209 Fragments arranged by size: 1209 777 198 HaeII r_GCGC'y Cuts at: 0 2031 2184 Size: 2031 153 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 436 468 1029 1061 2184 Size: 436 32 561 32 1123 Fragments arranged by size: 1123 561 436 32 32 HincII GTy'rAC Cuts at: 0 672 1596 2184 Size: 672 924 588 Fragments arranged by size: 924 672 588 HindIII A'AGCT_T Cuts at: 0 175 2184 Size: 175 2009 MscI TGG'CCA Cuts at: 0 86 2184 Size: 86 2098 MslI CAynn'nnrTG Cuts at: 0 409 1519 2184 Size: 409 1110 665 Fragments arranged by size: 1110 665 409 MspA1I CmG'CkG Cuts at: 0 983 1606 1674 2184 Size: 983 623 68 510 Fragments arranged by size: 983 623 510 68 NaeI GCC'GGC Cuts at: 0 1773 2184 Size: 1773 411 NcoI C'CATG_G Cuts at: 0 924 2184 Size: 924 1260 NgoMIV G'CCGG_C Cuts at: 0 1771 2184 Size: 1771 413 NspI r_CATG'y Cuts at: 0 1546 1758 1941 2184 Size: 1546 212 183 243 Fragments arranged by size: 1546 243 212 183 PasI CC'CwG_GG Cuts at: 0 1692 2184 Size: 1692 492 PciI A'CATG_T Cuts at: 0 1937 2184 Size: 1937 247 PflMI CCAn_nnn'nTGG Cuts at: 0 63 258 315 405 1306 1758 2184 Size: 63 195 57 90 901 452 426 Fragments arranged by size: 901 452 426 195 90 63 57 PfoI T'CCnGG_A Cuts at: 0 1468 2184 Size: 1468 716 PmlI CAC'GTG Cuts at: 0 1976 2184 Size: 1976 208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1924 1956 2184 Size: 1924 32 228 Fragments arranged by size: 1924 228 32 PpuMI rG'GwC_Cy Cuts at: 0 536 1696 2062 2184 Size: 536 1160 366 122 Fragments arranged by size: 1160 536 366 122 PspOMI G'GGCC_C Cuts at: 0 1451 2184 Size: 1451 733 PstI C_TGCA'G Cuts at: 0 680 891 1679 1853 2184 Size: 680 211 788 174 331 Fragments arranged by size: 788 680 331 211 174 PvuII CAG'CTG Cuts at: 0 983 1606 1674 2184 Size: 983 623 68 510 Fragments arranged by size: 983 623 510 68 SacI G_AGCT'C Cuts at: 0 628 2184 Size: 628 1556 SanDI GG'GwC_CC Cuts at: 0 1696 2184 Size: 1696 488 SexAI A'CCwGG_T Cuts at: 0 251 2184 Size: 251 1933 SfcI C'TryA_G Cuts at: 0 676 887 910 1098 1675 1849 2184 Size: 676 211 23 188 577 174 335 Fragments arranged by size: 676 577 335 211 188 174 23 SmlI C'TyrA_G Cuts at: 0 1729 2019 2184 Size: 1729 290 165 Fragments arranged by size: 1729 290 165 StuI AGG'CCT Cuts at: 0 2045 2184 Size: 2045 139 StyI C'CwwG_G Cuts at: 0 924 1248 1535 1723 1980 2184 Size: 924 324 287 188 257 204 Fragments arranged by size: 924 324 287 257 204 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 823 1640 2022 2118 2184 Size: 823 817 382 96 66 Fragments arranged by size: 823 817 382 96 66 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1045 1077 1281 1313 2116 2148 2184 Size: 1045 32 204 32 803 32 36 Fragments arranged by size: 1045 803 204 36 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1836 2184 Size: 1836 348 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 478 1433 1800 2184 Size: 478 955 367 384 Fragments arranged by size: 955 478 384 367 XmnI GAAnn'nnTTC Cuts at: 0 1939 2184 Size: 1939 245 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AleI AloI AlwNI ApaI AvaI BamHI BanI BanII BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SanDI SexAI SfcI SmlI StuI StyI TaqII TstI Tth111I XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BciVI BfrBI BglII BmtI Bpu10I BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BtgZI ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XhoI XmaI ZraI