(Linear) (Six-base) MAPSORT of: KIEE5787.seq Check: 1187 from: 1 to: 2253 KIEE5787.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. AatII G_ACGT'C Cuts at: 0 2192 2253 Size: 2192 61 AccI GT'mk_AC Cuts at: 0 1445 2253 Size: 1445 808 AclI AA'CG_TT Cuts at: 0 497 2253 Size: 497 1756 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 983 2253 Size: 983 1270 AfeI AGC'GCT Cuts at: 0 490 2253 Size: 490 1763 AlwNI CAG_nnn'CTG Cuts at: 0 1521 2253 Size: 1521 732 ApaI G_GGCC'C Cuts at: 0 51 402 558 1792 2253 Size: 51 351 156 1234 461 Fragments arranged by size: 1234 461 351 156 51 ApoI r'AATT_y Cuts at: 0 1393 1702 1975 2253 Size: 1393 309 273 278 Fragments arranged by size: 1393 309 278 273 AseI AT'TA_AT Cuts at: 0 2195 2253 Size: 2195 58 AvrII C'CTAG_G Cuts at: 0 60 2253 Size: 60 2193 BanII G_rGCy'C Cuts at: 0 51 402 541 558 1792 1820 2253 Size: 51 351 139 17 1234 28 433 Fragments arranged by size: 1234 433 351 139 51 28 17 BbsI GAAGACnn'nnnn_ Cuts at: 0 98 2192 2253 Size: 98 2094 61 Fragments arranged by size: 2094 98 61 BfrBI ATG'CAT Cuts at: 0 228 1203 2253 Size: 228 975 1050 Fragments arranged by size: 1050 975 228 BglI GCCn_nnn'nGGC Cuts at: 0 28 2253 Size: 28 2225 BglII A'GATC_T Cuts at: 0 645 2253 Size: 645 1608 Bme1580I G_kGCm'C Cuts at: 0 51 402 558 1792 2253 Size: 51 351 156 1234 461 Fragments arranged by size: 1234 461 351 156 51 BmrI ACTGGGnnnn_n' Cuts at: 0 2036 2253 Size: 2036 217 BmtI G_CTAG'C Cuts at: 0 844 2253 Size: 844 1409 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1017 2253 Size: 1017 1236 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1017 1099 1687 2253 Size: 1017 82 588 566 Fragments arranged by size: 1017 588 566 82 BsaI GGTCTCn'nnnn_ Cuts at: 0 1573 2253 Size: 1573 680 BsaHI Gr'CG_yC Cuts at: 0 2010 2189 2253 Size: 2010 179 64 Fragments arranged by size: 2010 179 64 BsaWI w'CCGG_w Cuts at: 0 1820 2253 Size: 1820 433 BseYI C'CCAG_C Cuts at: 0 546 2253 Size: 546 1707 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 440 1655 2253 Size: 440 1215 598 Fragments arranged by size: 1215 598 440 BsiHKAI G_wGCw'C Cuts at: 0 1845 1899 2253 Size: 1845 54 354 Fragments arranged by size: 1845 354 54 Bsp1286I G_dGCh'C Cuts at: 0 51 402 541 558 1792 1820 1845 1899 Size: 51 351 139 17 1234 28 25 54 Cuts at: 1899 2253 Size: 354 Fragments arranged by size: 1234 354 351 139 54 51 28 25 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 69 1403 2144 2253 Size: 69 1334 741 109 Fragments arranged by size: 1334 741 109 69 BsrDI GCAATG_nn' Cuts at: 0 1367 2253 Size: 1367 886 BsrFI r'CCGG_y Cuts at: 0 746 2253 Size: 746 1507 BstAPI GCAn_nnn'nTGC Cuts at: 0 1746 2253 Size: 1746 507 BstBI TT'CG_AA Cuts at: 0 849 2253 Size: 849 1404 BstXI CCAn_nnnn'nTGG Cuts at: 0 618 2253 Size: 618 1635 BstYI r'GATC_y Cuts at: 0 645 1428 2253 Size: 645 783 825 Fragments arranged by size: 825 783 645 BstZ17I GTA'TAC Cuts at: 0 1446 2253 Size: 1446 807 BtgI C'CryG_G Cuts at: 0 272 611 2013 2253 Size: 272 339 1402 240 Fragments arranged by size: 1402 339 272 240 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 371 406 2253 Size: 371 35 1847 Fragments arranged by size: 1847 371 35 DraI TTT'AAA Cuts at: 0 633 836 1074 1440 2253 Size: 633 203 238 366 813 Fragments arranged by size: 813 633 366 238 203 DraIII CAC_nnn'GTG Cuts at: 0 329 2253 Size: 329 1924 EaeI y'GGCC_r Cuts at: 0 734 2253 Size: 734 1519 EarI CTCTTCn'nnn_ Cuts at: 0 846 2253 Size: 846 1407 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 983 1017 1099 1687 2253 Size: 983 34 82 588 566 Fragments arranged by size: 983 588 566 82 34 EcoO109I rG'GnC_Cy Cuts at: 0 47 48 398 399 554 555 1788 1789 Size: 47 1 350 1 155 1 1233 1 Cuts at: 1789 2253 Size: 464 Fragments arranged by size: 1233 464 350 155 47 1 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1915 2253 Size: 1915 338 HaeII r_GCGC'y Cuts at: 0 492 899 2253 Size: 492 407 1354 Fragments arranged by size: 1354 492 407 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 459 491 2253 Size: 459 32 1762 Fragments arranged by size: 1762 459 32 HincII GTy'rAC Cuts at: 0 2221 2253 Size: 2221 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 392 423 566 2253 Size: 392 31 143 1687 Fragments arranged by size: 1687 392 143 31 MspA1I CmG'CkG Cuts at: 0 718 1310 2253 Size: 718 592 943 Fragments arranged by size: 943 718 592 NcoI C'CATG_G Cuts at: 0 272 611 2013 2253 Size: 272 339 1402 240 Fragments arranged by size: 1402 339 272 240 NheI G'CTAG_C Cuts at: 0 840 2253 Size: 840 1413 NsiI A_TGCA'T Cuts at: 0 230 1205 2253 Size: 230 975 1048 Fragments arranged by size: 1048 975 230 NspI r_CATG'y Cuts at: 0 373 1203 1207 2253 Size: 373 830 4 1046 Fragments arranged by size: 1046 830 373 4 PfoI T'CCnGG_A Cuts at: 0 1664 2253 Size: 1664 589 PshAI GACnn'nnGTC Cuts at: 0 686 2253 Size: 686 1567 PspOMI G'GGCC_C Cuts at: 0 47 398 554 1788 2253 Size: 47 351 156 1234 465 Fragments arranged by size: 1234 465 351 156 47 PstI C_TGCA'G Cuts at: 0 21 563 2253 Size: 21 542 1690 Fragments arranged by size: 1690 542 21 PvuII CAG'CTG Cuts at: 0 718 2253 Size: 718 1535 SapI GCTCTTCn'nnn_ Cuts at: 0 846 2253 Size: 846 1407 SfcI C'TryA_G Cuts at: 0 17 559 1754 2213 2253 Size: 17 542 1195 459 40 Fragments arranged by size: 1195 542 459 40 17 SphI G_CATG'C Cuts at: 0 1203 1207 2253 Size: 1203 4 1046 Fragments arranged by size: 1203 1046 4 SspI AAT'ATT Cuts at: 0 144 345 1731 2253 Size: 144 201 1386 522 Fragments arranged by size: 1386 522 201 144 StuI AGG'CCT Cuts at: 0 94 1064 2253 Size: 94 970 1189 Fragments arranged by size: 1189 970 94 StyI C'CwwG_G Cuts at: 0 60 272 611 1910 2013 2253 Size: 60 212 339 1299 103 240 Fragments arranged by size: 1299 339 240 212 103 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 545 1067 2253 Size: 545 522 1186 Fragments arranged by size: 1186 545 522 ZraI GAC'GTC Cuts at: 0 2190 2253 Size: 2190 63 Enzymes that do cut: AatII AccI AclI AcuI AfeI AlwNI ApaI ApoI AseI AvrII BanII BbsI BfrBI BglI BglII Bme1580I BmrI BmtI BplI BpmI BsaI BsaHI BsaWI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstAPI BstBI BstXI BstYI BstZ17I BtgI CspCI DraI DraIII EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII MmeI MspA1I NcoI NheI NsiI NspI PfoI PshAI PspOMI PstI PvuII SapI SfcI SphI SspI StuI StyI TaqII ZraI Enzymes that do not cut: AarI Acc65I AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AsiSI AvaI BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BlpI BmgBI Bpu10I BpuEI BsaAI BsaBI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstEII Bsu36I BtgZI BtsI ClaI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI