################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AatII Acc65I AccI AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI BbrPI BcgI BsiWI BspDI BssHII Bst1107I BstZ17I ClaI CpoI Eam1105I Eco47III FseI HpaI KpnI KspI MfeI MluI MunI NotI NruI PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SgrAI SnaBI SwaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcyI 3826 GGCGCC G[GA]^CG[CT]C AspI 3568 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3422 TGCGCA TGC^GCA BbeI 3826 GGCGCC GGCGC^C BglII 618 AGATCT A^GATCT BlpI 3489 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3489 GCTCAGC GC^T[ACGT]AGC BsaAI 546 CACGTA [CT]AC^GT[GA] BsaBI 241 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3826 GGCGCC G[GA]^CG[CT]C BsgI 5599 CTGCAC GTGCAG(16/14) BsiEI 4979 CGGCCG CG[GA][CT]^CG BsmBI 3187 GAGACG CGTCTC(1/5) BsrFI 4981 GCCGGC [GA]^CCGG[CT] BssSI 387 CTCGTG CACGAG(-5/-1) BstEII 4066 GGTTACC G^GT[ACGT]ACC BstPI 4066 GGTTACC G^GT[ACGT]ACC CelII 3489 GCTCAGC GC^T[ACGT]AGC Cfr10I 4981 GCCGGC [GA]^CCGG[CT] DraI 4096 TTTAAA TTT^AAA EagI 4979 CGGCCG C^GGCCG EclXI 4979 CGGCCG C^GGCCG Eco52I 4979 CGGCCG C^GGCCG EcoNI 5205 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 4066 GGTTACC G^GT[ACGT]ACC EcoRI 4 GAATTC G^AATTC EcoT22I 3354 ATGCAT ATGCA^T FspI 3422 TGCGCA TGC^GCA HaeII 3826 GGCGCC [GA]GCGC^[CT] HgaI 584 GCGTC GACGC(5/10) Hin1I 3826 GGCGCC G[GA]^CG[CT]C KasI 3826 GGCGCC G^GCGCC MamI 241 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 4981 GCCGGC GCC^GGC NarI 3826 GGCGCC GG^CGCC NgoMIV 4981 GCCGGC G^CCGGC NheI 1293 GCTAGC G^CTAGC NsiI 3354 ATGCAT ATGCA^T PflFI 3568 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 4710 GCTCTTC GCTCTTC(1/4) SbfI 2727 CCTGCAGG CCTGCA^GG SfiI 1630 GGCCAGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3826 GGCGCC GGC^GCC SmaI 4136 CCCGGG CCC^GGG SpeI 1720 ACTAGT A^CTAGT Sse8387I 2727 CCTGCAGG CCTGCA^GG SspI 3716 AATATT AAT^ATT Tth111I 3568 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 243 TCTAGA T^CTAGA XmaCI 4136 CCCGGG C^CCGGG XmaI 4136 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccIII 51 TCCGGA T^CCGGA 1274 TCCGGA AcsI 4 GAATTC [GA]^AATT[CT] 4517 AAATTC AflII 3607 CTTAAG C^TTAAG 4678 CTTAAG ApoI 4 GAATTC [GA]^AATT[CT] 4517 AAATTC AvrII 2424 CCTAGG C^CTAGG 5433 CCTAGG BanI 3826 GGCGCC G^G[CT][GA]CC 5059 GGCACC BciVI 3384 GGATAC GTATCC(6/5) 2531 GTATCC BclI 353 TGATCA T^GATCA 4469 TGATCA BfrI 3607 CTTAAG C^TTAAG 4678 CTTAAG BlnI 2424 CCTAGG C^CTAGG 5433 CCTAGG BseAI 51 TCCGGA T^CCGGA 1274 TCCGGA Bsp1407I 4175 TGTACA T^GTACA 5147 TGTACA BspEI 51 TCCGGA T^CCGGA 1274 TCCGGA BsrGI 4175 TGTACA T^GTACA 5147 TGTACA BstBI 2077 TTCGAA TT^CGAA 3746 TTCGAA DraIII 721 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 2013 CACCTTGTG EcoRV 886 GATATC GAT^ATC 1537 GATATC FbaI 353 TGATCA T^GATCA 4469 TGATCA HindIII 3548 AAGCTT A^AGCTT 4427 AAGCTT MroI 51 TCCGGA T^CCGGA 1274 TCCGGA NdeI 599 CATATG CA^TATG 2592 CATATG NspV 2077 TTCGAA TT^CGAA 3746 TTCGAA PaeR7I 1264 CTCGAG C^TCGAG 1870 CTCGAG SexAI 1160 ACCTGGT A^CC[AT]GGT 2633 ACCTGGT SfuI 2077 TTCGAA TT^CGAA 3746 TTCGAA SphI 3797 GCATGC GCATG^C 4949 GCATGC SspBI 4175 TGTACA T^GTACA 5147 TGTACA XhoI 1264 CTCGAG C^TCGAG 1870 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AocI ApaI Asp700I AspHI AvaI AvaII BalI BamHI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI