################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbrPI BbvCI BcgI BciVI BcnI BfaI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FseI FspI HapII HgaI HindIII HpaI HpaII KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MroI MspI MunI MvaI MvnI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) AcyI 439 GGCGCC G[GA]^CG[CT]C AflII 900 CTTAAG C^TTAAG AlwI 757 GGATC GGATC(4/5) Asp700I 586 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 371 TGGCCA TGG^CCA BanI 439 GGCGCC G^G[CT][GA]CC BbeI 439 GGCGCC GGCGC^C BbsI 69 GAAGAC GAAGAC(2/6) BclI 107 TGATCA T^GATCA BfrI 900 CTTAAG C^TTAAG BpuAI 69 GAAGAC GAAGAC(2/6) BsaHI 439 GGCGCC G[GA]^CG[CT]C BsgI 290 GTGCAG GTGCAG(16/14) Bsp1407I 176 TGTACA T^GTACA BspHI 401 TCATGA T^CATGA BsrGI 176 TGTACA T^GTACA BsrI 588 ACTGG ACTGG(1/-1) BstF5I 725 CATCC GGATG(2/0) BstYI 757 GGATCT [GA]^GATC[CT] CfoI 440 GCGC GCG^C Cfr13I 701 GGGCC G^G[ACGT]CC DsaI 113 CCGTGG C^C[GA][CT]GG EaeI 371 TGGCCA [CT]^GGCC[GA] EarI 542 CTCTTC CTCTTC(1/4) EcoT14I 689 CCAAGG C^C[AT][AT]GG FbaI 107 TGATCA T^GATCA FokI 725 CATCC GGATG(9/13) HaeII 439 GGCGCC [GA]GCGC^[CT] HhaI 440 GCGC GCG^C Hin1I 439 GGCGCC G[GA]^CG[CT]C HinP1I 440 GCGC G^CGC HincII 568 GTTGAC GT[CT]^[GA]AC HindII 568 GTTGAC GT[CT]^[GA]AC KasI 439 GGCGCC G^GCGCC Ksp632I 542 CTCTTC CTCTTC(1/4) MflI 757 GGATCT [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MspA1I 818 CAGCTG C[AC]G^C[GT]G NarI 439 GGCGCC GG^CGCC NlaIV 439 GGCGCC GG[ACGT]^[ACGT]CC PvuII 818 CAGCTG CAG^CTG RcaI 401 TCATGA T^CATGA Sau96I 701 GGGCC G^G[ACGT]CC SfaNI 724 GCATC GCATC(5/9) SfoI 439 GGCGCC GGC^GCC SspBI 176 TGTACA T^GTACA SspI 124 AATATT AAT^ATT StyI 689 CCAAGG C^C[AT][AT]GG XhoII 757 GGATCT [GA]^GATC[CT] XmnI 586 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 786 AAATTT [GA]^AATT[CT] 908 AAATTC ApoI 786 AAATTT [GA]^AATT[CT] 908 AAATTC BbvI 346 GCAGC GCAGC(8/12) 820 GCTGC BsaJI 113 CCGTGG C^C[ACGT][ACGT]GG 689 CCAAGG BsiYI 113 CCGTGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 363 CCGCTATGTGG BslI 113 CCGTGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 363 CCGCTATGTGG BsmI 422 GCATTC GAATGC(1/-1) 462 GCATTC Fnu4HI 346 GCAGC GC^[ACGT]GC 820 GCTGC HphI 57 TCACC GGTGA(8/7) 110 TCACC ItaI 346 GCAGC GC^[ACGT]GC 820 GCTGC MseI 643 TTAA T^TAA 901 TTAA MwoI 346 GCAGCAGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 442 GCCTTCATAGC PleI 72 GACTC GAGTC(4/5) 557 GACTC PstI 326 CTGCAG CTGCA^G 388 CTGCAG SfcI 326 CTGCAG C^T[GA][CT]AG 388 CTGCAG SmlI 900 CTTAAG C^T[CT][GA]AG 929 CTCAAG Tru9I 643 TTAA T^TAA 901 TTAA TseI 346 GCAGC G^C[AT]GC 820 GCTGC Tsp509I 787 AATT ^AATT 909 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AfaI AluI DdeI DpnII HaeIII HinfI MboI MboII MnlI MslI NdeII NlaIII RsaI Sau3AI TfiI TspRI