################################## # NO CUT ENZYMES ( 210 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DpnII DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MboI MboII MfeI MflI MluI MroI MslI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AfaI 8 GTAC GT^AC AseI 192 ATTAAT AT^TAAT AsnI 192 ATTAAT AT^TAAT BalI 2 TGGCCA TGG^CCA BsaBI 238 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsrDI 33 CATTGC GCAATG(2/0) BsrI 5 CCAGT ACTGG(1/-1) BstAPI 43 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 268 CATCC GGATG(2/0) BstXI 5 CCAGTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 261 TTTAAA TTT^AAA EaeI 2 TGGCCA [CT]^GGCC[GA] EcoNI 271 CCTGACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 268 CATCC GGATG(9/13) MaeII 62 ACGT A^CGT MaeIII 59 GTTAC ^GT[ACGT]AC MamI 238 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA NdeI 82 CATATG CA^TATG NlaIII 75 CATG CATG^ NlaIV 87 GGAGCC GG[ACGT]^[ACGT]CC PshBI 192 ATTAAT AT^TAAT PstI 41 CTGCAG CTGCA^G RsaI 8 GTAC GT^AC SfaNI 238 GATGC GCATC(5/9) SfcI 41 CTGCAG C^T[GA][CT]AG SmlI 92 CTCAAG C^T[CT][GA]AG TaqI 246 TCGA T^CGA Tsp509I 252 AATT ^AATT TthHB8I 246 TCGA T^CGA XcmI 74 CCATGAAGCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AluI 315 AGCT AG^CT 339 AGCT BfaI 199 CTAG C^TAG 328 CTAG BsiYI 218 CCAATAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 271 CCTGACAAAGG BslI 218 CCAATAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 271 CCTGACAAAGG Cac8I 52 GCAGGC GC[ACGT]^[ACGT]GC 69 GCAAGC HaeIII 3 GGCC GG^CC 55 GGCC MaeI 199 CTAG C^TAG 328 CTAG MseI 193 TTAA T^TAA 262 TTAA MwoI 43 GCAGGATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 81 GCATATGGAGC Tru9I 193 TTAA T^TAA 262 TTAA TspRI 11 CAGTG CA[GC]TG[ACGT][ACGT]^ 135 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### BbvI Fnu4HI ItaI MnlI TseI