(Circular) (Six-base) MAPSORT of: pFN21AE2008.seq Check: 847 from: 1 to: 6454 pFN21AE2008 6454 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2669 2737 2669 Size: 68 6386 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4264 278 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2090 3642 3797 1937 Size: 153 1552 155 4594 Fragments arranged by size: 4594 1552 155 153 Acc65I G'GTAC_C Cuts at: 3775 3775 Size: 6454 AclI AA'CG_TT Cuts at: 1563 4581 4954 1563 Size: 3018 373 3063 Fragments arranged by size: 3063 3018 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2053 2197 3456 3768 4517 5901 6169 1632 Size: 421 144 1259 312 749 1384 268 1917 Fragments arranged by size: 1917 1384 1259 749 421 312 268 144 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6257 AflIII A'CryG_T Cuts at: 1203 2364 3682 5353 6330 1203 Size: 1161 1318 1671 977 1327 Fragments arranged by size: 1671 1327 1318 1161 977 AgeI A'CCGG_T Cuts at: 5285 5285 Size: 6454 AhdI GACnn_n'nnGTC Cuts at: 5183 5183 Size: 6454 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6454 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6454 AlwNI CAG_nnn'CTG Cuts at: 2028 4197 5769 2028 Size: 2169 1572 2713 Fragments arranged by size: 2713 2169 1572 ApaLI G'TGCA_C Cuts at: 4511 5667 4511 Size: 1156 5298 ApoI r'AATT_y Cuts at: 1493 2098 2843 3763 3989 4141 6250 1493 Size: 605 745 920 226 152 2109 1697 Fragments arranged by size: 2109 1697 920 745 605 226 152 AseI AT'TA_AT Cuts at: 160 1032 3399 5008 160 Size: 872 2367 1609 1606 Fragments arranged by size: 2367 1609 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 AvaI C'yCGr_G Cuts at: 1870 1943 3779 1870 Size: 73 1836 4545 Fragments arranged by size: 4545 1836 73 BamHI G'GATC_C Cuts at: 3784 4151 3784 Size: 367 6087 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2374 3775 5230 618 Size: 325 217 627 587 1401 1455 1842 Fragments arranged by size: 1842 1455 1401 627 587 325 217 BanII G_rGCy'C Cuts at: 1391 3773 1391 Size: 2382 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2059 2490 3140 928 Size: 971 160 431 650 4242 Fragments arranged by size: 4242 971 650 431 160 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4645 4679 1903 Size: 34 2708 34 3678 Fragments arranged by size: 3678 2708 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4346 5556 4346 Size: 1210 5244 BclI T'GATC_A Cuts at: 1548 1548 Size: 6454 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5065 136 Size: 107 122 71 631 605 3393 1525 Fragments arranged by size: 3393 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6449 6449 Size: 6454 BlpI GC'TnA_GC Cuts at: 3871 3871 Size: 6454 Bme1580I G_kGCm'C Cuts at: 1790 4515 5671 1790 Size: 2725 1156 2573 Fragments arranged by size: 2725 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2192 2579 3276 Size: 416 53 83 399 24 775 387 697 Cuts at: 3276 4185 5143 442 Size: 909 958 1753 Fragments arranged by size: 1753 958 909 775 697 416 399 387 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3081 1056 Size: 2025 4429 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2639 3497 5114 1239 Size: 1400 858 1617 2579 Fragments arranged by size: 2579 1617 1400 858 Bpu10I CC'TnA_GC Cuts at: 2815 2815 Size: 6454 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4579 5444 5742 4579 Size: 865 298 5291 Fragments arranged by size: 5291 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3614 5117 882 Size: 617 2115 1503 2219 Fragments arranged by size: 2219 2115 1503 617 BsaAI yAC'GTr Cuts at: 493 1204 4245 493 Size: 711 3041 2702 Fragments arranged by size: 3041 2702 711 BsaBI GATnn'nnATC Cuts at: 6448 6448 Size: 6454 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4261 4643 5278 275 Size: 53 83 186 936 2728 382 635 1451 Fragments arranged by size: 2728 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4887 5285 5559 5706 6117 4887 Size: 398 274 147 411 5224 Fragments arranged by size: 5224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2541 2571 1178 Size: 30 165 30 1138 30 5061 Fragments arranged by size: 5061 1138 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3602 1186 Size: 2416 4038 BseYI C'CCAG_C Cuts at: 5657 6153 5657 Size: 496 5958 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 1163 Size: 573 522 5359 Fragments arranged by size: 5359 573 522 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3904 4665 5693 1540 Size: 275 186 1903 761 1028 2301 Fragments arranged by size: 2301 1903 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4515 4600 5671 4515 Size: 85 1071 5298 Fragments arranged by size: 5298 1071 85 BsmI GAATG_Cn' Cuts at: 3091 3965 4058 3091 Size: 874 93 5487 Fragments arranged by size: 5487 874 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3773 4515 4600 5671 1391 Size: 399 1983 742 85 1071 2174 Fragments arranged by size: 2174 1983 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6117 6117 Size: 6454 BspHI T'CATG_A Cuts at: 4343 6073 4343 Size: 1730 4724 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2669 2737 3396 3809 844 Size: 1825 68 659 413 3489 Fragments arranged by size: 3489 1825 659 413 68 BsrBI CCG'CTC Cuts at: 1394 1823 2552 4341 1394 Size: 429 729 1789 3507 Fragments arranged by size: 3507 1789 729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2926 4949 5123 65 Size: 1183 1678 2023 174 1396 Fragments arranged by size: 2023 1678 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5098 5285 1700 Size: 110 3288 187 2869 Fragments arranged by size: 3288 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3651 96 Size: 3555 2899 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6454 BssSI C'ACGA_G Cuts at: 4514 5526 4514 Size: 1012 5442 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6454 BstBI TT'CG_AA Cuts at: 5258 5258 Size: 6454 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6454 BstYI r'GATC_y Cuts at: 1972 2216 3784 4151 4536 4553 6005 6091 6103 Size: 244 1568 367 385 17 1452 86 12 Cuts at: 6103 6449 1972 Size: 346 1977 Fragments arranged by size: 1977 1568 1452 385 367 346 244 86 17 12 BstZ17I GTA'TAC Cuts at: 2091 2091 Size: 6454 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4963 Fragments arranged by size: 4963 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5715 Fragments arranged by size: 5715 683 56 BtsI GCAGTG_nn' Cuts at: 3364 3970 4764 4784 3364 Size: 606 794 20 5034 Fragments arranged by size: 5034 794 606 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6454 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6419 DraI TTT'AAA Cuts at: 2569 3758 2569 Size: 1189 5265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5461 809 Size: 507 4145 1802 Fragments arranged by size: 4145 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2689 3901 4790 Size: 54 1425 50 275 18 859 1212 889 Cuts at: 4790 6419 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1212 889 859 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3901 1537 Size: 275 2089 4090 Fragments arranged by size: 4090 2089 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 3432 4384 1353 Size: 692 1387 952 3423 Fragments arranged by size: 3423 1387 952 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2047 5041 5415 5561 387 Size: 1064 138 458 2994 374 146 1280 Fragments arranged by size: 2994 1280 1064 458 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2197 2639 3456 3497 3768 4517 Size: 393 421 144 442 817 41 271 749 Cuts at: 4517 5114 5901 6169 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 817 787 749 597 442 421 393 271 268 144 41 EcoO109I rG'GnC_Cy Cuts at: 3694 3694 Size: 6454 EcoRI G'AATT_C Cuts at: 3763 4141 3763 Size: 378 6076 FspI TGC'GCA Cuts at: 4960 4960 Size: 6454 HaeII r_GCGC'y Cuts at: 1678 5601 1678 Size: 3923 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2262 2294 2541 2573 5095 Size: 32 135 32 690 32 247 32 2522 Cuts at: 5095 5127 5169 5201 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2522 690 247 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3798 4044 669 Size: 988 281 1860 246 3079 Fragments arranged by size: 3079 1860 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6454 HpaI GTT'AAC Cuts at: 4044 4044 Size: 6454 KpnI G_GTAC'C Cuts at: 3779 3779 Size: 6454 MfeI C'AATT_G Cuts at: 4053 4053 Size: 6454 MluI A'CGCG_T Cuts at: 6330 6330 Size: 6454 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5568 5752 1305 Size: 4263 184 2007 Fragments arranged by size: 4263 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2691 10 Size: 54 1425 343 859 3773 Fragments arranged by size: 3773 1425 859 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2648 4412 4771 4930 518 Size: 648 1482 1764 359 159 2042 Fragments arranged by size: 2042 1764 1482 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3870 4547 5695 5940 1606 Size: 2264 677 1148 245 2120 Fragments arranged by size: 2264 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4963 Fragments arranged by size: 4963 939 552 NdeI CA'TA_TG Cuts at: 387 2107 387 Size: 1720 4734 NheI G'CTAG_C Cuts at: 1052 3077 1052 Size: 2025 4429 NotI GC'GGCC_GC Cuts at: 3901 3901 Size: 6454 NspI r_CATG'y Cuts at: 1132 1229 2347 3686 3812 5357 1132 Size: 97 1118 1339 126 1545 2229 Fragments arranged by size: 2229 1545 1339 1118 126 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6454 PciI A'CATG_T Cuts at: 3682 5353 3682 Size: 1671 4783 PflMI CCAn_nnn'nTGG Cuts at: 1108 2081 3342 1108 Size: 973 1261 4220 Fragments arranged by size: 4220 1261 973 PmeI GTTT'AAAC Cuts at: 3758 3758 Size: 6454 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4523 4555 6036 6068 4523 Size: 32 1481 32 4909 Fragments arranged by size: 4909 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3694 3694 Size: 6454 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6454 PsiI TTA'TAA Cuts at: 4024 4024 Size: 6454 PstI C_TGCA'G Cuts at: 830 3195 3806 830 Size: 2365 611 3478 Fragments arranged by size: 3478 2365 611 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6454 SalI G'TCGA_C Cuts at: 1936 3796 1936 Size: 1860 4594 SapI GCTCTTCn'nnn_ Cuts at: 2045 2045 Size: 6454 SbfI CC_TGCA'GG Cuts at: 3806 3806 Size: 6454 ScaI AGT'ACT Cuts at: 4702 6414 4702 Size: 1712 4742 SfcI C'TryA_G Cuts at: 826 1045 2739 3191 3802 4937 5618 5809 826 Size: 219 1694 452 611 1135 681 191 1471 Fragments arranged by size: 1694 1471 1135 681 611 452 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6454 SmaI CCC'GGG Cuts at: 1872 3781 1872 Size: 1909 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4558 5459 5721 820 Size: 197 926 2615 901 262 1553 Fragments arranged by size: 2615 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4245 493 Size: 3752 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6454 SphI G_CATG'C Cuts at: 1132 2347 3812 1132 Size: 1215 1465 3774 Fragments arranged by size: 3774 1465 1215 SspI AAT'ATT Cuts at: 5 52 4378 5 Size: 47 4326 2081 Fragments arranged by size: 4326 2081 47 StuI AGG'CCT Cuts at: 1517 2079 1517 Size: 562 5892 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 2080 2634 3893 513 Size: 552 939 9 67 554 1259 3074 Fragments arranged by size: 3074 1259 939 554 552 67 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2456 4481 4498 4651 4836 5281 325 Size: 1255 876 2025 17 153 185 445 1498 Fragments arranged by size: 2025 1498 1255 876 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2622 3651 4700 Size: 274 80 33 51 1443 645 1029 1049 Cuts at: 4700 6412 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1049 1029 645 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6414 6446 6414 Size: 32 6422 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6454 XbaI T'CTAG_A Cuts at: 3790 3790 Size: 6454 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2082 3339 2082 Size: 1257 5197 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6454 XmaI C'CCGG_G Cuts at: 1870 3779 1870 Size: 1909 4545 XmnI GAAnn'nnTTC Cuts at: 3321 4583 3321 Size: 1262 5192 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4262 276 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI