Restriction map for
pFN21AE5787
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AccIII AflII AflIII AgeI
AhdI Alw44I AocI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BlpI Bpu1102I BsaAI
BsaBI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI BstEII BstPI Bsu36I
CelII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NarI NciI NdeI NgoMIV NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 2188 GACGTC GACGT^C
AccI 1444 GTATAC GT^[AC][GT]AC
AclI 496 AACGTT AA^CGTT
AlwNI 1516 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 488 AGCGCT AGC^GCT
AseI 2194 ATTAAT AT^TAAT
AsnI 2194 ATTAAT AT^TAAT
AvrII 60 CCTAGG C^CTAGG
BglI 22 GCCGTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 645 AGATCT A^GATCT
BlnI 60 CCTAGG C^CTAGG
BsaI 1579 GAGACC GGTCTC(1/5)
BsaWI 1820 TCCGGT [AT]^CCGG[AT]
BsrDI 1360 GCAATG GCAATG(2/0)
BsrFI 746 ACCGGC [GA]^CCGG[CT]
Bst1107I 1444 GTATAC GTA^TAC
BstAPI 1740 GCAGTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 848 TTCGAA TT^CGAA
BstXI 611 CCATGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1444 GTATAC GTA^TAC
Cfr10I 746 ACCGGC [GA]^CCGG[CT]
DraIII 324 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 734 CGGCCA [CT]^GGCC[GA]
EarI 851 GAAGAG CTCTTC(1/4)
Eco47III 488 AGCGCT AGC^GCT
FauI 52 CCCGC CCCGC(4/6)
HgaI 2009 GACGC GACGC(5/10)
HincII 2219 GTCAAC GT[CT]^[GA]AC
HindII 2219 GTCAAC GT[CT]^[GA]AC
Ksp632I 851 GAAGAG CTCTTC(1/4)
NheI 840 GCTAGC G^CTAGC
NspV 848 TTCGAA TT^CGAA
PshAI 682 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2194 ATTAAT AT^TAAT
Psp1406I 496 AACGTT AA^CGTT
PvuII 716 CAGCTG CAG^CTG
SapI 851 GAAGAGC GCTCTTC(1/4)
SfuI 848 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 27 CGCG CG^CG
40 CGCG
AcyI 2009 GACGCC G[GA]^CG[CT]C
2188 GACGTC
AfaI 451 GTAC GT^AC
1177 GTAC
AspHI 1841 GAGCAC G[AT]GC[AT]^C
1895 GTGCTC
AvaII 528 GGACC G^G[AT]CC
1677 GGACC
BbsI 2185 GAAGAC GAAGAC(2/6)
105 GTCTTC
BpuAI 2185 GAAGAC GAAGAC(2/6)
105 GTCTTC
BsaHI 2009 GACGCC G[GA]^CG[CT]C
2188 GACGTC
BsgI 419 GTGCAG GTGCAG(16/14)
1634 GTGCAG
BsiHKAI 1841 GAGCAC G[AT]GC[AT]^C
1895 GTGCTC
BstUI 27 CGCG CG^CG
40 CGCG
BstYI 645 AGATCT [GA]^GATC[CT]
1428 AGATCC
EcoT22I 226 ATGCAT ATGCA^T
1201 ATGCAT
HaeII 488 AGCGCT [GA]GCGC^[CT]
895 AGCGCC
Hin1I 2009 GACGCC G[GA]^CG[CT]C
2188 GACGTC
MaeII 497 ACGT A^CGT
2189 ACGT
MaeIII 1142 GTGAC ^GT[ACGT]AC
1802 GTCAC
MflI 645 AGATCT [GA]^GATC[CT]
1428 AGATCC
MspA1I 716 CAGCTG C[AC]G^C[GT]G
1308 CAGCGG
MvnI 27 CGCG CG^CG
40 CGCG
NsiI 226 ATGCAT ATGCA^T
1201 ATGCAT
PstI 17 CTGCAG CTGCA^G
559 CTGCAG
RsaI 451 GTAC GT^AC
1177 GTAC
SphI 1199 GCATGC GCATG^C
1203 GCATGC
StuI 92 AGGCCT AGG^CCT
1062 AGGCCT
Tsp45I 1142 GTGAC ^GT[GC]AC
1802 GTCAC
XhoII 645 AGATCT [GA]^GATC[CT]
1428 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AluI AlwI ApaI
ApoI BanII BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MboI
MboII MnlI MseI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PleI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I