(Circular) (Six-base) MAPSORT of: pFN21AE5787.seq Check: 3771 from: 1 to: 6958 pFN21AE5787 6958 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4197 4768 278 Size: 53 83 186 936 2661 571 2468 Fragments arranged by size: 2661 2468 936 571 186 83 53 AccI GT'mk_AC Cuts at: 1937 3450 4301 1937 Size: 1513 851 4594 Fragments arranged by size: 4594 1513 851 Acc65I G'GTAC_C Cuts at: 4279 4279 Size: 6958 AclI AA'CG_TT Cuts at: 1563 2502 4257 5085 5458 1563 Size: 939 1755 828 373 3063 Fragments arranged by size: 3063 1755 939 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2988 5021 6405 6673 1632 Size: 1356 2033 1384 268 1917 Fragments arranged by size: 2033 1917 1384 1356 268 AfeI AGC'GCT Cuts at: 2495 2495 Size: 6958 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6761 AflIII A'CryG_T Cuts at: 1203 5857 6834 1203 Size: 4654 977 1327 Fragments arranged by size: 4654 1327 977 AgeI A'CCGG_T Cuts at: 5789 5789 Size: 6958 AhdI GACnn_n'nnGTC Cuts at: 5687 5687 Size: 6958 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6958 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6958 AlwNI CAG_nnn'CTG Cuts at: 3526 4701 6273 3526 Size: 1175 1572 4211 Fragments arranged by size: 4211 1572 1175 ApaI G_GGCC'C Cuts at: 2056 2407 2563 3797 2056 Size: 351 156 1234 5217 Fragments arranged by size: 5217 1234 351 156 ApaLI G'TGCA_C Cuts at: 5015 6171 5015 Size: 1156 5802 ApoI r'AATT_y Cuts at: 1493 3398 3707 3980 4267 4493 4645 6754 1493 Size: 1905 309 273 287 226 152 2109 1697 Fragments arranged by size: 2109 1905 1697 309 287 273 226 152 AseI AT'TA_AT Cuts at: 160 1032 4200 5512 160 Size: 872 3168 1312 1606 Fragments arranged by size: 3168 1606 1312 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6958 AvaI C'yCGr_G Cuts at: 1870 1943 4283 1870 Size: 73 2340 4545 Fragments arranged by size: 4545 2340 73 AvrII C'CTAG_G Cuts at: 2065 2065 Size: 6958 BamHI G'GATC_C Cuts at: 4288 4655 4288 Size: 367 6591 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4279 5734 618 Size: 325 217 627 2492 1455 1842 Fragments arranged by size: 2492 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2056 2407 2546 2563 3797 3825 4277 1391 Size: 665 351 139 17 1234 28 452 4072 Fragments arranged by size: 4072 1234 665 452 351 139 28 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2103 4197 928 Size: 971 204 2094 3689 Fragments arranged by size: 3689 2094 971 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5149 5183 1903 Size: 34 3212 34 3678 Fragments arranged by size: 3678 3212 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4850 6060 4850 Size: 1210 5748 BclI T'GATC_A Cuts at: 1548 1548 Size: 6958 BfrBI ATG'CAT Cuts at: 2233 3208 2233 Size: 975 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2033 5569 136 Size: 107 122 71 631 605 361 3536 1525 Fragments arranged by size: 3536 1525 631 605 361 122 107 71 BglII A'GATC_T Cuts at: 2650 6953 2650 Size: 4303 2655 BlpI GC'TnA_GC Cuts at: 4375 4375 Size: 6958 Bme1580I G_kGCm'C Cuts at: 1790 2056 2407 2563 3797 5019 6175 1790 Size: 266 351 156 1234 1222 1156 2573 Fragments arranged by size: 2573 1234 1222 1156 351 266 156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6958 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4041 4689 5647 Size: 416 53 83 399 24 2624 648 958 Cuts at: 5647 442 Size: 1753 Fragments arranged by size: 2624 1753 958 648 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2849 1056 Size: 1793 5165 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3022 3022 Size: 6958 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3022 3104 3692 5618 1239 Size: 1783 82 588 1926 2579 Fragments arranged by size: 2579 1926 1783 588 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5083 5948 6246 5083 Size: 865 298 5795 Fragments arranged by size: 5795 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3578 5621 882 Size: 617 2079 2043 2219 Fragments arranged by size: 2219 2079 2043 617 BsaAI yAC'GTr Cuts at: 493 1204 4749 493 Size: 711 3545 2702 Fragments arranged by size: 3545 2702 711 BsaBI GATnn'nnATC Cuts at: 6952 6952 Size: 6958 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4015 4194 4765 5147 Size: 53 83 186 936 2482 179 571 382 Cuts at: 5147 5782 275 Size: 635 1451 Fragments arranged by size: 2482 1451 936 635 571 382 186 179 83 53 BsaWI w'CCGG_w Cuts at: 3825 5391 5789 6063 6210 6621 3825 Size: 1566 398 274 147 411 4162 Fragments arranged by size: 4162 1566 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6733 Fragments arranged by size: 6733 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6958 BseYI C'CCAG_C Cuts at: 2551 6161 6657 2551 Size: 3610 496 2852 Fragments arranged by size: 3610 2852 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2445 3660 1163 Size: 573 709 1215 4461 Fragments arranged by size: 4461 1215 709 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4408 5169 6197 1540 Size: 275 186 2407 761 1028 2301 Fragments arranged by size: 2407 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3850 3904 5019 5104 6175 3850 Size: 54 1115 85 1071 4633 Fragments arranged by size: 4633 1115 1071 85 54 BsmI GAATG_Cn' Cuts at: 4469 4562 4469 Size: 93 6865 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2407 2546 2563 3797 3825 3850 Size: 399 266 351 139 17 1234 28 25 Cuts at: 3850 3904 4277 5019 5104 6175 1391 Size: 54 373 742 85 1071 2174 Fragments arranged by size: 2174 1234 1071 742 399 373 351 266 139 85 54 28 25 17 BspEI T'CCGG_A Cuts at: 6621 6621 Size: 6958 BspHI T'CATG_A Cuts at: 4847 6577 4847 Size: 1730 5228 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2074 3408 4149 4313 844 Size: 1230 1334 741 164 3489 Fragments arranged by size: 3489 1334 1230 741 164 BsrBI CCG'CTC Cuts at: 1394 1823 4845 1394 Size: 429 3022 3507 Fragments arranged by size: 3507 3022 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3372 5453 5627 65 Size: 1183 2124 2081 174 1396 Fragments arranged by size: 2124 2081 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2751 5602 5789 1700 Size: 110 941 2851 187 2869 Fragments arranged by size: 2869 2851 941 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6958 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6958 BssSI C'ACGA_G Cuts at: 5018 6030 5018 Size: 1012 5946 BstAPI GCAn_nnn'nTGC Cuts at: 794 3751 794 Size: 2957 4001 BstBI TT'CG_AA Cuts at: 2854 5762 2854 Size: 2908 4050 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6958 BstXI CCAn_nnnn'nTGG Cuts at: 2623 2623 Size: 6958 BstYI r'GATC_y Cuts at: 1972 2650 3433 4288 4655 5040 5057 6509 6595 Size: 678 783 855 367 385 17 1452 86 Cuts at: 6595 6607 6953 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 855 783 678 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3451 3451 Size: 6958 BtgI C'CryG_G Cuts at: 513 1065 2277 2616 4018 513 Size: 552 1212 339 1402 3453 Fragments arranged by size: 3453 1402 1212 552 339 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6219 Fragments arranged by size: 6219 683 56 BtsI GCAGTG_nn' Cuts at: 4474 5268 5288 4474 Size: 794 20 6144 Fragments arranged by size: 6144 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6958 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2376 2411 529 Size: 35 1812 35 5076 Fragments arranged by size: 5076 1812 35 35 DraI TTT'AAA Cuts at: 2638 2841 3079 3445 4262 2638 Size: 203 238 366 817 5334 Fragments arranged by size: 5334 817 366 238 203 DraIII CAC_nnn'GTG Cuts at: 2334 2334 Size: 6958 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5965 809 Size: 507 4649 1802 Fragments arranged by size: 4649 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2739 4405 5294 Size: 54 1425 50 275 18 909 1666 889 Cuts at: 5294 6923 8 Size: 1629 43 Fragments arranged by size: 1666 1629 1425 909 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4405 1537 Size: 275 2593 4090 Fragments arranged by size: 4090 2593 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2851 4888 1353 Size: 1498 2037 3423 Fragments arranged by size: 3423 2037 1498 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5545 5919 6065 387 Size: 1064 138 3956 374 146 1280 Fragments arranged by size: 3956 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2988 3022 3104 3692 5021 5618 6405 Size: 393 1356 34 82 588 1329 597 787 Cuts at: 6405 6673 1239 Size: 268 1524 Fragments arranged by size: 1524 1356 1329 787 597 588 393 268 82 34 EcoO109I rG'GnC_Cy Cuts at: 2052 2053 2403 2404 2559 2560 3793 3794 2052 Size: 1 350 1 155 1 1233 1 5216 Fragments arranged by size: 5216 1233 350 155 1 1 1 1 EcoRI G'AATT_C Cuts at: 4267 4645 4267 Size: 378 6580 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3920 3920 Size: 6958 FspI TGC'GCA Cuts at: 5464 5464 Size: 6958 HaeII r_GCGC'y Cuts at: 1678 2497 2904 6105 1678 Size: 819 407 3201 2531 Fragments arranged by size: 3201 2531 819 407 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2464 2496 5599 5631 5673 Size: 32 135 32 892 32 3103 32 42 Cuts at: 5673 5705 1373 Size: 32 2626 Fragments arranged by size: 3103 2626 892 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4226 4302 4548 669 Size: 988 281 2288 76 246 3079 Fragments arranged by size: 3079 2288 988 281 246 76 HindIII A'AGCT_T Cuts at: 748 748 Size: 6958 HpaI GTT'AAC Cuts at: 4548 4548 Size: 6958 KpnI G_GTAC'C Cuts at: 4283 4283 Size: 6958 MfeI C'AATT_G Cuts at: 4557 4557 Size: 6958 MluI A'CGCG_T Cuts at: 6834 6834 Size: 6958 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2397 2428 2571 6072 6256 1305 Size: 1092 31 143 3501 184 2007 Fragments arranged by size: 3501 2007 1092 184 143 31 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5136 Fragments arranged by size: 5136 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4916 5275 5434 518 Size: 648 3750 359 159 2042 Fragments arranged by size: 3750 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2723 3315 4374 5051 6199 6444 1606 Size: 1117 592 1059 677 1148 245 2120 Fragments arranged by size: 2120 1148 1117 1059 677 592 245 NcoI C'CATG_G Cuts at: 513 1065 2277 2616 4018 513 Size: 552 1212 339 1402 3453 Fragments arranged by size: 3453 1402 1212 552 339 NdeI CA'TA_TG Cuts at: 387 387 Size: 6958 NheI G'CTAG_C Cuts at: 1052 2845 1052 Size: 1793 5165 NotI GC'GGCC_GC Cuts at: 4405 4405 Size: 6958 NsiI A_TGCA'T Cuts at: 2235 3210 2235 Size: 975 5983 NspI r_CATG'y Cuts at: 1132 1229 2378 3208 3212 4316 5861 1132 Size: 97 1149 830 4 1104 1545 2229 Fragments arranged by size: 2229 1545 1149 1104 830 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6958 PciI A'CATG_T Cuts at: 5857 5857 Size: 6958 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6958 PfoI T'CCnGG_A Cuts at: 3669 3669 Size: 6958 PmeI GTTT'AAAC Cuts at: 4262 4262 Size: 6958 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5027 5059 6540 6572 5027 Size: 32 1481 32 5413 Fragments arranged by size: 5413 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2691 1613 Size: 1078 5880 PsiI TTA'TAA Cuts at: 4528 4528 Size: 6958 PspOMI G'GGCC_C Cuts at: 2052 2403 2559 3793 2052 Size: 351 156 1234 5217 Fragments arranged by size: 5217 1234 351 156 PstI C_TGCA'G Cuts at: 830 2026 2568 4310 830 Size: 1196 542 1742 3478 Fragments arranged by size: 3478 1742 1196 542 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6958 PvuII CAG'CTG Cuts at: 2723 2723 Size: 6958 SalI G'TCGA_C Cuts at: 1936 4300 1936 Size: 2364 4594 SapI GCTCTTCn'nnn_ Cuts at: 2851 2851 Size: 6958 SbfI CC_TGCA'GG Cuts at: 4310 4310 Size: 6958 ScaI AGT'ACT Cuts at: 5206 6918 5206 Size: 1712 5246 SfcI C'TryA_G Cuts at: 826 1045 2022 2564 3759 4218 4306 5441 6122 Size: 219 977 542 1195 459 88 1135 681 Cuts at: 6122 6313 826 Size: 191 1471 Fragments arranged by size: 1471 1195 1135 977 681 542 459 219 191 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6958 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6958 SmaI CCC'GGG Cuts at: 1872 4285 1872 Size: 2413 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5062 5963 6225 820 Size: 197 926 3119 901 262 1553 Fragments arranged by size: 3119 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4749 493 Size: 4256 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6958 SphI G_CATG'C Cuts at: 1132 3208 3212 4316 1132 Size: 2076 4 1104 3774 Fragments arranged by size: 3774 2076 1104 4 SspI AAT'ATT Cuts at: 5 52 2149 2350 3736 4882 5 Size: 47 2097 201 1386 1146 2081 Fragments arranged by size: 2097 2081 1386 1146 201 47 StuI AGG'CCT Cuts at: 1517 2099 3069 1517 Size: 582 970 5406 Fragments arranged by size: 5406 970 582 StyI C'CwwG_G Cuts at: 513 1065 2065 2277 2616 3915 4018 4397 513 Size: 552 1000 212 339 1299 103 379 3074 Fragments arranged by size: 3074 1299 1000 552 379 339 212 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2550 3072 4985 5002 5155 5340 5785 Size: 1255 970 522 1913 17 153 185 445 Cuts at: 5785 325 Size: 1498 Fragments arranged by size: 1913 1498 1255 970 522 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5204 6916 96 Size: 274 80 33 51 1443 3227 1712 138 Fragments arranged by size: 3227 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6918 6950 6918 Size: 32 6926 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6958 XbaI T'CTAG_A Cuts at: 4294 4294 Size: 6958 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6958 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6958 XmaI C'CCGG_G Cuts at: 1870 4283 1870 Size: 2413 4545 XmnI GAAnn'nnTTC Cuts at: 5087 5087 Size: 6958 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4195 4766 276 Size: 53 83 186 936 2661 571 2468 Fragments arranged by size: 2661 2468 936 571 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI