Restriction map for
pFN21AB7912
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanII BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiHKAI BsiWI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII Eam1105I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MunI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TspRI Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 72 GTAC GT^AC
AlwNI 523 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 763 AGCGCT AGC^GCT
AvaI 545 CCCGAG C^[CT]CG[GA]G
AvaII 431 GGACC G^G[AT]CC
BanI 298 GGCGCC G^G[CT][GA]CC
BbeI 298 GGCGCC GGCGC^C
BglI 859 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 69 CACGTA [CT]AC^GT[GA]
BsaI 481 GAGACC GGTCTC(1/5)
BsmBI 21 CGTCTC CGTCTC(1/5)
BsmFI 348 GTCCC GGGAC(10/14)
BsoBI 545 CCCGAG C^[CT]CG[GA]G
BsrDI 709 CATTGC GCAATG(2/0)
DsaI 869 CCGCGG C^C[GA][CT]GG
EarI 572 GAAGAG CTCTTC(1/4)
Eco47III 763 AGCGCT AGC^GCT
EcoNI 534 CCTGTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 110 CCAAGG C^C[AT][AT]GG
HincII 667 GTCAAC GT[CT]^[GA]AC
HindII 667 GTCAAC GT[CT]^[GA]AC
HindIII 644 AAGCTT A^AGCTT
HphI 440 TCACC GGTGA(8/7)
KasI 298 GGCGCC G^GCGCC
Ksp632I 572 GAAGAG CTCTTC(1/4)
KspI 869 CCGCGG CCGC^GG
MaeIII 718 GTGAC ^GT[ACGT]AC
MseI 682 TTAA T^TAA
MslI 607 CATAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 175 GCCGGC GCC^GGC
NarI 298 GGCGCC GG^CGCC
NgoMIV 175 GCCGGC G^CCGGC
NotI 901 GCGGCCGC GC^GGCCGC
PflMI 522 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 653 GAGTC GAGTC(4/5)
PstI 751 CTGCAG CTGCA^G
RsaI 72 GTAC GT^AC
SacII 869 CCGCGG CCGC^GG
SapI 572 GAAGAGC GCTCTTC(1/4)
SfcI 751 CTGCAG C^T[GA][CT]AG
SfoI 298 GGCGCC GGC^GCC
StyI 110 CCAAGG C^C[AT][AT]GG
TaqI 570 TCGA T^CGA
TfiI 874 GATTC G^A[AT]TC
Tru9I 682 TTAA T^TAA
Tsp45I 718 GTGAC ^GT[GC]AC
Tsp509I 436 AATT ^AATT
TthHB8I 570 TCGA T^CGA
Van91I 522 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 434 CCAATTTCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BsgI 126 CTGCAC GTGCAG(16/14)
550 GTGCAG
BsmAI 481 GAGAC GTCTC(1/5)
22 GTCTC
BspMI 788 ACCTGC ACCTGC(4/8)
552 GCAGGT
BsrBI 853 CCGCTC CCGCTC(-3/-3)
90 GAGCGG
BsrFI 121 ACCGGC [GA]^CCGG[CT]
175 GCCGGC
BsrI 528 ACTGG ACTGG(1/-1)
636 CCAGT
Cfr10I 121 ACCGGC [GA]^CCGG[CT]
175 GCCGGC
DdeI 517 CTGAG C^T[ACGT]AG
952 CTCAG
DrdI 339 GACGGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
720 GACGCCCTTGTC
EagI 173 CGGCCG C^GGCCG
902 CGGCCG
EclXI 173 CGGCCG C^GGCCG
902 CGGCCG
Eco52I 173 CGGCCG C^GGCCG
902 CGGCCG
HgaI 496 GACGC GACGC(5/10)
720 GACGC
HinfI 653 GAGTC G^A[ACGT]TC
874 GATTC
PvuII 748 CAGCTG CAG^CTG
754 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AluI BbvI
BcnI BpmI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI