(Circular) (Six-base) MAPSORT of: pFN21AE6487.seq Check: 9948 from: 1 to: 6226 pFN21AE6487 6226 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4036 278 Size: 53 83 186 936 2500 2468 Fragments arranged by size: 2500 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3569 1937 Size: 1632 4594 Acc65I G'GTAC_C Cuts at: 3547 3547 Size: 6226 AclI AA'CG_TT Cuts at: 1563 4353 4726 1563 Size: 2790 373 3063 Fragments arranged by size: 3063 2790 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 2360 2870 4289 5673 5941 1632 Size: 557 171 510 1419 1384 268 1917 Fragments arranged by size: 1917 1419 1384 557 510 268 171 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6029 AflIII A'CryG_T Cuts at: 1203 3030 5125 6102 1203 Size: 1827 2095 977 1327 Fragments arranged by size: 2095 1827 1327 977 AgeI A'CCGG_T Cuts at: 5057 5057 Size: 6226 AhdI GACnn_n'nnGTC Cuts at: 4955 4955 Size: 6226 AleI CACnn'nnGTG Cuts at: 1166 2471 1166 Size: 1305 4921 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6226 AlwNI CAG_nnn'CTG Cuts at: 3316 3969 5541 3316 Size: 653 1572 4001 Fragments arranged by size: 4001 1572 653 ApaI G_GGCC'C Cuts at: 2131 2131 Size: 6226 ApaLI G'TGCA_C Cuts at: 3039 4283 5439 3039 Size: 1244 1156 3826 Fragments arranged by size: 3826 1244 1156 ApoI r'AATT_y Cuts at: 1493 3071 3535 3761 3913 6022 1493 Size: 1578 464 226 152 2109 1697 Fragments arranged by size: 2109 1697 1578 464 226 152 AseI AT'TA_AT Cuts at: 160 1032 4780 160 Size: 872 3748 1606 Fragments arranged by size: 3748 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 AvaI C'yCGr_G Cuts at: 1870 1943 2520 2642 3129 3551 1870 Size: 73 577 122 487 422 4545 Fragments arranged by size: 4545 577 487 422 122 73 BamHI G'GATC_C Cuts at: 3319 3556 3923 3319 Size: 237 367 5622 Fragments arranged by size: 5622 367 237 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2320 3306 3547 5002 618 Size: 325 217 627 533 986 241 1455 1842 Fragments arranged by size: 1842 1455 986 627 533 325 241 217 BanII G_rGCy'C Cuts at: 1391 2122 2131 2481 2831 3545 1391 Size: 731 9 350 350 714 4072 Fragments arranged by size: 4072 731 714 350 350 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5255 BbvCI CC'TCA_GC Cuts at: 2081 2780 2081 Size: 699 5527 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4417 4451 1903 Size: 34 2480 34 3678 Fragments arranged by size: 3678 2480 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4118 5328 4118 Size: 1210 5016 BclI T'GATC_A Cuts at: 1548 1548 Size: 6226 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4837 136 Size: 107 122 71 631 605 3165 1525 Fragments arranged by size: 3165 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6221 6221 Size: 6226 BlpI GC'TnA_GC Cuts at: 3643 3643 Size: 6226 Bme1580I G_kGCm'C Cuts at: 1790 2131 2220 3043 4287 5443 1790 Size: 341 89 823 1244 1156 2573 Fragments arranged by size: 2573 1244 1156 823 341 89 BmgBI CAC'GTC Cuts at: 1318 3105 1318 Size: 1787 4439 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2795 3957 4915 Size: 416 53 83 399 24 1378 1162 958 Cuts at: 4915 442 Size: 1753 Fragments arranged by size: 1753 1378 1162 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6226 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3155 3155 Size: 6226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2372 3044 4886 1239 Size: 944 189 672 1842 2579 Fragments arranged by size: 2579 1842 944 672 189 Bpu10I CC'TnA_GC Cuts at: 2081 2780 2081 Size: 699 5527 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2932 4351 5216 5514 2932 Size: 1419 865 298 3644 Fragments arranged by size: 3644 1419 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2012 2177 2685 4889 882 Size: 617 513 165 508 2204 2219 Fragments arranged by size: 2219 2204 617 513 508 165 BsaAI yAC'GTr Cuts at: 493 1204 4017 493 Size: 711 2813 2702 Fragments arranged by size: 2813 2702 711 BsaBI GATnn'nnATC Cuts at: 6220 6220 Size: 6226 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2765 3048 4033 4415 Size: 53 83 186 936 1232 283 985 382 Cuts at: 4415 5050 275 Size: 635 1451 Fragments arranged by size: 1451 1232 985 936 635 382 283 186 83 53 BsaWI w'CCGG_w Cuts at: 4659 5057 5331 5478 5889 4659 Size: 398 274 147 411 4996 Fragments arranged by size: 4996 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2540 2570 3185 3215 1178 Size: 30 165 30 1137 30 615 30 4189 Fragments arranged by size: 4189 1137 615 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6226 BseYI C'CCAG_C Cuts at: 2062 3355 5429 5925 2062 Size: 1293 2074 496 2363 Fragments arranged by size: 2363 2074 1293 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2480 3011 3085 3439 1163 Size: 573 744 531 74 354 3950 Fragments arranged by size: 3950 744 573 531 354 74 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3676 4437 5465 1540 Size: 275 186 1675 761 1028 2301 Fragments arranged by size: 2301 1675 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2122 2635 3043 3234 4287 4372 5443 2122 Size: 513 408 191 1053 85 1071 2905 Fragments arranged by size: 2905 1071 1053 513 408 191 85 BsmI GAATG_Cn' Cuts at: 3737 3830 3737 Size: 93 6133 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2122 2131 2220 2481 2635 2831 3043 Size: 399 332 9 89 261 154 196 212 Cuts at: 3043 3234 3545 4287 4372 5443 1391 Size: 191 311 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 332 311 261 212 196 191 154 89 85 9 BspEI T'CCGG_A Cuts at: 5889 5889 Size: 6226 BspHI T'CATG_A Cuts at: 4115 5845 4115 Size: 1730 4496 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3033 3246 3372 3581 844 Size: 2189 213 126 209 3489 Fragments arranged by size: 3489 2189 213 209 126 BsrBI CCG'CTC Cuts at: 1394 1823 4113 1394 Size: 429 2290 3507 Fragments arranged by size: 3507 2290 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2661 2673 2814 4721 4895 65 Size: 1183 1413 12 141 1907 174 1396 Fragments arranged by size: 1907 1413 1396 1183 174 141 12 BsrFI r'CCGG_y Cuts at: 1700 1810 2252 4870 5057 1700 Size: 110 442 2618 187 2869 Fragments arranged by size: 2869 2618 442 187 110 BsrGI T'GTAC_A Cuts at: 96 3413 96 Size: 3317 2909 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6226 BssSI C'ACGA_G Cuts at: 4286 5298 4286 Size: 1012 5214 BstAPI GCAn_nnn'nTGC Cuts at: 794 2594 794 Size: 1800 4426 BstBI TT'CG_AA Cuts at: 5030 5030 Size: 6226 BstEII G'GTnAC_C Cuts at: 1184 2209 1184 Size: 1025 5201 BstXI CCAn_nnnn'nTGG Cuts at: 2161 2473 2161 Size: 312 5914 BstYI r'GATC_y Cuts at: 1972 2844 3319 3556 3923 4308 4325 5777 5863 Size: 872 475 237 367 385 17 1452 86 Cuts at: 5863 5875 6221 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 872 475 385 367 346 237 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2880 3381 513 Size: 552 939 876 501 3358 Fragments arranged by size: 3358 939 876 552 501 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5487 Fragments arranged by size: 5487 683 56 BtsI GCAGTG_nn' Cuts at: 2572 2574 3331 3390 3742 4536 4556 2572 Size: 2 757 59 352 794 20 4242 Fragments arranged by size: 4242 794 757 352 59 20 2 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6226 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2656 2691 3363 3398 529 Size: 35 2092 35 672 35 3357 Fragments arranged by size: 3357 2092 672 35 35 35 DraI TTT'AAA Cuts at: 3530 3530 Size: 6226 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2191 5233 809 Size: 507 875 3042 1802 Fragments arranged by size: 3042 1802 875 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2877 2994 3673 Size: 54 1425 50 275 18 1047 117 679 Cuts at: 3673 4562 6191 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1047 889 679 275 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3673 1537 Size: 275 1861 4090 Fragments arranged by size: 4090 1861 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4156 1353 Size: 2803 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4813 5187 5333 387 Size: 1064 138 3224 374 146 1280 Fragments arranged by size: 3224 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2120 2120 Size: 6226 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2189 2360 2372 2870 3044 4289 Size: 393 551 6 171 12 498 174 1245 Cuts at: 4289 4886 5673 5941 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1245 787 597 551 498 393 268 174 171 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 2453 3191 3254 2453 Size: 738 63 5425 Fragments arranged by size: 5425 738 63 EcoO109I rG'GnC_Cy Cuts at: 2127 2128 2809 3313 3361 3433 2127 Size: 1 681 504 48 72 4920 Fragments arranged by size: 4920 681 504 72 48 1 EcoRI G'AATT_C Cuts at: 3535 3913 3535 Size: 378 5848 FspI TGC'GCA Cuts at: 4732 4732 Size: 6226 HaeII r_GCGC'y Cuts at: 1678 5373 1678 Size: 3695 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2617 2649 2682 2714 2892 Size: 32 135 32 1045 32 33 32 178 Cuts at: 2892 2924 3185 3217 3420 3452 4867 4899 4941 Size: 32 261 32 203 32 1415 32 42 Cuts at: 4941 4973 1373 Size: 32 2626 Fragments arranged by size: 2626 1415 1045 261 203 178 135 42 33 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2650 3570 3816 669 Size: 988 281 712 920 246 3079 Fragments arranged by size: 3079 988 920 712 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6226 HpaI GTT'AAC Cuts at: 3816 3816 Size: 6226 KpnI G_GTAC'C Cuts at: 3551 3551 Size: 6226 MfeI C'AATT_G Cuts at: 3825 3825 Size: 6226 MluI A'CGCG_T Cuts at: 6102 6102 Size: 6226 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5340 5524 1305 Size: 4035 184 2007 Fragments arranged by size: 4035 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2879 2996 10 Size: 54 1425 343 1047 117 3240 Fragments arranged by size: 3240 1425 1047 343 117 54 MslI CAynn'nnrTG Cuts at: 518 1166 2456 2471 4184 4543 4702 518 Size: 648 1290 15 1713 359 159 2042 Fragments arranged by size: 2042 1713 1290 648 359 159 15 MspA1I CmG'CkG Cuts at: 1606 2029 2043 2384 2770 3642 4319 5467 5712 Size: 423 14 341 386 872 677 1148 245 Cuts at: 5712 1606 Size: 2120 Fragments arranged by size: 2120 1148 872 677 423 386 341 245 14 NaeI GCC'GGC Cuts at: 2254 2254 Size: 6226 NcoI C'CATG_G Cuts at: 513 1065 2004 2880 513 Size: 552 939 876 3859 Fragments arranged by size: 3859 939 876 552 NdeI CA'TA_TG Cuts at: 387 2969 387 Size: 2582 3644 NgoMIV G'CCGG_C Cuts at: 2252 2252 Size: 6226 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6226 NotI GC'GGCC_GC Cuts at: 3673 3673 Size: 6226 NspI r_CATG'y Cuts at: 1132 1229 2260 3034 3584 5129 1132 Size: 97 1031 774 550 1545 2229 Fragments arranged by size: 2229 1545 1031 774 550 97 PasI CC'CwG_GG Cuts at: 1348 2599 1348 Size: 1251 4975 PciI A'CATG_T Cuts at: 3030 5125 3030 Size: 2095 4131 PflMI CCAn_nnn'nTGG Cuts at: 1108 3316 1108 Size: 2208 4018 PmeI GTTT'AAAC Cuts at: 3530 3530 Size: 6226 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4295 4327 5808 5840 4295 Size: 32 1481 32 4681 Fragments arranged by size: 4681 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2809 3361 2809 Size: 552 5674 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6226 PsiI TTA'TAA Cuts at: 3796 3796 Size: 6226 PspOMI G'GGCC_C Cuts at: 2127 2127 Size: 6226 PspXI vC'TCGA_Gb Cuts at: 3129 3129 Size: 6226 PstI C_TGCA'G Cuts at: 830 3578 830 Size: 2748 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6226 PvuII CAG'CTG Cuts at: 2043 2384 2043 Size: 341 5885 SacI G_AGCT'C Cuts at: 2122 2122 Size: 6226 SalI G'TCGA_C Cuts at: 1936 3568 1936 Size: 1632 4594 SbfI CC_TGCA'GG Cuts at: 3578 3578 Size: 6226 ScaI AGT'ACT Cuts at: 4474 6186 4474 Size: 1712 4514 SexAI A'CCwGG_T Cuts at: 3186 3186 Size: 6226 SfcI C'TryA_G Cuts at: 826 1045 2100 2737 3574 4709 5390 5581 826 Size: 219 1055 637 837 1135 681 191 1471 Fragments arranged by size: 1471 1135 1055 837 681 637 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6226 SmaI CCC'GGG Cuts at: 1872 3553 1872 Size: 1681 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2520 2911 3129 4330 5231 5493 Size: 197 926 577 391 218 1201 901 262 Cuts at: 5493 820 Size: 1553 Fragments arranged by size: 1553 1201 926 901 577 391 262 218 197 SnaBI TAC'GTA Cuts at: 493 4017 493 Size: 3524 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6226 SphI G_CATG'C Cuts at: 1132 2260 3584 1132 Size: 1128 1324 3774 Fragments arranged by size: 3774 1324 1128 SspI AAT'ATT Cuts at: 5 52 4150 5 Size: 47 4098 2081 Fragments arranged by size: 4098 2081 47 StuI AGG'CCT Cuts at: 1517 2202 2235 3249 1517 Size: 685 33 1014 4494 Fragments arranged by size: 4494 1014 685 33 StyI C'CwwG_G Cuts at: 513 1065 2004 2541 2880 3665 513 Size: 552 939 537 339 785 3074 Fragments arranged by size: 3074 939 785 552 537 339 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2848 4253 4270 4423 4608 5053 325 Size: 1255 1268 1405 17 153 185 445 1498 Fragments arranged by size: 1498 1405 1268 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2937 3413 4472 Size: 274 80 33 51 1443 960 476 1059 Cuts at: 4472 6184 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1059 960 476 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2540 2572 3033 3065 6186 6218 2540 Size: 32 461 32 3121 32 2548 Fragments arranged by size: 3121 2548 461 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6226 XbaI T'CTAG_A Cuts at: 3562 3562 Size: 6226 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2716 2716 Size: 6226 XhoI C'TCGA_G Cuts at: 1943 2520 3129 1943 Size: 577 609 5040 Fragments arranged by size: 5040 609 577 XmaI C'CCGG_G Cuts at: 1870 3551 1870 Size: 1681 4545 XmnI GAAnn'nnTTC Cuts at: 3071 4355 3071 Size: 1284 4942 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4034 276 Size: 53 83 186 936 2500 2468 Fragments arranged by size: 2500 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI