################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BalI BamHI BbeI BbrPI BbsI BcgI BclI BcnI BfrI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI TaqI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflIII 1208 ACATGT A^C[GA][CT]GT AocI 379 CCTCAGG CC^T[ACGT]AGG AvrII 804 CCTAGG C^CTAGG BanI 135 GGTGCC G^G[CT][GA]CC BbvCI 1927 GCTGAGG CCTCAGC(-5/-2) BfaI 805 CTAG C^TAG BglI 947 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 804 CCTAGG C^CTAGG BseRI 479 GAGGAG GAGGAG(10/8) Bsp1407I 569 TGTACA T^GTACA BspLU11I 1208 ACATGT A^CATGT BsrGI 569 TGTACA T^GTACA BssKI 391 CCTGG ^CC[ACGT]GG BstAPI 316 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1156 GGTGACC G^GT[ACGT]ACC BstNI 391 CCTGG CC^[AT]GG BstPI 1156 GGTGACC G^GT[ACGT]ACC Bsu36I 379 CCTCAGG CC^T[ACGT]AGG Cac8I 296 GCTCGC GC[ACGT]^[ACGT]GC DpnII 926 GATC ^GATC EaeI 703 CGGCCA [CT]^GGCC[GA] EarI 368 CTCTTC CTCTTC(1/4) Eco81I 379 CCTCAGG CC^T[ACGT]AGG EcoO65I 1156 GGTGACC G^GT[ACGT]ACC EcoRII 391 CCTGG ^CC[AT]GG Ksp632I 368 CTCTTC CTCTTC(1/4) MaeI 805 CTAG C^TAG MaeII 904 ACGT A^CGT MboI 926 GATC ^GATC MvaI 391 CCTGG CC^[AT]GG NdeII 926 GATC ^GATC NlaIV 135 GGTGCC GG[ACGT]^[ACGT]CC PflMI 646 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau3AI 926 GATC ^GATC ScrFI 391 CCTGG CC^[ACGT]GG SmlI 1372 CTTGAG C^T[CT][GA]AG SspBI 569 TGTACA T^GTACA SwaI 242 ATTTAAAT ATTT^AAAT Van91I 646 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AspHI 294 GAGCTC G[AT]GC[AT]^C 1270 GAGCTC AvaII 307 GGACC G^G[AT]CC 1920 GGACC BciVI 1134 GTATCC GTATCC(6/5) 1255 GTATCC BsaJI 26 CCTTGG C^C[ACGT][ACGT]GG 804 CCTAGG BsiHKAI 294 GAGCTC G[AT]GC[AT]^C 1270 GAGCTC BsrDI 398 CATTGC GCAATG(2/0) 976 CATTGC EcoT14I 26 CCTTGG C^C[AT][AT]GG 804 CCTAGG EcoT22I 913 ATGCAT ATGCA^T 1430 ATGCAT NsiI 913 ATGCAT ATGCA^T 1430 ATGCAT SacI 294 GAGCTC GAGCT^C 1270 GAGCTC StyI 26 CCTTGG C^C[AT][AT]GG 804 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BanII BbvI BmyI BpmI BsiYI BslI BsmAI BsmI Bsp1286I BsrI BstF5I Cfr13I DdeI DraI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MseI MslI MwoI NlaIII NspI PleI RsaI Sau96I SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI