################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaI BseAI BsiEI BsiHKAI BsiWI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboII MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 144 GACGCC G[GA]^CG[CT]C AlwI 219 GATCC GGATC(4/5) Aor51HI 382 AGCGCT AGC^GCT BclI 248 TGATCA T^GATCA BglI 73 GCCATTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 631 CTGGAG CTGGAG(16/14) BsaAI 495 CACGTA [CT]AC^GT[GA] BsaHI 144 GACGCC G[GA]^CG[CT]C BsaWI 150 TCCGGT [AT]^CCGG[AT] BsgI 257 GTGCAG GTGCAG(16/14) BsmAI 142 GAGAC GTCTC(1/5) BsmBI 142 GAGACG CGTCTC(1/5) BsmFI 132 GTCCC GGGAC(10/14) BsmI 225 GCATTC GAATGC(1/-1) BspLU11I 534 ACATGT A^CATGT BsrBI 160 GAGCGG CCGCTC(-3/-3) BssKI 620 CCAGG ^CC[ACGT]GG BstNI 620 CCAGG CC^[AT]GG BstXI 444 CCACAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 135 CCGTGG C^C[GA][CT]GG Eco47III 382 AGCGCT AGC^GCT EcoRII 620 CCAGG ^CC[AT]GG EcoT22I 24 ATGCAT ATGCA^T FauI 30 CCCGC CCCGC(4/6) FbaI 248 TGATCA T^GATCA HaeII 382 AGCGCT [GA]GCGC^[CT] HgaI 144 GACGC GACGC(5/10) Hin1I 144 GACGCC G[GA]^CG[CT]C MaeII 496 ACGT A^CGT MslI 445 CACAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 620 CCAGG CC^[AT]GG NlaIV 109 GGGGCC GG[ACGT]^[ACGT]CC NsiI 24 ATGCAT ATGCA^T PflMI 620 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 173 GAGTC GAGTC(4/5) PpuMI 92 AGGACCT [GA]G^G[AT]CC[CT] ScrFI 620 CCAGG CC^[ACGT]GG SmlI 412 CTCAAG C^T[CT][GA]AG TaqI 350 TCGA T^CGA Tsp45I 440 GTGAC ^GT[GC]AC Tsp509I 573 AATT ^AATT TspRI 103 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 350 TCGA T^CGA Van91I 620 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 620 CCAGGGTTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AflIII 494 ACACGT A^C[GA][CT]GT 534 ACATGT AvaII 93 GGACC G^G[AT]CC 577 GGTCC BglII 303 AGATCT A^GATCT 521 AGATCT BseRI 262 GAGGAG GAGGAG(10/8) 343 GAGGAG CfoI 82 GCGC GCG^C 383 GCGC DraII 92 AGGACCT [GA]G^G[ACGT]CC[CT] 125 AGGCCCT EcoO109I 92 AGGACCT [GA]G^G[ACGT]CC[CT] 125 AGGCCCT HapII 34 CCGG C^CGG 151 CCGG HhaI 82 GCGC GCG^C 383 GCGC HinP1I 82 GCGC G^CGC 383 GCGC HpaII 34 CCGG C^CGG 151 CCGG MspI 34 CCGG C^CGG 151 CCGG NspI 22 ACATGC [GA]CATG^[CT] 534 ACATGT TfiI 291 GAATC G^A[AT]TC 464 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BsrI BstF5I BstYI Cac8I Cfr13I DdeI DpnII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MflI MnlI MseI MwoI NdeII NlaIII Sau3AI Sau96I SfaNI Tru9I TseI XhoII