################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI MvnI NarI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 1066 AAATTC [GA]^AATT[CT] AflIII 1025 ACATGT A^C[GA][CT]GT Alw44I 1034 GTGCAC G^TGCAC AlwNI 1306 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 122 GGGCCC GGGCC^C ApaLI 1034 GTGCAC G^TGCAC ApoI 1066 AAATTC [GA]^AATT[CT] Asp700I 1062 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 1314 GGATCC G^GATCC BcnI 17 CCCGG CC^[GC]GG BfaI 170 CTAG C^TAG Bsp1407I 1408 TGTACA T^GTACA BspLU11I 1025 ACATGT A^CATGT BsrFI 247 GCCGGC [GA]^CCGG[CT] BsrGI 1408 TGTACA T^GTACA BstAPI 583 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 204 GGTGACC G^GT[ACGT]ACC BstPI 204 GGTGACC G^GT[ACGT]ACC CfoI 493 GCGC GCG^C Cfr10I 247 GCCGGC [GA]^CCGG[CT] DrdI 180 GACCGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 204 GGTGACC G^GT[ACGT]ACC HhaI 493 GCGC GCG^C HinP1I 493 GCGC G^CGC HincII 643 GTCAAC GT[CT]^[GA]AC HindII 643 GTCAAC GT[CT]^[GA]AC MaeI 170 CTAG C^TAG MaeII 1099 ACGT A^CGT MseI 1462 TTAA T^TAA NaeI 247 GCCGGC GCC^GGC NciI 17 CCCGG CC^[GC]GG NcoI 875 CCATGG C^CATGG NdeI 963 CATATG CA^TATG NgoMIV 247 GCCGGC G^CCGGC PflMI 1305 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 113 GAGCTC GAGCT^C SexAI 1181 ACCTGGT A^CC[AT]GGT SphI 251 GCATGC GCATG^C SspBI 1408 TGTACA T^GTACA TfiI 1007 GAATC G^A[AT]TC Tru9I 1462 TTAA T^TAA Van91I 1305 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 704 CCATTCTGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1062 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 759 GACGCC G[GA]^CG[CT]C 1042 GGCGTC BalI 872 TGGCCA TGG^CCA 989 TGGCCA BanI 315 GGTGCC G^G[CT][GA]CC 1301 GGCACC BbvCI 75 CCTCAGC CCTCAGC(-5/-2) 774 CCTCAGC BsaHI 759 GACGCC G[GA]^CG[CT]C 1042 GGCGTC BstXI 149 CCAAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 461 CCACTGTCGTGG BstYI 839 AGATCC [GA]^GATC[CT] 1314 GGATCC DsaI 875 CCATGG C^C[GA][CT]GG 1376 CCGTGG EaeI 872 TGGCCA [CT]^GGCC[GA] 989 TGGCCA EcoT14I 536 CCAAGG C^C[AT][AT]GG 875 CCATGG HapII 18 CCGG C^CGG 248 CCGG HgaI 759 GACGC GACGC(5/10) 1043 GCGTC Hin1I 759 GACGCC G[GA]^CG[CT]C 1042 GGCGTC HpaII 18 CCGG C^CGG 248 CCGG MflI 839 AGATCC [GA]^GATC[CT] 1314 GGATCC MluNI 872 TGGCCA TGG^CCA 989 TGGCCA MscI 872 TGGCCA TGG^CCA 989 TGGCCA MslI 447 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 462 CACTGTCGTG MspI 18 CCGG C^CGG 248 CCGG NspI 251 GCATGC [GA]CATG^[CT] 1025 ACATGT PaeR7I 515 CTCGAG C^TCGAG 1124 CTCGAG PpuMI 803 GGGTCCT [GA]G^G[AT]CC[CT] 1355 GGGACCT PvuII 36 CAGCTG CAG^CTG 377 CAGCTG SfaNI 361 GCATC GCATC(5/9) 1331 GCATC SfcI 95 CTGTAG C^T[GA][CT]AG 732 CTACAG StyI 536 CCAAGG C^C[AT][AT]GG 875 CCATGG XhoI 515 CTCGAG C^TCGAG 1124 CTCGAG XhoII 839 AGATCC [GA]^GATC[CT] 1314 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbvI BmyI BpmI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoNI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SmlI StuI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I