################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HindIII HpaII KasI KpnI KspI MaeII MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) AlwI 571 GATCC GGATC(4/5) Asp700I 269 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 778 GGACC G^G[AT]CC BalI 371 TGGCCA TGG^CCA BbvI 25 GCAGC GCAGC(8/12) BfaI 5 CTAG C^TAG BsaBI 594 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 742 GTCTC GTCTC(1/5) BsrDI 630 CATTGC GCAATG(2/0) BsrI 808 CCAGT ACTGG(1/-1) BssKI 195 CCTGG ^CC[ACGT]GG BstEII 108 GGTCACC G^GT[ACGT]ACC BstNI 195 CCTGG CC^[AT]GG BstPI 108 GGTCACC G^GT[ACGT]ACC BstXI 98 CCATCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 570 AGATCC [GA]^GATC[CT] DraII 439 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 113 CCATGG C^C[GA][CT]GG EaeI 371 TGGCCA [CT]^GGCC[GA] EarI 698 GAAGAG CTCTTC(1/4) EcoO109I 439 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 108 GGTCACC G^GT[ACGT]ACC EcoRI 31 GAATTC G^AATTC EcoRII 195 CCTGG ^CC[AT]GG Fnu4HI 25 GCAGC GC^[ACGT]GC HincII 495 GTTAAC GT[CT]^[GA]AC HindII 495 GTTAAC GT[CT]^[GA]AC HpaI 495 GTTAAC GTT^AAC ItaI 25 GCAGC GC^[ACGT]GC Ksp632I 698 GAAGAG CTCTTC(1/4) MaeI 5 CTAG C^TAG MamI 594 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 570 AGATCC [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MspA1I 26 CAGCTG C[AC]G^C[GT]G MvaI 195 CCTGG CC^[AT]GG NcoI 113 CCATGG C^CATGG NdeI 375 CATATG CA^TATG NlaIV 439 GGGGCC GG[ACGT]^[ACGT]CC PleI 557 GACTC GAGTC(4/5) PvuII 26 CAGCTG CAG^CTG ScrFI 195 CCTGG CC^[ACGT]GG TseI 25 GCAGC G^C[AT]GC Tsp45I 109 GTCAC ^GT[GC]AC XhoII 570 AGATCC [GA]^GATC[CT] XmnI 269 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AseI 141 ATTAAT AT^TAAT 580 ATTAAT AsnI 141 ATTAAT AT^TAAT 580 ATTAAT BsaJI 113 CCATGG C^C[ACGT][ACGT]GG 689 CCAAGG BsiYI 113 CCATGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 363 CCGCTATGTGG BslI 113 CCATGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 363 CCGCTATGTGG BsmI 422 GCATTC GAATGC(1/-1) 462 GCATTC Bsp1407I 176 TGTACA T^GTACA 565 TGTACA BspMI 458 ACCTGC ACCTGC(4/8) 506 GCAGGT BsrGI 176 TGTACA T^GTACA 565 TGTACA Cfr13I 440 GGGCC G^G[ACGT]CC 778 GGACC DpnII 571 GATC ^GATC 645 GATC EcoT14I 113 CCATGG C^C[AT][AT]GG 689 CCAAGG HphI 110 TCACC GGTGA(8/7) 522 TCACC MaeIII 109 GTCAC ^GT[ACGT]AC 229 GTTAC MboI 571 GATC ^GATC 645 GATC NdeII 571 GATC ^GATC 645 GATC PshBI 141 ATTAAT AT^TAAT 580 ATTAAT PstI 388 CTGCAG CTGCA^G 504 CTGCAG Sau3AI 571 GATC ^GATC 645 GATC Sau96I 440 GGGCC G^G[ACGT]CC 778 GGACC SfaNI 659 GCATC GCATC(5/9) 724 GCATC SspBI 176 TGTACA T^GTACA 565 TGTACA SspI 747 AATATT AAT^ATT 816 AATATT StyI 113 CCATGG C^C[AT][AT]GG 689 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AcsI AfaI AluI ApoI BbsI BpuAI BstF5I DdeI FokI HaeIII HgaI HinfI MboII MnlI MseI MslI MwoI NlaIII RsaI SfcI TfiI Tru9I Tsp509I TspRI