(Circular) (Six-base) MAPSORT of: pFN21AE6549.seq Check: 6177 from: 1 to: 5053 pFN21AE6549 5053 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2863 278 Size: 53 83 186 936 1327 2468 Fragments arranged by size: 2468 1327 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2396 1937 Size: 459 4594 Acc65I G'GTAC_C Cuts at: 2374 2374 Size: 5053 AclI AA'CG_TT Cuts at: 1563 3180 3553 1563 Size: 1617 373 3063 Fragments arranged by size: 3063 1617 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2122 3116 4500 4768 1632 Size: 490 994 1384 268 1917 Fragments arranged by size: 1917 1384 994 490 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4856 AflIII A'CryG_T Cuts at: 1203 3952 4929 1203 Size: 2749 977 1327 Fragments arranged by size: 2749 1327 977 AgeI A'CCGG_T Cuts at: 3884 3884 Size: 5053 AhdI GACnn_n'nnGTC Cuts at: 3782 3782 Size: 5053 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5053 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5053 AlwNI CAG_nnn'CTG Cuts at: 2796 4368 2796 Size: 1572 3481 ApaLI G'TGCA_C Cuts at: 3110 4266 3110 Size: 1156 3897 ApoI r'AATT_y Cuts at: 1493 2362 2588 2740 4849 1493 Size: 869 226 152 2109 1697 Fragments arranged by size: 2109 1697 869 226 152 AseI AT'TA_AT Cuts at: 160 1032 2198 3607 160 Size: 872 1166 1409 1606 Fragments arranged by size: 1606 1409 1166 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5053 AvaI C'yCGr_G Cuts at: 1870 1943 2378 1870 Size: 73 435 4545 Fragments arranged by size: 4545 435 73 BamHI G'GATC_C Cuts at: 2383 2750 2383 Size: 367 4686 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2374 3829 618 Size: 325 217 627 587 1455 1842 Fragments arranged by size: 1842 1455 627 587 325 217 BanII G_rGCy'C Cuts at: 1391 2372 1391 Size: 981 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4082 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3244 3278 1903 Size: 34 1307 34 3678 Fragments arranged by size: 3678 1307 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2945 4155 2945 Size: 1210 3843 BclI T'GATC_A Cuts at: 1548 1548 Size: 5053 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3664 136 Size: 107 122 71 631 605 1992 1525 Fragments arranged by size: 1992 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5048 5048 Size: 5053 BlpI GC'TnA_GC Cuts at: 2470 2470 Size: 5053 Bme1580I G_kGCm'C Cuts at: 1790 3114 4270 1790 Size: 1324 1156 2573 Fragments arranged by size: 2573 1324 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5053 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2784 3742 442 Size: 416 53 83 399 24 1367 958 1753 Fragments arranged by size: 1753 1367 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5053 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3713 1239 Size: 2474 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2082 3178 4043 4341 2082 Size: 1096 865 298 2794 Fragments arranged by size: 2794 1096 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3716 882 Size: 617 2217 2219 Fragments arranged by size: 2219 2217 617 BsaAI yAC'GTr Cuts at: 493 1204 2844 493 Size: 711 1640 2702 Fragments arranged by size: 2702 1640 711 BsaBI GATnn'nnATC Cuts at: 2247 5047 2247 Size: 2800 2253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2860 3242 3877 275 Size: 53 83 186 936 1327 382 635 1451 Fragments arranged by size: 1451 1327 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3486 3884 4158 4305 4716 3486 Size: 398 274 147 411 3823 Fragments arranged by size: 3823 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4828 Fragments arranged by size: 4828 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5053 BseYI C'CCAG_C Cuts at: 4256 4752 4256 Size: 496 4557 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4480 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2503 3264 4292 1540 Size: 275 186 502 761 1028 2301 Fragments arranged by size: 2301 1028 761 502 275 186 BsiHKAI G_wGCw'C Cuts at: 3114 3199 4270 3114 Size: 85 1071 3897 Fragments arranged by size: 3897 1071 85 BsmI GAATG_Cn' Cuts at: 2564 2657 2564 Size: 93 4960 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2372 3114 3199 4270 1391 Size: 399 582 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 582 399 85 BspEI T'CCGG_A Cuts at: 4716 4716 Size: 5053 BspHI T'CATG_A Cuts at: 2942 4672 2942 Size: 1730 3323 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2408 844 Size: 1564 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2940 1394 Size: 429 1117 3507 Fragments arranged by size: 3507 1117 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2037 3548 3722 65 Size: 1183 789 1511 174 1396 Fragments arranged by size: 1511 1396 1183 789 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3697 3884 1700 Size: 110 1887 187 2869 Fragments arranged by size: 2869 1887 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5053 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5053 BssSI C'ACGA_G Cuts at: 3113 4125 3113 Size: 1012 4041 BstAPI GCAn_nnn'nTGC Cuts at: 794 2054 794 Size: 1260 3793 BstBI TT'CG_AA Cuts at: 3857 3857 Size: 5053 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5053 BstXI CCAn_nnnn'nTGG Cuts at: 2017 2017 Size: 5053 BstYI r'GATC_y Cuts at: 1972 2383 2750 3135 3152 4604 4690 4702 5048 Size: 411 367 385 17 1452 86 12 346 Cuts at: 5048 1972 Size: 1977 Fragments arranged by size: 1977 1452 411 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3562 Fragments arranged by size: 3562 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4314 Fragments arranged by size: 4314 683 56 BtsI GCAGTG_nn' Cuts at: 2569 3363 3383 2569 Size: 794 20 4239 Fragments arranged by size: 4239 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5053 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5018 DraI TTT'AAA Cuts at: 2268 2357 2268 Size: 89 4964 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4060 809 Size: 507 2744 1802 Fragments arranged by size: 2744 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2500 3389 Size: 54 1425 50 275 18 177 493 889 Cuts at: 3389 5018 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 493 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2500 1537 Size: 275 688 4090 Fragments arranged by size: 4090 688 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2983 1353 Size: 1630 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3640 4014 4160 387 Size: 1064 138 2051 374 146 1280 Fragments arranged by size: 2051 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 3116 3713 4500 4768 1239 Size: 393 490 994 597 787 268 1524 Fragments arranged by size: 1524 994 787 597 490 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2280 2280 Size: 5053 EcoRI G'AATT_C Cuts at: 2362 2740 2362 Size: 378 4675 FspI TGC'GCA Cuts at: 3559 3559 Size: 5053 HaeII r_GCGC'y Cuts at: 1678 4200 1678 Size: 2522 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3694 3726 3768 3800 1373 Size: 32 135 32 2122 32 42 32 2626 Fragments arranged by size: 2626 2122 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2397 2643 669 Size: 988 281 459 246 3079 Fragments arranged by size: 3079 988 459 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5053 HpaI GTT'AAC Cuts at: 2643 2643 Size: 5053 KpnI G_GTAC'C Cuts at: 2378 2378 Size: 5053 MfeI C'AATT_G Cuts at: 2652 2652 Size: 5053 MluI A'CGCG_T Cuts at: 4929 4929 Size: 5053 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2008 4167 4351 1305 Size: 703 2159 184 2007 Fragments arranged by size: 2159 2007 703 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3054 Fragments arranged by size: 3054 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3011 3370 3529 518 Size: 648 1845 359 159 2042 Fragments arranged by size: 2042 1845 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2469 3146 4294 4539 1606 Size: 863 677 1148 245 2120 Fragments arranged by size: 2120 1148 863 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3562 Fragments arranged by size: 3562 939 552 NdeI CA'TA_TG Cuts at: 387 2088 387 Size: 1701 3352 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5053 NotI GC'GGCC_GC Cuts at: 2500 2500 Size: 5053 NspI r_CATG'y Cuts at: 1132 1229 2411 3956 1132 Size: 97 1182 1545 2229 Fragments arranged by size: 2229 1545 1182 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5053 PciI A'CATG_T Cuts at: 3952 3952 Size: 5053 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5053 PmeI GTTT'AAAC Cuts at: 2357 2357 Size: 5053 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3122 3154 4635 4667 3122 Size: 32 1481 32 3508 Fragments arranged by size: 3508 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5053 PsiI TTA'TAA Cuts at: 2623 2623 Size: 5053 PstI C_TGCA'G Cuts at: 830 2050 2405 830 Size: 1220 355 3478 Fragments arranged by size: 3478 1220 355 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5053 SalI G'TCGA_C Cuts at: 1936 2395 1936 Size: 459 4594 SbfI CC_TGCA'GG Cuts at: 2405 2405 Size: 5053 ScaI AGT'ACT Cuts at: 3301 5013 3301 Size: 1712 3341 SfcI C'TryA_G Cuts at: 826 1045 2046 2401 3536 4217 4408 826 Size: 219 1001 355 1135 681 191 1471 Fragments arranged by size: 1471 1135 1001 681 355 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5053 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5053 SmaI CCC'GGG Cuts at: 1872 2380 1872 Size: 508 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2097 3157 4058 4320 820 Size: 197 926 154 1060 901 262 1553 Fragments arranged by size: 1553 1060 926 901 262 197 154 SnaBI TAC'GTA Cuts at: 493 2844 493 Size: 2351 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5053 SphI G_CATG'C Cuts at: 1132 2411 1132 Size: 1279 3774 SspI AAT'ATT Cuts at: 5 52 2977 5 Size: 47 2925 2081 Fragments arranged by size: 2925 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5053 StyI C'CwwG_G Cuts at: 513 1065 2004 2492 513 Size: 552 939 488 3074 Fragments arranged by size: 3074 939 552 488 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3080 3097 3250 3435 3880 325 Size: 1255 1500 17 153 185 445 1498 Fragments arranged by size: 1500 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2012 3299 5011 Size: 274 80 33 51 1443 35 1287 1712 Cuts at: 5011 96 Size: 138 Fragments arranged by size: 1712 1443 1287 274 138 80 51 35 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5013 5045 5013 Size: 32 5021 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5053 XbaI T'CTAG_A Cuts at: 2389 2389 Size: 5053 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2086 2086 Size: 5053 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5053 XmaI C'CCGG_G Cuts at: 1870 2378 1870 Size: 508 4545 XmnI GAAnn'nnTTC Cuts at: 3182 3182 Size: 5053 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2861 276 Size: 53 83 186 936 1327 2468 Fragments arranged by size: 2468 1327 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI