(Linear) (Six-base) MAPSORT of: KIBB9399.seq Check: 3226 from: 1 to: 5373 KIBB9399.orf With 182 enzymes: SgfI * April 12, 2013 15:25 .. AccI GT'mk_AC Cuts at: 0 2694 2967 3590 5373 Size: 2694 273 623 1783 Fragments arranged by size: 2694 1783 623 273 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 291 736 1998 2930 2980 4368 4853 5373 Size: 291 445 1262 932 50 1388 485 520 Fragments arranged by size: 1388 1262 932 520 485 445 291 50 AflIII A'CryG_T Cuts at: 0 2900 4790 5373 Size: 2900 1890 583 Fragments arranged by size: 2900 1890 583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1887 5373 Size: 1887 3486 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 4540 4572 5113 5145 5373 Size: 4540 32 541 32 228 Fragments arranged by size: 4540 541 228 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 448 962 1104 1278 1450 1689 3362 4576 Size: 448 514 142 174 172 239 1673 1214 Cuts at: 4576 4831 5373 Size: 255 542 Fragments arranged by size: 1673 1214 542 514 448 255 239 174 172 142 ApoI r'AATT_y Cuts at: 0 42 915 925 1020 1836 2779 3607 4287 Size: 42 873 10 95 816 943 828 680 Cuts at: 4287 5373 Size: 1086 Fragments arranged by size: 1086 943 873 828 816 680 95 42 10 AseI AT'TA_AT Cuts at: 0 998 3623 5373 Size: 998 2625 1750 Fragments arranged by size: 2625 1750 998 AvaI C'yCGr_G Cuts at: 0 852 5373 Size: 852 4521 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2690 2723 5373 Size: 2690 33 2650 Fragments arranged by size: 2690 2650 33 BamHI G'GATC_C Cuts at: 0 2937 4018 5373 Size: 2937 1081 1355 Fragments arranged by size: 2937 1355 1081 BanI G'GyrC_C Cuts at: 0 409 2748 3517 5373 Size: 409 2339 769 1856 Fragments arranged by size: 2339 1856 769 409 BanII G_rGCy'C Cuts at: 0 218 1121 1162 2100 2671 2846 5373 Size: 218 903 41 938 571 175 2527 Fragments arranged by size: 2527 938 903 571 218 175 41 BbsI GAAGACnn'nnnn_ Cuts at: 0 242 486 1122 1767 2022 2361 4495 5373 Size: 242 244 636 645 255 339 2134 878 Fragments arranged by size: 2134 878 645 636 339 255 244 242 BbvCI CC'TCA_GC Cuts at: 0 155 790 5373 Size: 155 635 4583 Fragments arranged by size: 4583 635 155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2073 2107 3828 3862 5373 Size: 2073 34 1721 34 1511 Fragments arranged by size: 2073 1721 1511 34 34 BclI T'GATC_A Cuts at: 0 2378 5373 Size: 2378 2995 BfrBI ATG'CAT Cuts at: 0 848 2344 3620 5373 Size: 848 1496 1276 1753 Fragments arranged by size: 1753 1496 1276 848 BglII A'GATC_T Cuts at: 0 4097 5373 Size: 4097 1276 BlpI GC'TnA_GC Cuts at: 0 3202 4669 5373 Size: 3202 1467 704 Fragments arranged by size: 3202 1467 704 Bme1580I G_kGCm'C Cuts at: 0 3216 5373 Size: 3216 2157 BmgBI CAC'GTC Cuts at: 0 712 5373 Size: 712 4661 BmrI ACTGGGnnnn_n' Cuts at: 0 13 509 1124 1677 3471 4215 5373 Size: 13 496 615 553 1794 744 1158 Fragments arranged by size: 1794 1158 744 615 553 496 13 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1564 2561 3125 5373 Size: 1564 997 564 2248 Fragments arranged by size: 2248 1564 997 564 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 3328 5293 5373 Size: 159 3169 1965 80 Fragments arranged by size: 3169 1965 159 80 Bpu10I CC'TnA_GC Cuts at: 0 155 790 2023 2819 4961 5373 Size: 155 635 1233 796 2142 412 Fragments arranged by size: 2142 1233 796 635 412 155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2569 3259 5134 5373 Size: 2569 690 1875 239 Fragments arranged by size: 2569 1875 690 239 BsaI GGTCTCn'nnnn_ Cuts at: 0 2668 4075 5373 Size: 2668 1407 1298 Fragments arranged by size: 2668 1407 1298 BsaBI GATnn'nnATC Cuts at: 0 94 5373 Size: 94 5279 BsaHI Gr'CG_yC Cuts at: 0 2723 5373 Size: 2723 2650 BsaWI w'CCGG_w Cuts at: 0 3437 5373 Size: 3437 1936 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1225 2698 2776 4447 5373 Size: 1225 1473 78 1671 926 Fragments arranged by size: 1671 1473 1225 926 78 BseYI C'CCAG_C Cuts at: 0 143 1271 5373 Size: 143 1128 4102 Fragments arranged by size: 4102 1128 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2592 2606 4421 5373 Size: 2592 14 1815 952 Fragments arranged by size: 2592 1815 952 14 BsiHKAI G_wGCw'C Cuts at: 0 218 1566 2024 2408 2846 3453 4361 5373 Size: 218 1348 458 384 438 607 908 1012 Fragments arranged by size: 1348 1012 908 607 458 438 384 218 BsmI GAATG_Cn' Cuts at: 0 2346 5373 Size: 2346 3027 Bsp1286I G_dGCh'C Cuts at: 0 218 1121 1162 1566 2024 2100 2408 2671 Size: 218 903 41 404 458 76 308 263 Cuts at: 2671 2846 3216 3453 4361 5373 Size: 175 370 237 908 1012 Fragments arranged by size: 1012 908 903 458 404 370 308 263 237 218 175 76 41 BspEI T'CCGG_A Cuts at: 0 3437 5373 Size: 3437 1936 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3140 3151 3534 4642 5373 Size: 3140 11 383 1108 731 Fragments arranged by size: 3140 1108 731 383 11 BsrBI CCG'CTC Cuts at: 0 39 5373 Size: 39 5334 BsrDI GCAATG_nn' Cuts at: 0 479 894 1883 2417 2451 4010 4796 5120 Size: 479 415 989 534 34 1559 786 324 Cuts at: 5120 5328 5373 Size: 208 45 Fragments arranged by size: 1559 989 786 534 479 415 324 208 45 34 BsrGI T'GTAC_A Cuts at: 0 4793 5373 Size: 4793 580 BssSI C'ACGA_G Cuts at: 0 209 1663 2868 4072 4439 5373 Size: 209 1454 1205 1204 367 934 Fragments arranged by size: 1454 1205 1204 934 367 209 BstAPI GCAn_nnn'nTGC Cuts at: 0 1570 2294 2755 5373 Size: 1570 724 461 2618 Fragments arranged by size: 2618 1570 724 461 BstBI TT'CG_AA Cuts at: 0 5232 5373 Size: 5232 141 BstEII G'GTnAC_C Cuts at: 0 3014 5250 5260 5269 5373 Size: 3014 2236 10 9 104 Fragments arranged by size: 3014 2236 104 10 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 587 1438 4729 5373 Size: 587 851 3291 644 Fragments arranged by size: 3291 851 644 587 BstYI r'GATC_y Cuts at: 0 576 1542 2937 4018 4097 5373 Size: 576 966 1395 1081 79 1276 Fragments arranged by size: 1395 1276 1081 966 576 79 BstZ17I GTA'TAC Cuts at: 0 2695 2968 3591 5373 Size: 2695 273 623 1782 Fragments arranged by size: 2695 1782 623 273 Bsu36I CC'TnA_GG Cuts at: 0 4420 5373 Size: 4420 953 BtgI C'CryG_G Cuts at: 0 580 1199 1310 1784 4045 5018 5373 Size: 580 619 111 474 2261 973 355 Fragments arranged by size: 2261 973 619 580 474 355 111 BtsI GCAGTG_nn' Cuts at: 0 3213 5040 5373 Size: 3213 1827 333 Fragments arranged by size: 3213 1827 333 ClaI AT'CG_AT Cuts at: 0 2511 4583 5373 Size: 2511 2072 790 Fragments arranged by size: 2511 2072 790 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 918 953 5373 Size: 918 35 4420 Fragments arranged by size: 4420 918 35 DraIII CAC_nnn'GTG Cuts at: 0 1570 5373 Size: 1570 3803 DrdI GACnn_nn'nnGTC Cuts at: 0 601 4303 5373 Size: 601 3702 1070 Fragments arranged by size: 3702 1070 601 EaeI y'GGCC_r Cuts at: 0 1742 1781 1996 2050 2169 3957 4410 5348 Size: 1742 39 215 54 119 1788 453 938 Cuts at: 5348 5373 Size: 25 Fragments arranged by size: 1788 1742 938 453 215 119 54 39 25 EarI CTCTTCn'nnn_ Cuts at: 0 826 1952 2419 3467 3910 4387 4636 4710 Size: 826 1126 467 1048 443 477 249 74 Cuts at: 4710 5117 5189 5373 Size: 407 72 184 Fragments arranged by size: 1126 1048 826 477 467 443 407 249 184 74 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 37 2506 5373 Size: 37 2469 2867 Fragments arranged by size: 2867 2469 37 EcoICRI GAG'CTC Cuts at: 0 216 2844 5373 Size: 216 2628 2529 Fragments arranged by size: 2628 2529 216 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 291 736 1998 2930 2980 3328 4368 Size: 159 132 445 1262 932 50 348 1040 Cuts at: 4368 4853 5293 5373 Size: 485 440 80 Fragments arranged by size: 1262 1040 932 485 445 440 348 159 132 80 50 EcoO109I rG'GnC_Cy Cuts at: 0 445 731 1704 4845 5373 Size: 445 286 973 3141 528 Fragments arranged by size: 3141 973 528 445 286 EcoRI G'AATT_C Cuts at: 0 925 5373 Size: 925 4448 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 339 1422 2601 5373 Size: 339 1083 1179 2772 Fragments arranged by size: 2772 1179 1083 339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 362 394 2890 2922 5016 5048 5373 Size: 362 32 2496 32 2094 32 325 Fragments arranged by size: 2496 2094 362 325 32 32 32 HincII GTy'rAC Cuts at: 0 762 3714 4788 5373 Size: 762 2952 1074 585 Fragments arranged by size: 2952 1074 762 585 HindIII A'AGCT_T Cuts at: 0 518 884 1087 5373 Size: 518 366 203 4286 Fragments arranged by size: 4286 518 366 203 MfeI C'AATT_G Cuts at: 0 77 5373 Size: 77 5296 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 86 2073 4399 5373 Size: 86 1987 2326 974 Fragments arranged by size: 2326 1987 974 86 MscI TGG'CCA Cuts at: 0 1744 1783 1998 2052 3959 5350 5373 Size: 1744 39 215 54 1907 1391 23 Fragments arranged by size: 1907 1744 1391 215 54 39 23 MslI CAynn'nnrTG Cuts at: 0 946 1529 1594 2355 2656 3385 3970 4801 Size: 946 583 65 761 301 729 585 831 Cuts at: 4801 5373 Size: 572 Fragments arranged by size: 946 831 761 729 585 583 572 301 65 MspA1I CmG'CkG Cuts at: 0 1278 3167 3796 5373 Size: 1278 1889 629 1577 Fragments arranged by size: 1889 1577 1278 629 NcoI C'CATG_G Cuts at: 0 580 1199 1310 1784 5018 5373 Size: 580 619 111 474 3234 355 Fragments arranged by size: 3234 619 580 474 355 111 NdeI CA'TA_TG Cuts at: 0 1206 5373 Size: 1206 4167 NsiI A_TGCA'T Cuts at: 0 850 2346 3622 5373 Size: 850 1496 1276 1751 Fragments arranged by size: 1751 1496 1276 850 NspI r_CATG'y Cuts at: 0 322 399 2160 2904 4623 4794 5373 Size: 322 77 1761 744 1719 171 579 Fragments arranged by size: 1761 1719 744 579 322 171 77 PasI CC'CwG_GG Cuts at: 0 2718 3113 5373 Size: 2718 395 2260 Fragments arranged by size: 2718 2260 395 PciI A'CATG_T Cuts at: 0 2900 4790 5373 Size: 2900 1890 583 Fragments arranged by size: 2900 1890 583 PflMI CCAn_nnn'nTGG Cuts at: 0 2048 3362 4283 4576 5046 5373 Size: 2048 1314 921 293 470 327 Fragments arranged by size: 2048 1314 921 470 327 293 PfoI T'CCnGG_A Cuts at: 0 175 633 3305 5373 Size: 175 458 2672 2068 Fragments arranged by size: 2672 2068 458 175 PpuMI rG'GwC_Cy Cuts at: 0 1704 4845 5373 Size: 1704 3141 528 Fragments arranged by size: 3141 1704 528 PshAI GACnn'nnGTC Cuts at: 0 2116 5373 Size: 2116 3257 PsiI TTA'TAA Cuts at: 0 3643 5373 Size: 3643 1730 PstI C_TGCA'G Cuts at: 0 1236 5373 Size: 1236 4137 PvuII CAG'CTG Cuts at: 0 1278 3167 3796 5373 Size: 1278 1889 629 1577 Fragments arranged by size: 1889 1577 1278 629 SacI G_AGCT'C Cuts at: 0 218 2846 5373 Size: 218 2628 2527 Fragments arranged by size: 2628 2527 218 SapI GCTCTTCn'nnn_ Cuts at: 0 826 1952 4387 5373 Size: 826 1126 2435 986 Fragments arranged by size: 2435 1126 986 826 SfcI C'TryA_G Cuts at: 0 440 1232 3087 5373 Size: 440 792 1855 2286 Fragments arranged by size: 2286 1855 792 440 SmlI C'TyrA_G Cuts at: 0 852 2548 3238 5149 5373 Size: 852 1696 690 1911 224 Fragments arranged by size: 1911 1696 852 690 224 SphI G_CATG'C Cuts at: 0 322 2160 4623 5373 Size: 322 1838 2463 750 Fragments arranged by size: 2463 1838 750 322 SspI AAT'ATT Cuts at: 0 533 4927 5373 Size: 533 4394 446 Fragments arranged by size: 4394 533 446 StuI AGG'CCT Cuts at: 0 172 2836 3459 5227 5373 Size: 172 2664 623 1768 146 Fragments arranged by size: 2664 1768 623 172 146 StyI C'CwwG_G Cuts at: 0 580 1199 1247 1310 1784 2100 4343 5018 Size: 580 619 48 63 474 316 2243 675 Cuts at: 5018 5255 5265 5351 5373 Size: 237 10 86 22 Fragments arranged by size: 2243 675 619 580 474 316 237 86 63 48 22 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 4843 5373 Size: 4843 530 TatI w'GTAC_w Cuts at: 0 1977 3274 4327 4793 5237 5373 Size: 1977 1297 1053 466 444 136 Fragments arranged by size: 1977 1297 1053 466 444 136 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2384 2416 3479 3511 5373 Size: 2384 32 1063 32 1862 Fragments arranged by size: 2384 1862 1063 32 32 Tth111I GACn'n_nGTC Cuts at: 0 4084 5373 Size: 4084 1289 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2686 5373 Size: 2686 2687 XhoI C'TCGA_G Cuts at: 0 852 5373 Size: 852 4521 XmnI GAAnn'nnTTC Cuts at: 0 1402 3230 3433 3904 5373 Size: 1402 1828 203 471 1469 Fragments arranged by size: 1828 1469 1402 471 203 Enzymes that do cut: AccI AcuI AflIII AlfI AloI AlwNI ApoI AseI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI PsiI PstI PvuII SacI SapI SfcI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI ApaI ApaLI AscI AsiSI AvrII BbeI BciVI BglI BmtI BsaAI BsaXI BsiEI BsiWI BsmBI BspHI BsrFI BssHII BtgZI DraI EagI EcoNI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI XbaI XmaI ZraI