(Circular) (Six-base) MAPSORT of: pFN21AB7912.seq Check: 1474 from: 1 to: 5698 pFN21AB7912 5698 bp With 182 enzymes: SgfI * March 28, 2012 12:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2802 2802 Size: 5698 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3041 1937 Size: 1104 4594 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3761 5145 5413 1632 Size: 2129 1384 268 1917 Fragments arranged by size: 2129 1917 1384 268 AfeI AGC'GCT Cuts at: 2770 2770 Size: 5698 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 4597 5574 1203 Size: 3394 977 1327 Fragments arranged by size: 3394 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 2533 3441 5013 2533 Size: 908 1572 3218 Fragments arranged by size: 3218 1572 908 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 2550 3023 1870 Size: 73 607 473 4545 Fragments arranged by size: 4545 607 473 73 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 3019 4474 618 Size: 325 217 627 516 716 1455 1842 Fragments arranged by size: 1842 1455 716 627 516 325 217 BanII G_rGCy'C Cuts at: 1391 3017 1391 Size: 1626 4072 BbeI G_GCGC'C Cuts at: 2307 2307 Size: 5698 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4727 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2870 4309 136 Size: 107 122 71 631 605 1198 1439 1525 Fragments arranged by size: 1525 1439 1198 631 605 122 107 71 BglII A'GATC_T Cuts at: 5693 5693 Size: 5698 BlpI GC'TnA_GC Cuts at: 3115 3115 Size: 5698 Bme1580I G_kGCm'C Cuts at: 1790 3759 4915 1790 Size: 1969 1156 2573 Fragments arranged by size: 2573 1969 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2543 3429 4387 Size: 416 53 83 399 24 1126 886 958 Cuts at: 4387 442 Size: 1753 Fragments arranged by size: 1753 1126 958 886 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2286 2489 2926 4358 1239 Size: 872 175 203 437 1432 2579 Fragments arranged by size: 2579 1432 872 437 203 175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3823 4688 4986 3823 Size: 865 298 4535 Fragments arranged by size: 4535 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2480 4361 882 Size: 617 981 1881 2219 Fragments arranged by size: 2219 1881 981 617 BsaAI yAC'GTr Cuts at: 493 1204 2076 3489 493 Size: 711 872 1413 2702 Fragments arranged by size: 2702 1413 872 711 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 2502 2726 3505 Size: 53 83 186 936 771 198 224 779 Cuts at: 3505 3887 4522 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 779 771 635 382 224 198 186 83 53 BsaWI w'CCGG_w Cuts at: 4131 4529 4803 4950 5361 4131 Size: 398 274 147 411 4468 Fragments arranged by size: 4468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2220 2250 1178 Size: 30 165 30 817 30 4626 Fragments arranged by size: 4626 817 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2214 2235 2244 2256 2259 2262 2274 2283 Size: 1028 21 9 12 3 3 12 9 Cuts at: 2283 1186 Size: 4601 Fragments arranged by size: 4601 1028 21 12 12 9 9 3 3 BseYI C'CCAG_C Cuts at: 2836 4901 5397 2836 Size: 2065 496 3137 Fragments arranged by size: 3137 2065 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2116 2576 1163 Size: 573 380 460 4285 Fragments arranged by size: 4285 573 460 380 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2127 2155 2181 2910 3148 3909 Size: 275 186 126 28 26 729 238 761 Cuts at: 3909 4937 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 729 275 238 186 126 28 26 BsiHKAI G_wGCw'C Cuts at: 3759 3844 4915 3759 Size: 85 1071 4542 Fragments arranged by size: 4542 1071 85 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 BsmBI CGTCTCn'nnnn_ Cuts at: 2032 2032 Size: 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3017 3759 3844 4915 1391 Size: 399 1227 742 85 1071 2174 Fragments arranged by size: 2174 1227 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 3587 5317 3587 Size: 1730 3968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2548 2802 3053 844 Size: 1704 254 251 3489 Fragments arranged by size: 3489 1704 254 251 BsrBI CCG'CTC Cuts at: 1394 1823 2097 2860 3585 1394 Size: 429 274 763 725 3507 Fragments arranged by size: 3507 763 725 429 274 BsrDI GCAATG_nn' Cuts at: 65 1248 2713 4193 4367 65 Size: 1183 1465 1480 174 1396 Fragments arranged by size: 1480 1465 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2126 2180 4342 4529 1700 Size: 110 316 54 2162 187 2869 Fragments arranged by size: 2869 2162 316 187 110 54 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5698 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstYI r'GATC_y Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693 Size: 1056 367 385 17 1452 86 12 346 Cuts at: 5693 1972 Size: 1977 Fragments arranged by size: 1977 1452 1056 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2874 513 Size: 552 939 870 3337 Fragments arranged by size: 3337 939 870 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2932 487 Size: 683 56 1706 3253 Fragments arranged by size: 3253 1706 683 56 BtsI GCAGTG_nn' Cuts at: 3214 4008 4028 3214 Size: 794 20 4884 Fragments arranged by size: 4884 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2350 2731 4705 809 Size: 507 1034 381 1974 1802 Fragments arranged by size: 1974 1802 1034 507 381 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2808 2871 Size: 54 1425 50 275 18 348 630 63 Cuts at: 2871 2907 3145 4034 5663 8 Size: 36 238 889 1629 43 Fragments arranged by size: 1629 1425 889 630 348 275 238 63 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2178 2907 3145 1537 Size: 275 366 729 238 4090 Fragments arranged by size: 4090 729 366 275 238 EarI CTCTTCn'nnn_ Cuts at: 1353 2572 3628 1353 Size: 1219 1056 3423 Fragments arranged by size: 3423 1219 1056 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2199 2211 2220 2232 2241 2271 Size: 1064 138 610 12 9 12 9 30 Cuts at: 2271 2878 4285 4659 4805 387 Size: 607 1407 374 146 1280 Fragments arranged by size: 1407 1280 1064 610 607 374 146 138 30 12 12 9 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2286 2489 2926 3761 4358 5145 Size: 393 479 175 203 437 835 597 787 Cuts at: 5145 5413 1239 Size: 268 1524 Fragments arranged by size: 1524 835 787 597 479 437 393 268 203 175 EcoNI CCTnn'n_nnAGG Cuts at: 2543 2543 Size: 5698 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 2278 2307 2772 4845 1678 Size: 600 29 465 2073 2531 Fragments arranged by size: 2531 2073 600 465 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2416 2448 2479 2511 2844 Size: 32 135 32 844 32 31 32 333 Cuts at: 2844 2876 4339 4371 4413 4445 1373 Size: 32 1463 32 42 32 2626 Fragments arranged by size: 2626 1463 844 333 135 42 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2674 3042 3288 669 Size: 988 281 736 368 246 3079 Fragments arranged by size: 3079 988 736 368 281 246 HindIII A'AGCT_T Cuts at: 748 2649 748 Size: 1901 3797 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KasI G'GCGC_C Cuts at: 2303 2303 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2414 2523 4812 4996 1305 Size: 1109 109 2289 184 2007 Fragments arranged by size: 2289 2007 1109 184 109 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3876 Fragments arranged by size: 3876 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2616 3656 4015 4174 518 Size: 648 1450 1040 359 159 2042 Fragments arranged by size: 2042 1450 1040 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2104 2755 2761 2876 3114 3791 4939 5184 Size: 498 651 6 115 238 677 1148 245 Cuts at: 5184 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 651 498 245 238 115 6 NaeI GCC'GGC Cuts at: 2182 2182 Size: 5698 NarI GG'CG_CC Cuts at: 2304 2304 Size: 5698 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4207 Fragments arranged by size: 4207 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NgoMIV G'CCGG_C Cuts at: 2180 2180 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 2907 3145 2907 Size: 238 5460 NspI r_CATG'y Cuts at: 1132 1229 3056 4601 1132 Size: 97 1827 1545 2229 Fragments arranged by size: 2229 1827 1545 97 PasI CC'CwG_GG Cuts at: 1348 2989 1348 Size: 1641 4057 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 2533 1108 Size: 1425 4273 PfoI T'CCnGG_A Cuts at: 2286 2286 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PstI C_TGCA'G Cuts at: 830 2760 3050 830 Size: 1930 290 3478 Fragments arranged by size: 3478 1930 290 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2755 2761 2755 Size: 6 5692 SacII CC_GC'GG Cuts at: 2877 2877 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SapI GCTCTTCn'nnn_ Cuts at: 2572 2572 Size: 5698 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2756 3046 4181 4862 5053 826 Size: 219 1711 290 1135 681 191 1471 Fragments arranged by size: 1711 1471 1135 681 290 219 191 SfoI GGC'GCC Cuts at: 2305 2305 Size: 5698 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3802 4703 4965 820 Size: 197 926 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2004 2115 3137 513 Size: 552 939 111 1022 3074 Fragments arranged by size: 3074 1022 939 552 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3725 3742 3895 4080 4525 325 Size: 1255 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3944 5656 96 Size: 274 80 33 51 1443 1967 1712 138 Fragments arranged by size: 1967 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5658 5690 5658 Size: 32 5666 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2446 2446 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI