################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeI MaeII MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TseI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 192 GAATTT [GA]^AATT[CT] AfaI 177 GTAC GT^AC ApaI 757 GGGCCC GGGCC^C ApoI 192 GAATTT [GA]^AATT[CT] AseI 432 ATTAAT AT^TAAT AsnI 432 ATTAAT AT^TAAT Asp700I 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 725 GAGCTC G[AT]GC[AT]^C BbsI 455 GTCTTC GAAGAC(2/6) BclI 245 TGATCA T^GATCA BpuAI 455 GTCTTC GAAGAC(2/6) BsaBI 240 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 725 GAGCTC G[AT]GC[AT]^C BsmAI 822 GTCTC GTCTC(1/5) BsmFI 634 GGGAC GGGAC(10/14) BspMI 607 GCAGGT ACCTGC(4/8) BsrDI 20 CATTGC GCAATG(2/0) BssKI 84 CCTGG ^CC[ACGT]GG BstNI 84 CCTGG CC^[AT]GG DraII 757 GGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 326 CCACGG C^C[GA][CT]GG EarI 4 GAAGAG CTCTTC(1/4) EcoO109I 757 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoRII 84 CCTGG ^CC[AT]GG EcoRV 273 GATATC GAT^ATC EcoT14I 186 CCTTGG C^C[AT][AT]GG FbaI 245 TGATCA T^GATCA HaeIII 758 GGCC GG^CC HapII 426 CCGG C^CGG HpaII 426 CCGG C^CGG HphI 110 TCACC GGTGA(8/7) Ksp632I 4 GAAGAG CTCTTC(1/4) MamI 240 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 426 CCGG C^CGG MvaI 84 CCTGG CC^[AT]GG MwoI 346 GCAACAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PshBI 432 ATTAAT AT^TAAT RsaI 177 GTAC GT^AC SacI 725 GAGCTC GAGCT^C ScrFI 84 CCTGG CC^[ACGT]GG SfcI 867 CTACAG C^T[GA][CT]AG StyI 186 CCTTGG C^C[AT][AT]GG XmnI 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BsaJI 186 CCTTGG C^C[ACGT][ACGT]GG 326 CCACGG BsiYI 78 CCTGTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 113 CCATCATGGGG BslI 78 CCTGTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 113 CCATCATGGGG BsmI 286 GAATGC GAATGC(1/-1) 88 GCATTC Cfr13I 757 GGGCC G^G[ACGT]CC 758 GGCCC NlaIV 757 GGGCCC GG[ACGT]^[ACGT]CC 758 GGCCCC PleI 231 GACTC GAGTC(4/5) 820 GAGTC Sau96I 757 GGGCC G^G[ACGT]CC 758 GGCCC TfiI 123 GAATC G^A[AT]TC 571 GATTC Tsp45I 229 GTGAC ^GT[GC]AC 536 GTGAC Tsp509I 193 AATT ^AATT 615 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI BanII BmyI Bsp1286I BstF5I DdeI DpnII FokI HinfI MaeIII MboI MboII MnlI MseI MslI NdeI NdeII NlaIII Sau3AI SfaNI Tru9I TspRI