################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BciVI BclI BfaI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HindIII HpaI KpnI KspI MaeI MaeIII MamI MfeI MluI MroI MseI MslI MunI MvnI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 480 GACGTC GACGT^C AccI 157 GTCGAC GT^[AC][GT]AC AfaI 42 GTAC GT^AC AlwI 183 GATCC GGATC(4/5) AocI 519 CCTGAGG CC^T[ACGT]AGG Aor51HI 107 AGCGCT AGC^GCT AvaII 27 GGACC G^G[AT]CC BalI 527 TGGCCA TGG^CCA BbeI 121 GGCGCC GGCGC^C BbsI 171 GAAGAC GAAGAC(2/6) BcgI 602 GCAAGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 271 GCCAATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 209 AGATCT A^GATCT BlpI 448 GCTGAGC GC^T[ACGT]AGC BpmI 178 CTGGAG CTGGAG(16/14) Bpu1102I 448 GCTGAGC GC^T[ACGT]AGC BpuAI 171 GAAGAC GAAGAC(2/6) BsaI 420 GGTCTC GGTCTC(1/5) BsiEI 221 CGGCCG CG[GA][CT]^CG BsmBI 478 GAGACG CGTCTC(1/5) BsrBI 232 GAGCGG CCGCTC(-3/-3) BsrI 332 ACTGG ACTGG(1/-1) BstAPI 89 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 519 CCTGAGG CC^T[ACGT]AGG CelII 448 GCTGAGC GC^T[ACGT]AGC DsaI 530 CCACGG C^C[GA][CT]GG EagI 221 CGGCCG C^GGCCG EarI 573 GAAGAG CTCTTC(1/4) EclXI 221 CGGCCG C^GGCCG Eco47III 107 AGCGCT AGC^GCT Eco52I 221 CGGCCG C^GGCCG Eco81I 519 CCTGAGG CC^T[ACGT]AGG EcoNI 515 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 222 GGCCGGCC GGCCGG^CC HincII 157 GTCGAC GT[CT]^[GA]AC HindII 157 GTCGAC GT[CT]^[GA]AC HinfI 493 GACTC G^A[ACGT]TC KasI 121 GGCGCC G^GCGCC Ksp632I 573 GAAGAG CTCTTC(1/4) MluNI 527 TGGCCA TGG^CCA MscI 527 TGGCCA TGG^CCA NaeI 223 GCCGGC GCC^GGC NarI 121 GGCGCC GG^CGCC NgoMIV 223 GCCGGC G^CCGGC PaeR7I 495 CTCGAG C^TCGAG PleI 493 GACTC GAGTC(4/5) PpuMI 26 GGGACCC [GA]G^G[AT]CC[CT] PstI 205 CTGCAG CTGCA^G RsaI 42 GTAC GT^AC SalI 157 GTCGAC G^TCGAC SapI 573 GAAGAGC GCTCTTC(1/4) SfcI 205 CTGCAG C^T[GA][CT]AG SfoI 121 GGCGCC GGC^GCC SmaI 21 CCCGGG CCC^GGG SmlI 495 CTCGAG C^T[CT][GA]AG StuI 512 AGGCCT AGG^CCT XcmI 167 CCATGAAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 495 CTCGAG C^TCGAG XmaCI 21 CCCGGG C^CCGGG XmaI 21 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 376 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 439 CAGAGCCTG ApaI 12 GGGCCC GGGCC^C 555 GGGCCC AvaI 21 CCCGGG C^[CT]CG[GA]G 495 CTCGAG BanI 121 GGCGCC G^G[CT][GA]CC 324 GGTGCC BbvCI 402 CCTCAGC CCTCAGC(-5/-2) 430 GCTGAGG BseRI 306 GAGGAG GAGGAG(10/8) 508 GAGGAG BsgI 103 GTGCAG GTGCAG(16/14) 373 GTGCAG BsmAI 478 GAGAC GTCTC(1/5) 421 GTCTC BsmFI 26 GGGAC GGGAC(10/14) 215 GGGAC BsoBI 21 CCCGGG C^[CT]CG[GA]G 495 CTCGAG BsrFI 118 ACCGGC [GA]^CCGG[CT] 223 GCCGGC BstYI 182 AGATCC [GA]^GATC[CT] 209 AGATCT Cfr10I 118 ACCGGC [GA]^CCGG[CT] 223 GCCGGC EcoT14I 425 CCTTGG C^C[AT][AT]GG 488 CCAAGG MboII 171 GAAGA GAAGA(8/7) 573 GAAGA MflI 182 AGATCC [GA]^GATC[CT] 209 AGATCT MspA1I 376 CAGCTG C[AC]G^C[GT]G 592 CAGCTG NlaIII 168 CATG CATG^ 543 CATG PvuII 376 CAGCTG CAG^CTG 592 CAGCTG SacI 309 GAGCTC GAGCT^C 535 GAGCTC StyI 425 CCTTGG C^C[AT][AT]GG 488 CCAAGG TaqI 158 TCGA T^CGA 496 TCGA TspRI 267 CAGTG CA[GC]TG[ACGT][ACGT]^ 331 CACTG TthHB8I 158 TCGA T^CGA 496 TCGA XhoII 182 AGATCC [GA]^GATC[CT] 209 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AcyI AluI AspHI BanII BbvI BcnI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MboI MnlI MspI MvaI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TseI