(Linear) (Six-base) MAPSORT of: KIEE6487.seq Check: 208 from: 1 to: 1521 KIEE6487.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 355 865 1521 Size: 184 171 510 656 Fragments arranged by size: 656 510 184 171 AflIII A'CryG_T Cuts at: 0 1025 1521 Size: 1025 496 AleI CACnn'nnGTG Cuts at: 0 466 1521 Size: 466 1055 AlwNI CAG_nnn'CTG Cuts at: 0 1311 1521 Size: 1311 210 ApaI G_GGCC'C Cuts at: 0 126 1521 Size: 126 1395 ApaLI G'TGCA_C Cuts at: 0 1034 1521 Size: 1034 487 ApoI r'AATT_y Cuts at: 0 1066 1521 Size: 1066 455 AvaI C'yCGr_G Cuts at: 0 515 637 1124 1521 Size: 515 122 487 397 Fragments arranged by size: 515 487 397 122 BamHI G'GATC_C Cuts at: 0 1314 1521 Size: 1314 207 BanI G'GyrC_C Cuts at: 0 315 1301 1521 Size: 315 986 220 Fragments arranged by size: 986 315 220 BanII G_rGCy'C Cuts at: 0 117 126 476 826 1521 Size: 117 9 350 350 695 Fragments arranged by size: 695 350 350 117 9 BbvCI CC'TCA_GC Cuts at: 0 76 775 1521 Size: 76 699 746 Fragments arranged by size: 746 699 76 Bme1580I G_kGCm'C Cuts at: 0 126 215 1038 1521 Size: 126 89 823 483 Fragments arranged by size: 823 483 126 89 BmgBI CAC'GTC Cuts at: 0 1100 1521 Size: 1100 421 BmrI ACTGGGnnnn_n' Cuts at: 0 790 1521 Size: 790 731 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1150 1521 Size: 1150 371 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 367 1039 1521 Size: 178 189 672 482 Fragments arranged by size: 672 482 189 178 Bpu10I CC'TnA_GC Cuts at: 0 76 775 1521 Size: 76 699 746 Fragments arranged by size: 746 699 76 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 927 1521 Size: 927 594 BsaI GGTCTCn'nnnn_ Cuts at: 0 7 172 680 1521 Size: 7 165 508 841 Fragments arranged by size: 841 508 165 7 BsaHI Gr'CG_yC Cuts at: 0 760 1043 1521 Size: 760 283 478 Fragments arranged by size: 760 478 283 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 535 565 1180 1210 1521 Size: 535 30 615 30 311 Fragments arranged by size: 615 535 311 30 30 BseYI C'CCAG_C Cuts at: 0 57 1350 1521 Size: 57 1293 171 Fragments arranged by size: 1293 171 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 1006 1080 1434 1521 Size: 475 531 74 354 87 Fragments arranged by size: 531 475 354 87 74 BsiHKAI G_wGCw'C Cuts at: 0 117 630 1038 1229 1521 Size: 117 513 408 191 292 Fragments arranged by size: 513 408 292 191 117 Bsp1286I G_dGCh'C Cuts at: 0 117 126 215 476 630 826 1038 1229 Size: 117 9 89 261 154 196 212 191 Cuts at: 1229 1521 Size: 292 Fragments arranged by size: 292 261 212 196 191 154 117 89 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1028 1241 1367 1521 Size: 1028 213 126 154 Fragments arranged by size: 1028 213 154 126 BsrDI GCAATG_nn' Cuts at: 0 656 668 809 1521 Size: 656 12 141 712 Fragments arranged by size: 712 656 141 12 BsrFI r'CCGG_y Cuts at: 0 247 1521 Size: 247 1274 BsrGI T'GTAC_A Cuts at: 0 1408 1521 Size: 1408 113 BstAPI GCAn_nnn'nTGC Cuts at: 0 589 1521 Size: 589 932 BstEII G'GTnAC_C Cuts at: 0 204 1521 Size: 204 1317 BstXI CCAn_nnnn'nTGG Cuts at: 0 156 468 1521 Size: 156 312 1053 Fragments arranged by size: 1053 312 156 BstYI r'GATC_y Cuts at: 0 839 1314 1521 Size: 839 475 207 Fragments arranged by size: 839 475 207 BtgI C'CryG_G Cuts at: 0 875 1376 1521 Size: 875 501 145 Fragments arranged by size: 875 501 145 BtsI GCAGTG_nn' Cuts at: 0 567 569 1326 1385 1521 Size: 567 2 757 59 136 Fragments arranged by size: 757 567 136 59 2 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 651 686 1358 1393 1521 Size: 651 35 672 35 128 Fragments arranged by size: 672 651 128 35 35 DrdI GACnn_nn'nnGTC Cuts at: 0 186 1521 Size: 186 1335 EaeI y'GGCC_r Cuts at: 0 872 989 1521 Size: 872 117 532 Fragments arranged by size: 872 532 117 EcoICRI GAG'CTC Cuts at: 0 115 1521 Size: 115 1406 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 184 355 367 865 1039 1521 Size: 178 6 171 12 498 174 482 Fragments arranged by size: 498 482 178 174 171 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 448 1186 1249 1521 Size: 448 738 63 272 Fragments arranged by size: 738 448 272 63 EcoO109I rG'GnC_Cy Cuts at: 0 122 123 804 1308 1356 1428 1521 Size: 122 1 681 504 48 72 93 Fragments arranged by size: 681 504 122 93 72 48 1 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 612 644 677 709 887 919 1180 1212 Size: 612 32 33 32 178 32 261 32 Cuts at: 1212 1415 1447 1521 Size: 203 32 74 Fragments arranged by size: 612 261 203 178 74 33 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 645 1521 Size: 645 876 MscI TGG'CCA Cuts at: 0 874 991 1521 Size: 874 117 530 Fragments arranged by size: 874 530 117 MslI CAynn'nnrTG Cuts at: 0 451 466 1521 Size: 451 15 1055 Fragments arranged by size: 1055 451 15 MspA1I CmG'CkG Cuts at: 0 24 38 379 765 1521 Size: 24 14 341 386 756 Fragments arranged by size: 756 386 341 24 14 NaeI GCC'GGC Cuts at: 0 249 1521 Size: 249 1272 NcoI C'CATG_G Cuts at: 0 875 1521 Size: 875 646 NdeI CA'TA_TG Cuts at: 0 964 1521 Size: 964 557 NgoMIV G'CCGG_C Cuts at: 0 247 1521 Size: 247 1274 NspI r_CATG'y Cuts at: 0 255 1029 1521 Size: 255 774 492 Fragments arranged by size: 774 492 255 PasI CC'CwG_GG Cuts at: 0 594 1521 Size: 594 927 PciI A'CATG_T Cuts at: 0 1025 1521 Size: 1025 496 PflMI CCAn_nnn'nTGG Cuts at: 0 1311 1521 Size: 1311 210 PpuMI rG'GwC_Cy Cuts at: 0 804 1356 1521 Size: 804 552 165 Fragments arranged by size: 804 552 165 PspOMI G'GGCC_C Cuts at: 0 122 1521 Size: 122 1399 PspXI vC'TCGA_Gb Cuts at: 0 1124 1521 Size: 1124 397 PvuII CAG'CTG Cuts at: 0 38 379 1521 Size: 38 341 1142 Fragments arranged by size: 1142 341 38 SacI G_AGCT'C Cuts at: 0 117 1521 Size: 117 1404 SexAI A'CCwGG_T Cuts at: 0 1181 1521 Size: 1181 340 SfcI C'TryA_G Cuts at: 0 95 732 1521 Size: 95 637 789 Fragments arranged by size: 789 637 95 SmlI C'TyrA_G Cuts at: 0 515 906 1124 1521 Size: 515 391 218 397 Fragments arranged by size: 515 397 391 218 SphI G_CATG'C Cuts at: 0 255 1521 Size: 255 1266 StuI AGG'CCT Cuts at: 0 197 230 1244 1521 Size: 197 33 1014 277 Fragments arranged by size: 1014 277 197 33 StyI C'CwwG_G Cuts at: 0 536 875 1521 Size: 536 339 646 Fragments arranged by size: 646 536 339 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 843 1521 Size: 843 678 TatI w'GTAC_w Cuts at: 0 932 1408 1521 Size: 932 476 113 Fragments arranged by size: 932 476 113 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 535 567 1028 1060 1521 Size: 535 32 461 32 461 Fragments arranged by size: 535 461 461 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 711 1521 Size: 711 810 XhoI C'TCGA_G Cuts at: 0 515 1124 1521 Size: 515 609 397 Fragments arranged by size: 609 515 397 XmnI GAAnn'nnTTC Cuts at: 0 1066 1521 Size: 1066 455 Enzymes that do cut: AcuI AflIII AleI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbvCI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DrdI EaeI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PciI PflMI PpuMI PspOMI PspXI PvuII SacI SexAI SfcI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI DraI DraIII EagI EarI EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmaI ZraI