(Circular) (Six-base) MAPSORT of: pFN21AB9956.seq Check: 7211 from: 1 to: 6757 pFN21AB9956 6757 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4567 278 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4100 1937 Size: 2163 4594 Acc65I G'GTAC_C Cuts at: 4078 4078 Size: 6757 AclI AA'CG_TT Cuts at: 1563 4884 5257 1563 Size: 3321 373 3063 Fragments arranged by size: 3321 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2400 2583 3042 3056 3653 3741 4820 6204 Size: 768 183 459 14 597 88 1079 1384 Cuts at: 6204 6472 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1079 768 597 459 268 183 88 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6560 AflIII A'CryG_T Cuts at: 1203 3213 5656 6633 1203 Size: 2010 2443 977 1327 Fragments arranged by size: 2443 2010 1327 977 AgeI A'CCGG_T Cuts at: 5588 5588 Size: 6757 AhdI GACnn_n'nnGTC Cuts at: 5486 5486 Size: 6757 AleI CACnn'nnGTG Cuts at: 1166 3333 1166 Size: 2167 4590 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6757 AlwNI CAG_nnn'CTG Cuts at: 4500 6072 4500 Size: 1572 5185 ApaLI G'TGCA_C Cuts at: 4814 5970 4814 Size: 1156 5601 ApoI r'AATT_y Cuts at: 1493 2023 2518 2534 3733 3788 3862 4026 4066 Size: 530 495 16 1199 55 74 164 40 Cuts at: 4066 4292 4444 6553 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1199 530 495 226 164 152 74 55 40 16 AseI AT'TA_AT Cuts at: 160 1032 5311 160 Size: 872 4279 1606 Fragments arranged by size: 4279 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 AvaI C'yCGr_G Cuts at: 1870 1943 4082 1870 Size: 73 2139 4545 Fragments arranged by size: 4545 2139 73 AvrII C'CTAG_G Cuts at: 2809 2809 Size: 6757 BamHI G'GATC_C Cuts at: 4087 4454 4087 Size: 367 6390 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2140 4078 5533 618 Size: 325 217 627 353 1938 1455 1842 Fragments arranged by size: 1938 1842 1455 627 353 325 217 BanII G_rGCy'C Cuts at: 1391 2045 2303 3279 4076 1391 Size: 654 258 976 797 4072 Fragments arranged by size: 4072 976 797 654 258 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5786 BbvCI CC'TCA_GC Cuts at: 3933 3933 Size: 6757 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4948 4982 1903 Size: 34 3011 34 3678 Fragments arranged by size: 3678 3011 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3150 3271 4649 5859 3150 Size: 121 1378 1210 4048 Fragments arranged by size: 4048 1378 1210 121 BclI T'GATC_A Cuts at: 1548 1548 Size: 6757 BfrBI ATG'CAT Cuts at: 2920 3437 2920 Size: 517 6240 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2958 5368 136 Size: 107 122 71 631 605 1286 2410 1525 Fragments arranged by size: 2410 1525 1286 631 605 122 107 71 BglII A'GATC_T Cuts at: 6752 6752 Size: 6757 BlpI GC'TnA_GC Cuts at: 4174 4174 Size: 6757 Bme1580I G_kGCm'C Cuts at: 1790 4818 5974 1790 Size: 3028 1156 2573 Fragments arranged by size: 3028 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6757 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2250 2636 2758 Size: 416 53 83 399 24 833 386 122 Cuts at: 2758 2833 2965 3259 4488 5446 442 Size: 75 132 294 1229 958 1753 Fragments arranged by size: 1753 1229 958 833 416 399 386 294 132 122 83 75 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6757 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2000 2528 2900 5417 1239 Size: 761 528 372 2517 2579 Fragments arranged by size: 2579 2517 761 528 372 Bpu10I CC'TnA_GC Cuts at: 3933 3933 Size: 6757 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3398 4882 5747 6045 3398 Size: 1484 865 298 4110 Fragments arranged by size: 4110 1484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5420 882 Size: 617 3921 2219 Fragments arranged by size: 3921 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4548 493 Size: 711 3344 2702 Fragments arranged by size: 3344 2702 711 BsaBI GATnn'nnATC Cuts at: 6751 6751 Size: 6757 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4564 4946 5581 275 Size: 53 83 186 936 3031 382 635 1451 Fragments arranged by size: 3031 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5190 5588 5862 6009 6420 5190 Size: 398 274 147 411 5527 Fragments arranged by size: 5527 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2193 2223 1178 Size: 30 165 30 790 30 5712 Fragments arranged by size: 5712 790 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2499 1186 Size: 1313 5444 BseYI C'CCAG_C Cuts at: 2086 2305 3197 5960 6456 2086 Size: 219 892 2763 496 2387 Fragments arranged by size: 2763 2387 892 496 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6184 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4207 4968 5996 1540 Size: 275 186 2206 761 1028 2301 Fragments arranged by size: 2301 2206 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2303 3279 4818 4903 5974 2303 Size: 976 1539 85 1071 3086 Fragments arranged by size: 3086 1539 1071 976 85 BsmI GAATG_Cn' Cuts at: 2335 2810 2920 4268 4361 2335 Size: 475 110 1348 93 4731 Fragments arranged by size: 4731 1348 475 110 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2303 3279 4076 4818 4903 5974 Size: 399 255 258 976 797 742 85 1071 Cuts at: 5974 1391 Size: 2174 Fragments arranged by size: 2174 1071 976 797 742 399 258 255 85 BspEI T'CCGG_A Cuts at: 6420 6420 Size: 6757 BspHI T'CATG_A Cuts at: 4646 6376 4646 Size: 1730 5027 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4112 844 Size: 3268 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4644 1394 Size: 429 2821 3507 Fragments arranged by size: 3507 2821 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2402 2980 5252 5426 65 Size: 1183 1154 578 2272 174 1396 Fragments arranged by size: 2272 1396 1183 1154 578 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5401 5588 1700 Size: 110 3591 187 2869 Fragments arranged by size: 3591 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2574 96 Size: 2478 4279 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6757 BssSI C'ACGA_G Cuts at: 4817 5829 4817 Size: 1012 5745 BstAPI GCAn_nnn'nTGC Cuts at: 794 2327 794 Size: 1533 5224 BstBI TT'CG_AA Cuts at: 5561 5561 Size: 6757 BstEII G'GTnAC_C Cuts at: 1184 3161 1184 Size: 1977 4780 BstYI r'GATC_y Cuts at: 1972 4087 4454 4839 4856 6308 6394 6406 6752 Size: 2115 367 385 17 1452 86 12 346 Cuts at: 6752 1972 Size: 1977 Fragments arranged by size: 2115 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2385 2385 Size: 6757 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5266 Fragments arranged by size: 5266 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6018 Fragments arranged by size: 6018 683 56 BtsI GCAGTG_nn' Cuts at: 2788 4273 5067 5087 2788 Size: 1485 794 20 4458 Fragments arranged by size: 4458 1485 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6757 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6722 DraI TTT'AAA Cuts at: 2250 3402 3621 4061 2250 Size: 1152 219 440 4946 Fragments arranged by size: 4946 1152 440 219 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5764 809 Size: 507 4448 1802 Fragments arranged by size: 4448 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2708 4204 5093 Size: 54 1425 50 275 18 878 1496 889 Cuts at: 5093 6722 8 Size: 1629 43 Fragments arranged by size: 1629 1496 1425 889 878 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4204 1537 Size: 275 2392 4090 Fragments arranged by size: 4090 2392 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 4687 1353 Size: 1026 2308 3423 Fragments arranged by size: 3423 2308 1026 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5344 5718 5864 387 Size: 1064 138 3755 374 146 1280 Fragments arranged by size: 3755 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2301 3277 2301 Size: 976 5781 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2000 2400 2528 2583 2900 3042 3056 Size: 393 368 400 128 55 317 142 14 Cuts at: 3056 3653 3741 4820 5417 6204 6472 1239 Size: 597 88 1079 597 787 268 1524 Fragments arranged by size: 1524 1079 787 597 597 400 393 368 317 268 142 128 88 55 14 EcoRI G'AATT_C Cuts at: 4066 4444 4066 Size: 378 6379 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2179 2613 2179 Size: 434 6323 FspI TGC'GCA Cuts at: 5263 5263 Size: 6757 HaeII r_GCGC'y Cuts at: 1678 5904 1678 Size: 4226 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2908 2940 3098 3130 3737 Size: 32 135 32 1336 32 158 32 607 Cuts at: 3737 3769 5398 5430 5472 5504 1373 Size: 32 1629 32 42 32 2626 Fragments arranged by size: 2626 1629 1336 607 158 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4101 4347 669 Size: 988 281 2163 246 3079 Fragments arranged by size: 3079 2163 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6757 HpaI GTT'AAC Cuts at: 4347 4347 Size: 6757 KpnI G_GTAC'C Cuts at: 4082 4082 Size: 6757 MfeI C'AATT_G Cuts at: 4356 4356 Size: 6757 MluI A'CGCG_T Cuts at: 6633 6633 Size: 6757 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2430 2461 5871 6055 1305 Size: 1125 31 3410 184 2007 Fragments arranged by size: 3410 2007 1125 184 31 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4935 Fragments arranged by size: 4935 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2118 2166 3333 4715 5074 5233 518 Size: 648 952 48 1167 1382 359 159 2042 Fragments arranged by size: 2042 1382 1167 952 648 359 159 48 MspA1I CmG'CkG Cuts at: 1606 4173 4850 5998 6243 1606 Size: 2567 677 1148 245 2120 Fragments arranged by size: 2567 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5266 Fragments arranged by size: 5266 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6757 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6757 NotI GC'GGCC_GC Cuts at: 4204 4204 Size: 6757 NsiI A_TGCA'T Cuts at: 2922 3439 2922 Size: 517 6240 NspI r_CATG'y Cuts at: 1132 1229 2210 2353 3217 3773 4115 5660 1132 Size: 97 981 143 864 556 342 1545 2229 Fragments arranged by size: 2229 1545 981 864 556 342 143 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6757 PciI A'CATG_T Cuts at: 3213 5656 3213 Size: 2443 4314 PflMI CCAn_nnn'nTGG Cuts at: 1108 2657 1108 Size: 1549 5208 PmeI GTTT'AAAC Cuts at: 4061 4061 Size: 6757 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2707 2739 4826 4858 6339 6371 2707 Size: 32 2087 32 1481 32 3093 Fragments arranged by size: 3093 2087 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6757 PsiI TTA'TAA Cuts at: 3149 4327 3149 Size: 1178 5579 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2990 3022 2990 Size: 32 6725 PstI C_TGCA'G Cuts at: 830 4109 830 Size: 3279 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6757 SacI G_AGCT'C Cuts at: 2303 3279 2303 Size: 976 5781 SalI G'TCGA_C Cuts at: 1936 4099 1936 Size: 2163 4594 SbfI CC_TGCA'GG Cuts at: 4109 4109 Size: 6757 ScaI AGT'ACT Cuts at: 5005 6717 5005 Size: 1712 5045 SfcI C'TryA_G Cuts at: 826 1045 4105 5240 5921 6112 826 Size: 219 3060 1135 681 191 1471 Fragments arranged by size: 3060 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6757 SmaI CCC'GGG Cuts at: 1872 4084 1872 Size: 2212 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3377 4861 5762 6024 820 Size: 197 926 1434 1484 901 262 1553 Fragments arranged by size: 1553 1484 1434 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4548 493 Size: 4055 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6757 SphI G_CATG'C Cuts at: 1132 4115 1132 Size: 2983 3774 SspI AAT'ATT Cuts at: 5 52 4681 5 Size: 47 4629 2081 Fragments arranged by size: 4629 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6757 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2809 4196 513 Size: 552 939 27 778 1387 3074 Fragments arranged by size: 3074 1387 939 778 552 27 SwaI ATTT'AAAT Cuts at: 2250 2250 Size: 6757 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 4784 4801 4954 5139 5584 325 Size: 1255 717 2487 17 153 185 445 1498 Fragments arranged by size: 2487 1498 1255 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2222 2574 2594 Size: 274 80 33 51 1443 245 352 20 Cuts at: 2594 5003 6715 96 Size: 2409 1712 138 Fragments arranged by size: 2409 1712 1443 352 274 245 138 80 51 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6717 6749 6717 Size: 32 6725 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6757 XbaI T'CTAG_A Cuts at: 4093 4093 Size: 6757 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6757 XmaI C'CCGG_G Cuts at: 1870 4082 1870 Size: 2212 4545 XmnI GAAnn'nnTTC Cuts at: 4886 4886 Size: 6757 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4565 276 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI